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miRNA Expression in White Blood Cells

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The size, and in some cases the low expression levels, of small RNAs can make their analysis difficult. The small size of these molecules alone precludes the use of RT-PCR as a detection method, since they are too short to be specifically primed for PCR-mediated amplification. Furthermore, most RNA isolation procedures have been optimized to recover long (>500 nt) messenger RNAs, while ignoring smaller molecules. As a result, conventional RNA extraction methods can result in the loss of substantial amounts of small RNAs from samples. To address the issues of small RNA recovery and detection, Ambion scientists have developed the mirVana line of products, a group of kits designed to provide a complete solution for small RNA analysis.

The mirVana miRNA Isolation Kit (patent pending) was codeveloped with the mirVana miRNA Detection Kit to provide quantitative yields of small RNAs (including miRNA, siRNA, tRNA, and rRNAs) from virtually any biological sample. The patented mirVana miRNA Detection Kit is a set of reagents for small-volume solution hybridization assays that is far more efficient than membrane-based hybridization (e.g. Northern blots). The assay can detect relatively abundant miRNA species, such as miR-124 in brain, in as little as 10 ng
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TAG: miRNA Expression White Blood Cells

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