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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

9,262 2,884 31.1 Un08 195 5,198 1,873 36.0 Un09 98 3,856 714 18.5 Un10 49 2,690 814 30.3 Un11 24 2,208 179 8.1

* Mean concentration (cells/well) of cells two days after plating is calculated.


METHOD FOR PERFORMING THE CYQUANT ASSAY USING A DNA-BASED STANDARD CURVE AND RNASE-TREATED CELLS
Cell proliferation can also be assayed based on DNA content using a DNA standard curve. The standard curve can serve to quantitate cellular DNA, provided the cell lysates are pretreated with DNase-free RNase to prevent the RNA from contributing to the fluorescent signal. In this application note, instructions are provided for setting up the DNA standard curve and RNase-treated cell samples.


Prepare the assay reagents
Step 1. Dilute the concentrated cell-lysis buffer stock solution 1:20 with distilled water (prepare 200 L per well) to make 1X lysis buffer work solution. Prepare two types of 1X lysis buffer: one with a final concentration of 1mM EDTA and 180 mM NaCl and one without EDTA or NaCl.

Step 2. Prepare 1X and 2X working solutions (100 L per well) of CyQUANT GR dye/ cell-lysis buffer by diluting the concentrated CyQUANT GR stock solution 1:400 or 1:200, respectively, with working streng
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