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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

CELL-BASED STANDARD CURVE
A cell-based standard curve, set up as described above, is shown in Figure 3. A log-log curve fit was used when graphing the standard curve over the entire dynamic range of the assay. Results shown are from the SpectraMax M5 microplate reader, but similar results were obtained with SpectraMax M2 and Gemini EM or XPS.

Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Cells/well on day two were calculated with SoftMax Pro using the cell-based standard curve.

Table 3. Example Of Unknowns Group Data* Sample # Cells Plated
Day 0
Mean
Cell #
Day 2
Std.
Dev.
CV% Un01 50,000 58,861 6,255 10.6 Un02 25,000 56,822 8,055 14.2 Un03 12,500 54,860 5,203 9.5 Un04 6,250 47,854 7,548 15.8 Un05 3,125 32,746 6,257 19.1 Un06 1,562 23,036 4,533 19.7 Un07 391
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Mol wt: monoisotopic mol wt1,045.5423 Da by calculation...
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