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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

p-down menu to locate and open the CyQUANT Fluorescence protocol located in the Cytoproliferation folder.

Step 2. Click the Setup button in the Plate section to launch the Instrument Settings dialogue box as shown in Table 2. After setting all of the parameters, click OK to proceed.

Table 2. Instrument Settings Dialog
Options
Read Type Fluorescence
Top read Wavelengths Ex 485
Em 538
Cutoff 515 Sensitivity Readings: 30
PMT: Auto Automix Before: 5 secs AutoCalibrate On Assay Plate Type 96-well standard clear-bottom
(default)
96-well standard opaque Wells To Read [Determined by user] Settling Time Off AutoRead Off
Note: Instrument settings for SpectraMax M5. SpectraMax M2 and Gemini setup options in Fluorescence mode are identical, except for the absence of the Settling Time option.

Step 3. Create a template of the assay indicating the location of standards (cell numbers for each dilution), blanks (no cell lysate) and unknowns in the microplate. Click the Template button in the Plate section to set up the template. An examp
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