Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

te lysis of the cells.


Prepare the assay reagents
Step 1. Dilute the concentrated cell-lysis buffer stock solution 1:20 with distilled water (prepare 200 L per well) to make 1X celllysis buffer.

Step 2. Prepare 1X working solution of CyQUANT GR dye/cell-lysis buffer by diluting the concentrated CyQUANT GR stock solution 1:400 with 1X cell-lysis buffer. This solution should be prepared in a plastic container rather than glass (see CyQUANT product insert).


Prepare cell-based standard curve and unknowns
Step 1. Thaw the previously prepared frozen cell pellet at room temperature for a few minutes. Add 1.0 mL of CyQUANT GR dye/cell-lysis buffer and resuspend the cells by vortexing briefly. Assuming the pellet contained 106 cells, the resulting cell lysate contains the equivalent of 106 cells/mL.

Step 2. Generate a dilution series in the wells of a microplate, starting at 50,000 cells/well and making serial 1:2 dilutions down to 24 cells/well. se CyQUANT GR dye/cell-lysis buffer to make dilutions such that the desired cell number is in a volume of 200 l. Prepare four replicates of each standard, and include a set of replicates without cells as a control (you can omit one of the standards, e.g., 781 cells/well, to make room on the microplate).

Step 3. Incubate the microplate at room temperature for 2-5 minutes, protected from light.


Set up the instrument and software
Step 1. Turn on the SpectraMax M5, M2, or Gemini microplate reader. Launch the SoftMax Pro software and use the Assays dro
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:8/27/2014)... DUBLIN , Aug. 27, 2014 ... the "Global Enzyme for Pulp & Paper Industry ... The Global Enzyme for Pulp & Paper Industry ... the current state of the global enzyme for pulp ... basic overview of the industry including definitions, classifications, applications ...
(Date:8/27/2014)... 27, 2014  ARCH Venture Partners, one of ... the development of seed and early-stage advanced technology ... closed with more than $400 million in subscriptions. ... subscription target by more than $150 million. ... strength and potential of our approach to finding ...
(Date:8/27/2014)... 2014 Riviera Beach, Florida based ... creation of the Sancilio Scientific Advisory Board (SSAB). ... philosophy of looking to nature for pharmaceutical advancements, ... are based on sound scientific data, analyses, expertise ... provides a vehicle for SCI to receive peer ...
(Date:8/27/2014)... After the boom of the energy ... beverage craze is going to be the stress relief ... use Stress Relief energy pattern which can be applied ... a beverage, including minerals, herbs and liquids. Stress Relief ... power into rapid recovery and helping to adapt more ...
Breaking Biology Technology:Global Enzyme for Pulp & Paper Industry Report 2014 2Latest ARCH Venture Partners Fund Closes Above $400 Million 2Latest ARCH Venture Partners Fund Closes Above $400 Million 3New Sancilio & Company, Inc. Scientific Board 2New Sancilio & Company, Inc. Scientific Board 3Stress Relief: 1 Way to De-Stress Your Beverage Formula 2Stress Relief: 1 Way to De-Stress Your Beverage Formula 3Stress Relief: 1 Way to De-Stress Your Beverage Formula 4
... step closer to creating a micro-aircraft that flies with the ... aerodynamic secrets of insect flight. Dr John Young, from ... and a team of animal flight researchers from Oxford University,s ... locusts in action in a wind tunnel, capturing how the ...
... , , SAN ... ("STI"), a privately-held and development-stage biopharmaceutical company with a ... human therapeutic antibodies for the treatment of a variety ... of QuikByte Software, Inc. (OTC Bulletin Board: QBSW), a ...
... , , , , ... independent, nonprofit research and development institute, announced today that John J. ... September 1, 2009. Young will become the SRI Board,s tenth ... the business community. , , "Throughout his career, ...
Cached Biology Technology:Secrets of insect flight revealed 2Sorrento Therapeutics, Inc. and QuikByte Software, Inc. Complete Merger 2Sorrento Therapeutics, Inc. and QuikByte Software, Inc. Complete Merger 3Sorrento Therapeutics, Inc. and QuikByte Software, Inc. Complete Merger 4John J. Young, Jr. Joins SRI Board of Directors 2John J. Young, Jr. Joins SRI Board of Directors 3
(Date:8/27/2014)... the Volunteer Wildfire Services of South Africa as "Cape ... residents in certain parts of the province on Monday ... high veld fire danger rating is expected in the ... Kei and Mnquma area. Strong winds often occurred ... bring lightning strikes and subsequent fire and the wind ...
(Date:8/27/2014)... number of consumers are willing to pay a premium ... whether they,re getting what the label claims is another ... devising a new way to make sure farms are ... ACS, Journal of Agricultural and Food Chemistry , ... the Bavarian Health and Food Safety Authority and the ...
(Date:8/27/2014)... of studies have shown that excessive iron ... Parkinson,s disease. Previous studies from Chunyan Guo ... China have shown that baicalin prevented iron ... metal transporter 1 expression, and increased ferroportin ... rotenone-induced Parkinson,s disease rats. However, the relationship ...
Breaking Biology News(10 mins):
... night blindness, an inherited condition that affects one,s ability to ... mutation in a calcium channel protein that shuttles calcium into ... University School of Medicine have teased apart the molecular mechanism ... cells control calcium levels. The discovery, published in the Feb. ...
... a serious toll on plants and animals alike. When cells ... and, without water as a medium, chemicals and enzymes inside ... named "resurrection fern" ( Polypodium polypodioides ), can survive extreme ... their water content. How do the cells in these desiccation-tolerant ...
... March 18, 2010. How did China and Australia develop ... Europe? A panel of U.S. experts will try to ... a workshop at the National Science Foundation (NSF) in Arlington, ... the National Science Foundation and other Federal agencies, the World ...
Cached Biology News:Understanding night blindness and calcium 2Bringing dehydrated plants 'back to life' 2Bringing dehydrated plants 'back to life' 3Workshop on R&D for rapid vaccine development and production in Asia/Australia 2
Conjugation services for Antibodies, Proteins, Oligos, Peptides, Beads, bioconjugation, antibody-oligonucleotide, LNA peptide, LNA protein, LNA antibody, HRP, Alkaline phosphatase, phycoerythrin, FIT...
SCP-1 (P-17)...
Immunogen: Purified native p24 from strain IIIB. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biotin d anti-mouse Tim-1...
Biology Products: