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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

195 98 49 24 no cells D 50,000 25,000 12,500 6,250 3,125 1,562 391 195 98 49 24 no cells

Step 2. Incubate the microplate at 37C for the desired time after which proliferation will be assayed. The incubation time will depend on the type of proliferation assay being performed, the cell type being used and the users particular experimental design.

Step 3. Gently aspirate culture medium from wells. The wells may be washed gently with phosphate buffered saline (PBS), but this is not essential and is not recommended for very dense cultures where cells may dislodge.

Step 4. Freeze the cells in the microplate at -70C until ready to assay (at least one hour, up to four weeks). This freezing step ensures complete lysis of cell samples.


Prepare cells for the standard curve
Note: It is best to use the same type of cell for the standard curve as was used in the experiment.

Step 1. Detach adherent cells from the culture plate using trypsin or EDTA solution, and prepare a concentrated cell suspension in culture medium. Cells should be at a density of about 105 to 106 cells/mL.

Step 2. Centrifuge 1.0 mL of the cell suspension for 5 minutes at about 200 x g (approximately 1500 rpm in a microcentrifuge). Discard the supernatant and freeze the cell pellet at -70C until ready to assay. This freezing step ensures comple
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