Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

195 98 49 24 no cells D 50,000 25,000 12,500 6,250 3,125 1,562 391 195 98 49 24 no cells

Step 2. Incubate the microplate at 37C for the desired time after which proliferation will be assayed. The incubation time will depend on the type of proliferation assay being performed, the cell type being used and the users particular experimental design.

Step 3. Gently aspirate culture medium from wells. The wells may be washed gently with phosphate buffered saline (PBS), but this is not essential and is not recommended for very dense cultures where cells may dislodge.

Step 4. Freeze the cells in the microplate at -70C until ready to assay (at least one hour, up to four weeks). This freezing step ensures complete lysis of cell samples.


Prepare cells for the standard curve
Note: It is best to use the same type of cell for the standard curve as was used in the experiment.

Step 1. Detach adherent cells from the culture plate using trypsin or EDTA solution, and prepare a concentrated cell suspension in culture medium. Cells should be at a density of about 105 to 106 cells/mL.

Step 2. Centrifuge 1.0 mL of the cell suspension for 5 minutes at about 200 x g (approximately 1500 rpm in a microcentrifuge). Discard the supernatant and freeze the cell pellet at -70C until ready to assay. This freezing step ensures comple
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:1/23/2015)... 23, 2015 Biofertilizers stimulate microbial activities ... nitrogen and phosphorous through the activities in the soil ... increasing due to the stress on reducing environmental pollution ... The report defines and segments the bio fertilizers market ...
(Date:1/22/2015)... 22, 2015  Transwestern | RBJ today announces the firm brokered ... for Shire a leading biopharmaceutical company, at Two Ledgemont Center ... Robert Richards , president, and Brian Cohen , ... entire five-floor building at 95 Hayden Ave. Photo ...
(Date:1/22/2015)... Protocol Networks brings independent technology consulting to the ... consultants, Protocol Networks has recently announced its expansion into ... his company has attracted several clients in The Constitution ... as others. With the success of these engagements, Rogers ...
(Date:1/22/2015)... 22, 2015 Controlled Substance Compliance Services ... helps companies check the legal requirements around using controlled ... China. , As their international operations expand and ... software solutions built as a result of the first ...
Breaking Biology Technology:Biofertilizers Market Expected to Reach $1,649.7 Million by 2019 - New Report By MarketsandMarkets 2Biofertilizers Market Expected to Reach $1,649.7 Million by 2019 - New Report By MarketsandMarkets 3Biofertilizers Market Expected to Reach $1,649.7 Million by 2019 - New Report By MarketsandMarkets 4Transwestern | RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 2Global Compliance Service for Controlled Substances to Expand to China 2
... Biotechnology Company Adopts Pharsight,s Groundbreaking ... Automation Software, ... a leading provider of software and strategic,services for optimizing clinical drug ... WinNonlin AutoPilot.,This sale marks Pharsight,s third new customer win since the ...
... /PRNewswire-FirstCall/ - GeneNews Limited (TSX: GEN),a company ... for the early detection of diseases and ... started collecting patient samples,for clinical validation studies ... In June 2006, GeneNews presented training set ...
... 17 /PRNewswire-FirstCall/ - Helix BioPharma Corp.,(TSX, FSE: "HBP") ... purchase, by way of private placement, of a ... for gross proceeds totaling CDN$16,867,200.,Proceeds from the placement ... the Company,s research and development initiatives., The ...
Cached Biology Technology:Pharsight Signs Third WinNonlin(R) AutoPilot(TM) Customer 2Pharsight Signs Third WinNonlin(R) AutoPilot(TM) Customer 3GeneNews initiates patient sample accrual for validation studies of colon cancer blood test 2GeneNews initiates patient sample accrual for validation studies of colon cancer blood test 3Helix BioPharma announces $16.9 million private placement of common shares 2
(Date:1/22/2015)... Research and Markets ( http://www.researchandmarkets.com/research/wxdglm/global_wearable ) ... Wearable Technologies Market and Applications, Opportunities, Industry Analysis, ... 2014-2020" report to their offering. , ... can be worn on the user,s body and ...
(Date:12/22/2014)... SHELTON, Conn. , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: ... a biometric authentication company focused on the growing mobile commerce ... consultant, Apollo Robbins for the 2015 International CES ... Apollo Robbins will be at the NXT-ID booth ...
(Date:12/19/2014)... and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has announced the ... Security Systems Market" report to their offering. ... perimeter security market is estimated to grow at a CAGR ... holds a larger share in this market, Canada ...
Breaking Biology News(10 mins):Global Wearable Technologies Market and Applications, Opportunities, Industry Analysis, Segmentation, Size, Growth, Share, Overview, Trends and Forecast 2014-2020 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3
... And, at least for mice, it's one that is best made ... designed help scientists understand the brain chemicals that guide mate selection, ... a male mouse alone or a male mouse with a female. ... females. , "Our data suggest that female mice may use, or ...
... Infectious Diseases (NIAID), part of the National Institutes ... a large international HIV/AIDS trial comparing continuous antiretroviral ... of CD4+ cells has been stopped. Enrollment was ... twice the risk of disease progression (the development ...
... Texas M. D. Anderson Cancer Center have created a ... to find, highlight, and deliver genes to tumors in ... journal Cell, is potentially an important step in making ... it may also allow prediction and monitoring of how ...
Cached Biology News:When mice choose mates, experience counts 2International HIV/AIDS trial finds continuous antiretroviral therapy superior to episodic therapy 2International HIV/AIDS trial finds continuous antiretroviral therapy superior to episodic therapy 3International HIV/AIDS trial finds continuous antiretroviral therapy superior to episodic therapy 4New hybrid virus provides targeted molecular imaging of cancer 2
Agarose, pulse field, 100 g. Nuclease-free.Gel point (1.5%): 36 1.5 C, EEO (-mr): 1800 g/cm2. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
Molecular Biological Resources has extensive abilities cloning and expression of prokaryotic and eukaryotic proteins. Please contact lloconne@molbiores.com to discuss your project. Contact lloconne@...
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
quantitation of phosphatase activity, evaluation of hits and leads from HTS...
Biology Products: