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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

d to microplates for assay. Please refer to the CyQUANT Cell Proliferation Assay Product Information sheet MP-7026 from Molecular Probes for details.


METHOD FOR PERFORMING THE CYQUANT ASSAY USING A CELL-BASED STANDARD CURVE
Prepare cells to be assayed for proliferation
Step 1. Detach adherent cells from the culture plate using a trypsin or EDTA solution, and use culture medium to dilute the cell suspension into a 96-well microplate, making the cell concentrations (number of cells/well) shown in the template setup in Table 1. Include a set of control wells without cells.

Table 1. Cell Concentration (Number Of Cells) Prepared In A Microplate Well 1 2 3 4 5 6 7 8 9 10 11 12 A 50,000 25,000 12,500 6,250 3,125 1,562 391 195 98 49 24 no cells B 50,000 25,000 12,500 6,250 3,125 1,562 391 195 98 49 24 no cells C 50,000 25,000 12,500 6,250 3,125 1,562 391
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