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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

ese conditions is from 50 to 50,000 cells, as expected based on Molecular Probes Product Information sheet MP 7026. A comparison of RFUs in RNase-treated and untreated cell samples is shown in Figure 6 and demonstrates that RNase treatment reduces the amount of fluorescence in the cell lysates.


CONCLUSIONS
The CyQUANT Cell Proliferation Assay is a quick, sensitive fluorescence method for quantitating cell numbers or cellular DNA content. Assay results are similar on the SpectraMax M5 or M2, and Gemini XPS or EM microplate readers. Using a cell-based standard curve and a five-minute incubation, we observed a limit of detection and dynamic range similar to that described in Molecular Probes CyQUANT Product Information sheet (50-50,000 cells per sample). Using a DNA standard curve generated with the l DNA standard provided with the kit, we were able to calculate the amount of DNA in as few as 50 cells. The analysis capabilities of SoftMax Pro software provide a flexible, convenient method for calculating and reporting the data.


REFERENCES
1. Molecular Probes Product Information sheet MP 7026 1/12/01: CyQUANT Cell Proliferation Assay Kit (C-7026).
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