Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

ese conditions is from 50 to 50,000 cells, as expected based on Molecular Probes Product Information sheet MP 7026. A comparison of RFUs in RNase-treated and untreated cell samples is shown in Figure 6 and demonstrates that RNase treatment reduces the amount of fluorescence in the cell lysates.


CONCLUSIONS
The CyQUANT Cell Proliferation Assay is a quick, sensitive fluorescence method for quantitating cell numbers or cellular DNA content. Assay results are similar on the SpectraMax M5 or M2, and Gemini XPS or EM microplate readers. Using a cell-based standard curve and a five-minute incubation, we observed a limit of detection and dynamic range similar to that described in Molecular Probes CyQUANT Product Information sheet (50-50,000 cells per sample). Using a DNA standard curve generated with the l DNA standard provided with the kit, we were able to calculate the amount of DNA in as few as 50 cells. The analysis capabilities of SoftMax Pro software provide a flexible, convenient method for calculating and reporting the data.


REFERENCES
1. Molecular Probes Product Information sheet MP 7026 1/12/01: CyQUANT Cell Proliferation Assay Kit (C-7026).
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:7/10/2014)... 10, 2014  Franciscan St. Anthony Health-Crown ... for respiratory monitoring outside the operating room, ... in embracing state-of-the-art patient safety technology. ... breathing and can alert medical caregivers when ... amount of carbon dioxide the patient is ...
(Date:7/10/2014)... -- Research and Markets ( http://www.researchandmarkets.com/research/s3bqj2/photonic ) has ... Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... traces its roots in the latter half of ... went unexplored and unfulfilled for several decades owing ...
(Date:7/10/2014)... microscopic polymer light resonators that expand in the ... Photonics Laboratory have developed new optical sensors with ... sensors are ideal for detecting trace gas concentrations ... nature, and immunity to electromagnetic interference. , Although ... the MIT team conceived an extremely sensitive, compact ...
(Date:7/10/2014)... that power our laptops and electric vehicles could store ... with the help of a sponge-like silicon material. ... graphite traditionally used in one of the battery,s electrodes, ... storage capacity of graphite. A paper describing the material,s ... in Nature Communications . , "Silicon has long ...
Breaking Biology Technology:Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2Swell new sensors 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3
(Date:7/11/2014)... The Chinese University of Hong Kong, BGI and other ... to salt tolerance, with implication for improving this important ... online in Nature Communications provides an effective ... , Soybean is an important crop for the world. ... less genetic diversities than their wild counterparts. Among the ...
(Date:7/11/2014)... group members to reduce aggression and gain access to ... grooming activities shows a certain pattern across the day. ... Biology Letters . , Grooming between individuals in ... motives. To be groomed has hygienic benefits and is ... can provide access to infants, mating opportunities and high ...
(Date:7/11/2014)... Ume University, Sweden, have explored two different ways ... sequence leading to the formation of oxygen molecules ... in the scientific journal Nature Communications . ... day synthetic catalysts for water oxidation, which are ... the direct storage of solar energy in fuels ...
Breaking Biology News(10 mins):BGI reports a novel gene for salt tolerance found in wild soybean 2Baboons groom early in the day to get benefits later 2Molecular snapshots of oxygen formation in photosynthesis 2
... Lockheed Martin (NYSE: LMT ) has been ... of the Year" by Frost and Sullivan, recognizing the ... high levels of customer value. "Lockheed ... that play an important role our customer,s law enforcement ...
... Nitrogen is essential for plant growth. Despite the abundance ... must rely on a series of chemical reactions to ... forms. Plants have achieved this goal primarily by evolving ... nitrogen, plants, and the environment are the focus of ...
... Has a bone density scan placed you at risk ... bone-building medication? Not so fast! A University of Illinois study ... dietary calcium and vitamin D or taking calcium and vitamin ... be a last resort," said Karen Chapman-Novakofski, a U of ...
Cached Biology News:Lockheed Martin Named Biometrics "Company of the Year" by Frost and Sullivan 2Book looks at interrelationships among nitrogen, plants and the environment 2U of I study: Before you start bone-building meds, try dietary calcium and supplements 2U of I study: Before you start bone-building meds, try dietary calcium and supplements 3
Performance, mycoplasma, virus, and endotoxin tested...
... 10X BlueJuice Gel Loading ... easy loading and tracking ... agarose or native polyacrylamide ... features higher density sucrose ...
... is the most effective ... PCR products. TOPO TA ... TOPO vectors are supplied ... topoisomerase I. This enables ...
ready-to-use solution...
Biology Products: