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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

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Table 5. Sample Group Table: Cellular DNA Concentrations Calculated By SoftMax Pro Software Using DNA Standard Curve* Cells/ Well Mean RFU Mean Cell DNA (ng) Std. Dev. Cell DNA CV (%) 50,000 554.8 40.29 1.20 3 25,000 375.6 26.40 0.40 1.6 12,500 195.8 13.04 0.46 3.5 6,250 106.1 6.71 0.34 5 3,125 58.5 3.52 0.24 6.8 1,563 30.7 1.75 0.12 6.7 781 17.0 0.93 0.16 17.4 391 7.1 0.36 0.08 21.8 195 6.2 0.31 0.05 14.9 98 3.0 0.14 0.04 27.8 49 2.7 0.13 0.03 20.8

* Replicates are not presented in this view of the group table.

Figure 5 shows the average cellular DNA in each set of samples versus the number of cells/well. The linear range of the assay under th
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