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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

the tool bar of the Plate section.


Read the plate and analyze the data
Step 1. Place the microplate in the reader.

Step 2. Read the plate. RFUs will be displayed in the Plate section.

Step 3. After the microplate has been read, the RFUs will be displayed in the Plate section. The data will be analyzed in the Group tables that you created while setting up the template.

Step 4. Plot the mean RFU of the Standards group versus the concentration of the Standards group in the Graph section for the DNA standard curve.

Step 5. Choose the appropriate curve fit from the drop-down Curve Fit menu in the Graph sections tool bar. We used a log-log curve fit for this experiment.

Step 6. Calculate the cellular DNA values in RNase-treated cells from the DNA standard curve using the built-in formula in SoftMax Pro. An example of the Graph Options and Edit dialogue boxes are shown in Figure 2.


RESULTS FOR DNA STANDARD CURVE
A typical DNA-based standard curve is shown in Figure 4. The SpectraMax M5 microplate reader was used to generate these data, but similar results were obtained on the SpectraMax M2 and Gemini microplate readers (data not shown). An example of the calculation of cellular DNA concentrations in RNase-treated cells from a DNA standard curve is shown in Table 5. For this application note, a serial cell dilution was used as unknowns. In fact, any number of cells between 50 and 50,000 cells/well (in the linear range of the standard curve) can be detected and cellular DNA value can be obtained using this method. '"/>

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