Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

a microplate. Add 4 L of RNase (two units per well; see note below) into each well containing cells as well as the control wells without cells. Incubate at room temperature for one hour.

Note: DNase-free RNase should be used at a concentration such that no more than 10 L contains the full number of units needed to treat a sample. For example, the RNase Cocktail from Ambion has 500 U/ mL activity, so 4 L per well equals two units per well.

Step 3. Add 100 L of 2X CyQUANT GR dye/ cell-lysis buffer to each of the microplate wells, including the control wells without cells.

Step 4. Prepare the DNA standard curve by transferring the previously prepared DNA serial dilutions into the appropriate wells of the microplate, as defined in the template editor. The plate should contain two controls: a digest control prepared following the same steps as the cellular samples, but without cells (no DNA), and a standard curve control/plate blank containing only 200 L 1X CyQUANT GR dye/cell-lysis buffer (no DNA and no RNase).

Step 5. Incubate the samples for 2-5 minutes.


Set up the instrument and software
Step 1. Turn on the microplate reader. Launch the SoftMax Pro software and use the Assays drop-down menu to locate and open the Basic endpoint protocol in the Basic protocols folder.

Step 2. Set up the Instrument Settings dialog box as shown in Table 2.

Step 3. Create a template of the assay showing where standards, unknowns (cells treated with RNase), and plate blanks will be located on the microplate. To access the Template Editor, click the Template button in
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:7/24/2014)... 24, 2014 SRI International has been awarded ... Institute of Allergy and Infectious Diseases (NIAID), part of ... of potential therapies for HIV infection and AIDS. The ... and AIDS and the complications and opportunistic infections associated ... sexual transmission of HIV. According to ...
(Date:7/24/2014)... 2014 Deep Research Report on Global ... and in-depth research report on Potassium Sulphate ... information, including its definition, classification, application, and industry ... the international market analysis, including China’s domestic market ... environment & economic situation analysis, the influence of ...
(Date:7/24/2014)... July 24, 2014 1000 balances – ... input on customers’ weighing needs and expertly narrows down ... of balances, scales or even terminals. , First, ... smallest weight (or select readability instead) and define the ... additional selection criteria narrows down the list to models ...
(Date:7/24/2014)... July 24, 2014  Spherix Incorporated (SPEX) -- an ... monetization of intellectual property, today announced that the Company ... June 30, 2014 on Monday, August 18, 2014 for ... a.m. ET on Monday, August 18, 2014 to discuss ... the company,s CEO, Anthony Hayes . ...
Breaking Biology Technology:National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 4Spherix Schedules Shareholder Update Call 2
... agricultural equipment manufacturer Gehl Co. has developed a ... boom functions using a compact, wireless remote control pendant from ... The hand-held pendant can be used to raise, lower, extend ... , ,"Our goal was to add a new dimension ...
... Established and emerging biotech companies in Wisconsin's biotechnology sector ... upcoming Wisconsin Biotechnology and Medical Device Association Conference ... at the Olympia Resort in Oconomowoc. , ,"We're holding ... civic and business leaders of both cities to come," ...
... business. Many companies maintain battalions of HR professionals dedicated ... the right skills to the right job. , ... all manner of testing and diagnostic services designed to ... of potential hires. Countless hours are expended in endless ...
Cached Biology Technology:State of biotech, medical device sector is focus of upcoming conference 2Hiring in the name of talent 2Hiring in the name of talent 3Hiring in the name of talent 4
(Date:7/25/2014)... The routine use of a molecular testing panel ... performing the correct initial surgery for patients with ... University of Pittsburgh Cancer Institute (UPCI), partner with ... Multidisciplinary Thyroid Center and other diagnostic testing agencies, ... initial surgery by 30 percent, according to the ...
(Date:7/24/2014)... Scientists at The New York Stem Cell Foundation ... creating a viable cell replacement therapy for multiple ... For the first time, NYSCF scientists generated induced ... of patients with primary progressive multiple sclerosis and ... these stem cells into becoming oligodendrocytes, the myelin-forming ...
(Date:7/24/2014)... 24, 2014 /PRNewswire-iReach/ -- The Geneva Healthcare Suite, an ... has cut emergency room wait times by an average ... Medical Center, according to a recent study published in ... are using the suite to access data from incoming ... "Using Geneva,s technology platform we have been ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3NYSCF scientists one step closer to cell therapy for multiple sclerosis patients 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 3
... A novel study reports that white men and women of ... valgus) and lesser toe deformities, including hammer or claw toe. ... heritability of foot disorders in humansappear in Arthritis Care ... of the American College of Rheumatology (ACR). Previous ...
... California, Davis have engineered a strain of photosynthetic cyanobacteria ... report their findings today at the 113th General Meeting ... work, we used synthetic biology approaches to probe and ... energy for growth) cyanobacterial metabolism for the ability to ...
... 2013) Research presented today at Digestive Disease Week (DDW) ... the impact of coffee on autoimmune disease and palliative care ... been associated with reduced risk of fibrosis, a new study ... month also correlate with lower risk for a particular autoimmune ...
Cached Biology News:Blame your parents for bunion woes 2Coffee consumption associated with reduced risk of autoimmune liver disease 2
... GCpro Taq DNA Polymerase is a ... advantages for all PCR applications. GCpro Taq ... clone expressed in E. coli, containing the ... The enzyme includes a highly processive 5'-3' ...
Ps-Aeruginosa (IgG) -Ab EIA Sample Size: 100 l...
...
Automated -80° C sample library for microplates and cryotube racks in SBS format; for integration into automated processes....
Biology Products: