Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

a microplate. Add 4 L of RNase (two units per well; see note below) into each well containing cells as well as the control wells without cells. Incubate at room temperature for one hour.

Note: DNase-free RNase should be used at a concentration such that no more than 10 L contains the full number of units needed to treat a sample. For example, the RNase Cocktail from Ambion has 500 U/ mL activity, so 4 L per well equals two units per well.

Step 3. Add 100 L of 2X CyQUANT GR dye/ cell-lysis buffer to each of the microplate wells, including the control wells without cells.

Step 4. Prepare the DNA standard curve by transferring the previously prepared DNA serial dilutions into the appropriate wells of the microplate, as defined in the template editor. The plate should contain two controls: a digest control prepared following the same steps as the cellular samples, but without cells (no DNA), and a standard curve control/plate blank containing only 200 L 1X CyQUANT GR dye/cell-lysis buffer (no DNA and no RNase).

Step 5. Incubate the samples for 2-5 minutes.


Set up the instrument and software
Step 1. Turn on the microplate reader. Launch the SoftMax Pro software and use the Assays drop-down menu to locate and open the Basic endpoint protocol in the Basic protocols folder.

Step 2. Set up the Instrument Settings dialog box as shown in Table 2.

Step 3. Create a template of the assay showing where standards, unknowns (cells treated with RNase), and plate blanks will be located on the microplate. To access the Template Editor, click the Template button in
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:5/17/2013)... Dr. Sparano is Professor of Medicine ... the Albert Einstein College of Medicine and Associate Chairman ... Center. He is also Associate Director for Clinical ... Einstein Breast Cancer Working Group, a multidisciplinary group of ... He also serves as Vice Chair of the National ...
(Date:5/17/2013)... The new Public-Private Partnership (PPP) in Photonics established ... annual meeting drew praise from community leaders from SPIE ... the action “a huge step forward … a lasting ... , The Board of Stakeholders voted to approve in ... its 29 April meeting in Brussels, which was sponsored ...
(Date:5/17/2013)... , May 17, 2013 /PRNewswire-iReach/ -- Aridis ... been reached with Switzerland -based ... anti-infective human monoclonal antibody (mAb) products, and technologies. ... mAb products for treatment of infections by common ... Staphylococcus aureus , Pseudomonas aeruginosa , ...
(Date:5/17/2013)... 17, 2013 Rust removal is one of ... home items could be damaged. To help rust contractors make ... its no-scrubbing rust remover it called Rusterizer. It ... with 10% discount. , My Cleaning Products explained that rust ... or by staining them. The first one, it said, ...
Breaking Biology Technology:Joseph A. Sparano, MD, Named Vice Chair of ECOG-ACRIN Cancer Research Group 2Joseph A. Sparano, MD, Named Vice Chair of ECOG-ACRIN Cancer Research Group 3SPIE Joins in Praise for ‘Huge Step Forward’ for Innovation R&D Enabled by Photonics21 Actions 2SPIE Joins in Praise for ‘Huge Step Forward’ for Innovation R&D Enabled by Photonics21 Actions 3Aridis Pharmaceuticals Announces Acquisition of Monoclonal Antibody Products and Technologies From Kenta Biotech 2Aridis Pharmaceuticals Announces Acquisition of Monoclonal Antibody Products and Technologies From Kenta Biotech 3Organic-Based Rust Remover Cuts Down Price by 10%, My Cleaning Products Details Mechanics How to Get the Discount 2
... May 23, 2012 Reportlinker.com announces ... is available in its catalogue: ... to 2018 - Novel Oral JAK ... Drugs (DMARD) Will Drive Continued Growth ...
... Oxford, May 22, 2012 - Researchers in Ireland have ... glasses to produce high-precision molds for making tiny plastic ... be used in the next generation of computer memory ... In their article published in the latest edition of ...
... 2012   GeneLink, Inc. (OTCBB: GNLK.OB, "the ... financial results for the three months ended March 31, 2012. ... February 10, 2012 the Company closed on the sale of ... transaction entered into in October 2011. The sale price was ...
Cached Biology Technology:Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 2Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 3Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 4Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 5Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 6Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 7Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 8Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 9Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 10Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 11Arthritis Therapeutics Market to 2018 - Novel Oral JAK Inhibitors and Biologic Disease Modifying Anti-Rheumatic Drugs (DMARD) Will Drive Continued Growth Across Key Indications 12Making microscopic machines using metallic glass 2GeneLink Reports First Quarter 2012 Financial Results 2
(Date:5/17/2013)... An international team of scientists using a new ... inside a living frog embryo in greater detail than ... advance biological research and the search for new treatments ... Karlsruher Institut fr Technologie in Germany, in collaboration with ... Energy,s Argonne National Laboratory, released the most precise depiction ...
(Date:5/16/2013)... FASEB MARC (Maximizing Access to Research Careers) Program ... Endocrine Society (ENDO) 95th Annual Meeting in San ... are meant to promote the entry of students, ... mainstream of the basic science community and to ... ENDO 2013 Annual Meeting. , Awards are given ...
(Date:5/16/2013)... living closer to a fast food restaurant had a ... further away from fast food, according to researchers at ... this association was particularly strong among those with a ... in the American Journal of Public Health ... fast food restaurant, and among lower-income African-Americans, the density, ...
Breaking Biology News(10 mins):New X-ray method shows how frog embryos could help thwart disease 2Body mass index of low income African-Americans linked to proximity of fast food restaurants 2Body mass index of low income African-Americans linked to proximity of fast food restaurants 3
... 2011) Researchers looking to find a root cause for ... a novel investigative approach that borders on science fiction as ... the nation,s No. 1 killer. The National Institutes of ... Scripps Translational Science Institute (STSI) of San Diego and Sangamo ...
... wide variety of new cut flower cultivars and species ... University and the Association of Specialty Cut Flower Growers. ... United States and Canada, provides valuable production and marketing ... team from North Carolina State University undertook a multiyear ...
... Stephane Len, both researchers at the IRMES (Institut de ... France), have published their findings in Age , ... the evolution of performances in elite athletes and chess ... developed by the laboratory, and suggests that changes in ...
Cached Biology News:Scripps awarded $7.9 million NIH grant for research to find root cause of heart attack 2Scripps awarded $7.9 million NIH grant for research to find root cause of heart attack 3Scripps awarded $7.9 million NIH grant for research to find root cause of heart attack 4Extending the vase life of cut flowers: Pre-treatments and preservatives studied 2Evolution of sport performances follows a physiological law 2
Alexa Fluor 647 anti-mouse Qa-2...
Mouse monoclonal [0.T.81] to Lac1 ( Abpromise for all tested applications). Antigen: Recombinant TrpE fusion protein containing full length E. coli Lac1....
Peptide-affinity Purified Polyclonal Antibody to ICEBERG Peptide with sequence EEDPQLASKMGLH, from C Terminus of the protein sequence according to NP_067546...
3T3 cells (RSV-transformed) Source: Cell Lysate Form: Ready to Use Application: Western Blotting Control...
Biology Products: