Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

a microplate. Add 4 L of RNase (two units per well; see note below) into each well containing cells as well as the control wells without cells. Incubate at room temperature for one hour.

Note: DNase-free RNase should be used at a concentration such that no more than 10 L contains the full number of units needed to treat a sample. For example, the RNase Cocktail from Ambion has 500 U/ mL activity, so 4 L per well equals two units per well.

Step 3. Add 100 L of 2X CyQUANT GR dye/ cell-lysis buffer to each of the microplate wells, including the control wells without cells.

Step 4. Prepare the DNA standard curve by transferring the previously prepared DNA serial dilutions into the appropriate wells of the microplate, as defined in the template editor. The plate should contain two controls: a digest control prepared following the same steps as the cellular samples, but without cells (no DNA), and a standard curve control/plate blank containing only 200 L 1X CyQUANT GR dye/cell-lysis buffer (no DNA and no RNase).

Step 5. Incubate the samples for 2-5 minutes.


Set up the instrument and software
Step 1. Turn on the microplate reader. Launch the SoftMax Pro software and use the Assays drop-down menu to locate and open the Basic endpoint protocol in the Basic protocols folder.

Step 2. Set up the Instrument Settings dialog box as shown in Table 2.

Step 3. Create a template of the assay showing where standards, unknowns (cells treated with RNase), and plate blanks will be located on the microplate. To access the Template Editor, click the Template button in
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:9/30/2014)... Report Details Biosimilar drugs – ... Do you want to find sales potentials of ... for those biological drugs from 2014, helping you ... data, R&D trends, opportunities and selling prospects. In ... 2024 at overall world market, therapeutic class, molecule ...
(Date:9/30/2014)... September 30, 2014 Proove Biosciences ... is excited to announce a new genetic test built ... Proove Pain Perception Test will provide physicians with ... why pain tolerance levels are stratified between individuals. With ... whether a person has a genetic predisposition that will ...
(Date:9/30/2014)... BUDAPEST, Hungary , September 30, 2014 /PRNewswire/ ... libraries and intuitive browsing of chemistry in documents ... providing chemistry software solutions and consulting services for ... extract chemical information and generate Markush structures from ... securing an organizations intellectual property, however generating and ...
(Date:9/30/2014)... and ROCKVILLE, Md., Sept. 30, 2014 ... focusing on commercializing market-relevant biohealth innovations and increasing access ... , announced today that venture capitalist, Tania Fernandez ... strategic advisor. Dr. Fernandez will be a member of ... which will provide up to $50 million in seed ...
Breaking Biology Technology:Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2Seasoned Life Sciences Venture Capitalist Tania Fernandez Joins BHI to Help Manage New BioHealth Gap Fund 2
... 11, 2011 Novavax, Inc. (Nasdaq: NVAX ... by the Tech Council of Maryland (TCM), the largest ... communities of Maryland.  Novavax was recognized as the firm ... community and for its presence and leadership in Maryland. ...
... May 11, 2011 Verenium Corporation (Nasdaq: ... the development and commercialization of high-performance enzymes, ... will present at two upcoming conferences. Mr. ... Solutions Conference as part of the "Biofuels" panel discussion ...
... Ill., May 11, 2011 Naurex Inc., a clinical-stage ... psychiatry and neurology, today announced the completion of an ... Partners and Latterell Venture Partners.  Druid BioVentures, Genesys Capital, ... in the round.  Naurex will use the proceeds from ...
Cached Biology Technology:Novavax Named Biotechnology Firm of the Year by Tech Council of Maryland 2Verenium to Present at Upcoming Conferences 2Verenium to Present at Upcoming Conferences 3Naurex Completes $18 Million Series A Financing 2Naurex Completes $18 Million Series A Financing 3Naurex Completes $18 Million Series A Financing 4Naurex Completes $18 Million Series A Financing 5Naurex Completes $18 Million Series A Financing 6Naurex Completes $18 Million Series A Financing 7
(Date:9/30/2014)... your doctor to know what goes wrong with your muscles ... to know what "normal" actually is. That,s where a new ... FASEB Journal comes in. In the ... set of molecules that can help scientists "see" which genes ... more, they found never-before-detected gene activity and that men have ...
(Date:9/30/2014)... ARLINGTON HEIGHTS, Ill. (September 30, 2014) People who ... they can do to control their asthma besides properly ... to a new article in the Annals of ... the American College of Allergy, Asthma and Immunology (ACAAI), ... by examining their lifestyle. The woman described in the ...
(Date:9/29/2014)... DC Monday, September 29: Between 1970 and ... fish around the globe dropped 52 percent, says ... World Wildlife Fund (WWF). This biodiversity loss occurs ... resource use of high-income countries. , In addition ... report,s data point to other warning signs about ...
Breaking Biology News(10 mins):Scientists identify which genes are active in muscles of men and women 2Asthma symptoms kicking up? Check your exposure to air pollution 2Half of global wildlife lost, says new WWF report 2
... part of the Genome Canada Large-Scale Applied Research Project ... to 16 projects across the country, the researchers in ... with the aim of identifying genes or proteins for ... target cancers and other diseases. The first project, ...
... - New research from the University of Calgary,s Faculty ... diagnosis using an iPhone application with the same accuracy ... technology can be particularly useful in rural medical settings. ... neurologists, regardless of where the physicians and patients are ...
... has revealed how infection by a parasitic fungus dramatically changes ... leonardi ), causing them to become zombie-like and to die ... fungus. The multinational research team studied ants living high up ... research will be published in the BioMed Central open-access journal ...
Cached Biology News:OGI genomics researchers awarded $23 million 2OGI genomics researchers awarded $23 million 3iPhone can diagnose stroke: study 2Zombie ants have fungus on the brain, new research reveals 2
... electrophoresis into an automated, high-throughput operation with ... system is ideal for analyzing multiple PCR ... Fully automated, robot-compatible, and ready to put ... your high-throughput screening assignments as easy as ...
...
Fluorescence Preserving Media, 100 mL...
Size & Applications: 20 ml Storage Temp.: -20C Shelf Life: 12 months ...
Biology Products: