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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

th cell-lysis buffer. This solution should be prepared in a plastic container rather than glass, as recommended by Molecular Probes.


Prepare a DNA standard in plastic tubes:
Step 1. Dilute the 100 g/mL DNA standard from the kit in 1X CyQUANT GR dye/celllysis buffer to make a 1 g/mL stock solution.

Step 2. Dilute the 1 g/mL stock solution further to make a dilution series as shown in Table 4.

Table 4: Preparation Of Dilutions For DNA Standard Curve For The CyQUANT Cell Proliferation Assay Tube
#v
1x CQ GR/
Lysis
Buffer (L)
1 g/mL DNA in
1x CQ GR/Lysis
Buffer (L)
Final DNA
Concentration
(ng/mL)
1 1000 0 0 2 990 10 10 3 950 50 50 4 900 100 100 5 800 200 200 6 600 400 400 7 400 600 600 8 200 800 800 9 0 1000 1000
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