Navigation Links
measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

th cell-lysis buffer. This solution should be prepared in a plastic container rather than glass, as recommended by Molecular Probes.


Prepare a DNA standard in plastic tubes:
Step 1. Dilute the 100 g/mL DNA standard from the kit in 1X CyQUANT GR dye/celllysis buffer to make a 1 g/mL stock solution.

Step 2. Dilute the 1 g/mL stock solution further to make a dilution series as shown in Table 4.

Table 4: Preparation Of Dilutions For DNA Standard Curve For The CyQUANT Cell Proliferation Assay Tube
#v
1x CQ GR/
Lysis
Buffer (L)
1 g/mL DNA in
1x CQ GR/Lysis
Buffer (L)
Final DNA
Concentration
(ng/mL)
1 1000 0 0 2 990 10 10 3 950 50 50 4 900 100 100 5 800 200 200 6 600 400 400 7 400 600 600 8 200 800 800 9 0 1000 1000
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
2. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
3. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
4. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
5. Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)
6. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
7. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
8. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
9. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
10. Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)
11. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
Post Your Comments:
(Date:7/31/2014)... Alan Turing, the British mathematician (1912-1954), is famous for ... the 20th century. In 1936 he published a paper, ... first formal concept of a computer algorithm. He next ... designing the machines which cracked the German military codes, ... crucial battles. And in the late 1940,s he turned ...
(Date:7/31/2014)... 2014 The SNIS Foundation recognized its ... fund a translational research project.  The first award ... was presented at the Society of NeuroInterventional Surgery ... Colorado Springs, CO. ... neurovascular conditions, SNIS formed the SNIS Foundation, in ...
(Date:7/31/2014)... July 31, 2014 North Shore Towers, a ... partnered with GI Energy, one of the nation’s leading combined ... million upgrade of its 43-year-old CHP system. The original CHP ... an effort to extend the life of the plant, GI ... associated electrical and heat recovery equipment, while maintaining continuous operation ...
(Date:7/31/2014)... Senomyx , Inc. (NASDAQ: ... taste science technologies to discover, develop, and commercialize ... ingredient supply industries, today reported financial results for ... second quarter with $33.6 million in cash and ... past quarter Senomyx achieved another important milestone with ...
Breaking Biology Technology:A mathematical theory proposed by Alan Turing in 1952 can explain the formation of fingers 2A mathematical theory proposed by Alan Turing in 1952 can explain the formation of fingers 3SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 2SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 3North Shore Towers Partners with GI Energy to Install Turnkey Natural Gas-Fired Combined Heat and Power Plant 2SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 2SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 3SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 4SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 5SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 6SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 7SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 8SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 9SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 10SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 11SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 12SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 13SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 14SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 15SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 16
... 9, 2011 Anticipating a catastrophic increase in Alzheimer,s ... much-anticipated California State Plan for Alzheimer,s disease, a disease ...  The plan is a 10-year course of action with ... this growing health crisis.   Alzheimer,s will ...
... CHICAGO, March 9, 2011 GlobalCare Clinical Trials, ... America. GlobalCare provides selected clinical study visits at ... or alternate settings – for study patients to ... therapeutic areas, genomics and personalized medicine in all ...
... Palatin Technologies, Inc. (NYSE Amex: PTN ) announced ... OC Growth Stock Conference on Tuesday, March 15, 2011, at ... the Ritz Carlton Laguna Niguel in Dana Point, CA. ... of Palatin Technologies, will provide an update on Palatin,s corporate ...
Cached Biology Technology:California Leaders Confront Alarming Rise in Alzheimer's Cases 2California Leaders Confront Alarming Rise in Alzheimer's Cases 3GlobalCare Clinical Trials, Ltd Announces Company Expansion Into Latin America 2GlobalCare Clinical Trials, Ltd Announces Company Expansion Into Latin America 3GlobalCare Clinical Trials, Ltd Announces Company Expansion Into Latin America 4
(Date:7/31/2014)... beneath many of the roughly 156,000 gas stations ... corrosion in parts of underground gas storage tankscorrosion ... of groundwater, a source of drinking water. In ... reported many rapidly corroding gas storage tank components ... associated with use of gasoline-ethanol blends and the ...
(Date:7/31/2014)... models against the highly infectious and virulent bacterium, ... kills approximately 30,000 Americans annually. The research is published ... , In the study, the vaccine protected the ... by C. difficile , as well as from ... the human disease, after only two immunizations. , ...
(Date:7/31/2014)... identify and treat those with fibromuscular dysplasia (FMD). FMD ... arteries in the body, which can restrict blood flow to ... report appearing in August 2014 issue of The ... may not be limited to the arteries as currently believed. ... and joints, as well as evidence that inflammation may be ...
Breaking Biology News(10 mins):NIST corrosion lab tests suggest need for underground gas tank retrofits 2C. difficile vaccine proves safe, 100 percent effective in animal models 2Scientists discover biochemical mechanisms contributing to fibromuscular dysplasia 2
... Bill Middleton peer into the ancient Mexican past. In ... help build the most accurate and most detailed landscape ... where the Zapotec people formed the first state-level and ... off the street about Mexican archeology, theyll say Aztec, ...
... off of the University of Granada Lorgen, in collaboration with ... de las Nieves of Granada, has managed to develop a ... the eighth week of pregnancy separating the DNA found in ... been tested through a clinical trial in 120 pregnant women, ...
... ORCH ) today announced that Thomas A. Bologna, ... present at the 2008 Bank of,America Health Care Conference ... p.m. PDT., Mr. Bologna will provide a brief ... A link to the live webcast of Mr.,Bologna,s presentation ...
Cached Biology News:Archaeologist uses satellite imagery to explore ancient Mexico 2Archaeologist uses satellite imagery to explore ancient Mexico 3A new analysis method allows to find out the sex of the baby from the second month of pregnancy 2