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measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

SPECTRAMAX APPLICATION NOTE

Cathy Olsen, Ph.D., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089.


INTRODUCTION
The Molecular Devices SpectraMax M5 and M2, and Gemini XPS and EM are versatile microplate readers whose detection modes include fluorescence intensity. These instruments have dual monochromators, so the optimization of excitation and emission wavelengths can easily be performed via spectral scanning without the purchase of additional individual filters. Superior sensitivity in fluorescence mode allows the user to perform a wide variety of applications with a full spectrum of fluorophores.

Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth. The CyQUANT Cell Proliferation Assay Kit from Molecular Probes is a sensitive, rapid and convenient way to quantitate cell growth using a fluorescence microplate reader. CyQUANT GR dye binds to cellular nucleic acids, allowing cell numbers to be calculated from a standard curve. Because DNA-to-RNA ratios can vary over the course of the cell cycle, the CyQUANT kit allows users to determine cell numbers using RNase-digested cell lysates and a nucleic acid standard curve.

This application note describes how to use the CyQUANT kit with the SpectraMax M5, M2 and Gemini microplate readers and SoftMax Pro software from Molecular Devices. Two methods are detailed.
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