Navigation Links
measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

Evelyn McGown, Ph.D., Jinfang Liao, M.D., Ph.D., Mary Kassinos, Irina Osetinsky and Jayne Hesley, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089.

Introduction
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. They can be cloned in a diverse range of cells and organisms, from bacteria and yeast, to plants and mammals. The fluorescent proteins are stable, have minimal toxicity and have the ability to generate visible fluorescence in vivo without the need for external cofactors. Thus they can be used as molecular tags or as independent reporters to visualize, track and quantify many different cellular processes, including protein synthesis and turnover, protein translocation, gene induction and cell lineage.

The various proteins have different colors, so they can be used in multiplexed assays. They can be monitored by fluorescent microscopy and flow cytometry. If there is no need to physically sort the cells or to monitor intracellular migration, microplate fluorometry offers a preferable, more convenient and higher-throughput detection system. Below, we show that the Analyst GT multimode reader from Molecular Devices can easily and noninvasively measure fluorescent proteins in living cells.

We obtained three HEK-293 cell lines from BD Biosciences Clontech, each stably transfected with a different fluorescent protein. The purposes of the study were: 1) to determine the optimal instrument settings for three cell lines, and 2) to prepare dilution series of each cell line to estimate lower limits of detection (LLD).


MATERIALS
HEK-293 Cell Lines stably expressing fluorescent proteins were obtained from BD Biosciences Clontech:
AcGFPa variant of GFP cloned from Aequorea coerelescens (a jellyfish distinct from victoria)
ZsGreensimilar to GFP, but b
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
2. The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
3. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
4. Singlet Oxygen measurement
5. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
6. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
7. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
8. Performance of fluorescent dyes with LEADseeker Multimodality Imaging System
9. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
10. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
11. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
Post Your Comments:
(Date:2/27/2015)... February 27, 2015 FamilyFarms Group is ... Manager of State Line Farms, received the 2015 Tomorrow’s ... producer, under the age of 35, who has demonstrated ... an honor to win the Tomorrow's Top Producer Horizon ... won the Top Producer Award and learning from their ...
(Date:2/26/2015)... -- S&P Capital IQ (MHFI) announced today that it ... Therapeutics Holdings Inc . MabVax Therapeutics ... focused on the development of vaccine and antibody-based therapies ... cancer. MabVax has discovered a pipeline of human monoclonal ... by patients who have been immunized against targeted cancers. ...
(Date:2/26/2015)...   Synageva BioPharma Corp. (NASDAQ: ... products for rare disorders, today reported 2014 full ... key objectives, and financial guidance.  Synageva,s management team ... p.m. EST to review the financial results and ... today,s call by telephone, please dial (877) 445-4603 ...
(Date:2/26/2015)... Angeles, CA (PRWEB) February 26, 2015 ... an online demonstration to support life extension technologies and ... 8pm UTC, Universal Coordinated Time, on March 21st 2015, ... the world. , Eric Schulke, Founder of MILE, ... must act with urgency. The Movement for Indefinite Life ...
Breaking Biology Technology:Matt Sims Receives the 2015 Tomorrow’s Top Producer Horizon Award 2S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 5Synageva BioPharma Reports 2014 Full Year Financial Results 2Synageva BioPharma Reports 2014 Full Year Financial Results 3Synageva BioPharma Reports 2014 Full Year Financial Results 4Synageva BioPharma Reports 2014 Full Year Financial Results 5Synageva BioPharma Reports 2014 Full Year Financial Results 6Synageva BioPharma Reports 2014 Full Year Financial Results 7Synageva BioPharma Reports 2014 Full Year Financial Results 8Synageva BioPharma Reports 2014 Full Year Financial Results 9Indefinite Life Extension Activists Organize Online Demonstration 2Indefinite Life Extension Activists Organize Online Demonstration 3
... MADISON, WI - U.S. Representative Tammy Baldwin and Wisconsin ... will each receive federal grants of $2 million to ... ,Both based in Madison, Stratatech Corporation is ... skin, and Virent Technologies develops technologies that ...
... recently expressed concern to Department of Health and Human ... clinical research trials. , ,Institutional review boards are backlogged ... authorizations for use and disclosure of information, according to ... Indianapolis-based Eli Lilly. , ,In a response letter, ...
... NY - One certain consequence of the nearly $60 ... first bigwig out the door is Dr. Gran Ando, ... operations. Today, Celltech, one of Europe's biggest biotechnology companies, ... will see that the entire senior management of Pharmacia ...
Cached Biology Technology:Stratatech and Virent Win $4M in U.S. Grants 2Stratatech and Virent Win $4M in U.S. Grants 3Stratatech and Virent Win $4M in U.S. Grants 4Stratatech and Virent Win $4M in U.S. Grants 5Pfizer-Pharmacia Fallout Gives Celltech A CEO 2
(Date:2/10/2015)... 10, 2015  Alere Inc. (NYSE: ALR), a ... results for the quarter ended December 31, 2014. ... President of Alere said, "We made substantial progress ... the global leader in rapid diagnostics and delivering ... Health divestiture in early January enabled us to ...
(Date:2/5/2015)... 2015   Epic Sciences , a precision diagnostics company ... Murali Prahalad , Ph.D., president and CEO, is scheduled ... (PMWC) 2015: Silicon Valley, which is taking place at the ... on January 26-28, 2015. Dr. Prahalad ... Biomarkers." Last year, Epic Sciences was a finalist in the ...
(Date:2/5/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD and ... focused on the growing mobile commerce market, announces it ... Conference on January 29 th at 10:30 a.m. ... of NXT-ID will present and host a live Q&A ... and its full suite of biometric technologies. Investors can ...
Breaking Biology News(10 mins):Alere Inc. Announces Fourth Quarter 2014 Results 2Alere Inc. Announces Fourth Quarter 2014 Results 3Alere Inc. Announces Fourth Quarter 2014 Results 4Alere Inc. Announces Fourth Quarter 2014 Results 5Alere Inc. Announces Fourth Quarter 2014 Results 6Alere Inc. Announces Fourth Quarter 2014 Results 7Alere Inc. Announces Fourth Quarter 2014 Results 8Alere Inc. Announces Fourth Quarter 2014 Results 9Alere Inc. Announces Fourth Quarter 2014 Results 10Alere Inc. Announces Fourth Quarter 2014 Results 11Alere Inc. Announces Fourth Quarter 2014 Results 12Alere Inc. Announces Fourth Quarter 2014 Results 13Alere Inc. Announces Fourth Quarter 2014 Results 14Alere Inc. Announces Fourth Quarter 2014 Results 15Alere Inc. Announces Fourth Quarter 2014 Results 16Alere Inc. Announces Fourth Quarter 2014 Results 17Alere Inc. Announces Fourth Quarter 2014 Results 18Alere Inc. Announces Fourth Quarter 2014 Results 19Alere Inc. Announces Fourth Quarter 2014 Results 20Alere Inc. Announces Fourth Quarter 2014 Results 21Alere Inc. Announces Fourth Quarter 2014 Results 22Alere Inc. Announces Fourth Quarter 2014 Results 23Alere Inc. Announces Fourth Quarter 2014 Results 24Alere Inc. Announces Fourth Quarter 2014 Results 25Alere Inc. Announces Fourth Quarter 2014 Results 26Alere Inc. Announces Fourth Quarter 2014 Results 27Alere Inc. Announces Fourth Quarter 2014 Results 28Alere Inc. Announces Fourth Quarter 2014 Results 29Alere Inc. Announces Fourth Quarter 2014 Results 30Alere Inc. Announces Fourth Quarter 2014 Results 31Alere Inc. Announces Fourth Quarter 2014 Results 32Alere Inc. Announces Fourth Quarter 2014 Results 33Alere Inc. Announces Fourth Quarter 2014 Results 34Alere Inc. Announces Fourth Quarter 2014 Results 35Alere Inc. Announces Fourth Quarter 2014 Results 36
... R.I. [Brown University] When it comes to understanding ... flatworms have been a real thorn in scientists, sides. ... a group of worms called Acoelomorpha . This ... part of a much larger group called bilateral animals, ...
... A recent United States Army study found that excessive ... up to 70 percent of soldiers who are severely ... civilian population also suffers from HO following trauma or ... other tissues causing severe pain, reduced mobility and even ...
... Ninety per cent of older persons in rural Bangladesh ... collaborative study presented in a dissertation by Tamanna Ferdous ... are part of a larger international study, challenge the ... by United Nations in the Millenium Development Goals, e.g. ...
Cached Biology News:Research team finds first evolutionary branching for bilateral animals 2New study finds way to stop excessive bone growth following trauma or surgery 2Malnutrition among older adults in rural Bangladesh challenges the Millennium Development Goals 2
... Adaptor encodes a T7 promoter, lac ... translation initation codon, and an N-terminal Trx•Tag ... aa sequence that encodes thioredoxin. Fusion to ... solubility of many proteins that are typically ...
...
The LIC Duet™ Mini Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is designed for expression of a target protei...
Mouse monoclonal antibody to QARS - glutaminyl-tRNA synthetase...
Biology Products: