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hNT BlueNeurons Express b-Galactosidase at High ,Levels

darker blue color than the staining of the accessory cells. Furthermore, the staining pattern of neurons is uniform and homogeneous.

Figure 6A &6B

Figure 6A shows transfected clone B6 replate 3 neurons after 6 weeks of RA induction, treatment with mitotic inhibitors and purification (figure 1). This photomicrograph shows two clusters of neurons that are stained intensely blue. Again, the blue staining appears uniform and homogeneous. This is also apparent in Figure 6B, a larger magnification of the cluster on the upper left of Figure 6A. These results indicate that the beta-galactosidase gene is expressed at a sufficient level to yield intense blue staining of the transfected neurons.

Conclusions

hNT BlueNeurons have high levels of beta-galactosidase activity and stain dark blue in standard assays. This feature is especially useful for transplantation studies of CNS tissues, where the ability to stain for beta-galactosidase activity allows hNT BlueNeurons to be easily distinguished from surrounding cells or tissues.

REFERENCES

  1. Trojanowski, J.Q., et al. (1993) Exp. Neurol. 122: 283294.

  2. Mantione, J.R., et al. (1995) Brain Res. 671: 333337.

  3. Kleppner, S.R., et al. (1995) J. Comp. Neurol. 357: 618632.

  4. Miyazano, M., Lee, V.M.Y., and Trojanowski, J.Q. (1995) Lab. Investigat. 73: 111.

  5. Santoshi, K., et al. (1995) Lab. Investigat. 73: 636648.

  6. Lee, V.M., McGrogan, M.P., Lernhardt, W., and Huvar, A. (1994) Strategies 7: 2831.

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