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Generate High-Quality, Directional Plasmid cDNA Libraries

Produce larger primary libraries with new plasmid library construction kits

Quinn Lu Marie Callahan Tanya Hosfield Barry Neiditch Alan Greener John C. Bauer Mike Kobrin
Stratagene

Stratagenes pBluescript II, pcmv-script and pAD-GAL4-2.1 XR library construction kits offer directional cloning of cDNAs and high-efficiency transformation into Epicurian Coli XL10-Gold ultracompetent cells.* Plasmid cDNA libraries constructed with XL10-Gold cells yield larger primary libraries than libraries made with other E. coli transformation hosts, thereby increasing the probability of recovering full-length clones. Libraries constructed in these plasmid systems can be screened directly for gene homology or gene expression. Each of the three library construction kits is unique, designed specifically for prokaryotic expression, eukaryotic expression or two-hybrid screening in yeast.

pBluescript II SK(+) Vector

pCMV-ScriptVector

pAD-GAL4-2.1 Vector

Prokaryotic Expression

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Eukaryotic Expression

Mammal
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