( Fast imaging and software flex...)Detect Released Oligosaccharides Using the Eagle Eye II,,,Still Video Imaging System

Fast imaging and software flexibility

Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System

Angela R. Downs Deborah A. Greer Stan Ivey
Department of Biology, Delaware State University, Dover, DE

We compared the Eagle Eye II still video system and the Glyko FACE SE2000 gel imager system for imaging Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) gels. The data generated from either system were not significantly different, indicating that successful FACE gel analysis does not require a dedicated single-use device (such as the Glyko SE2000). Consequently, the versatile Eagle Eye II still video system is superior because it can be used for many applications without compromising experimental results.

Glycoprotein carbohydrates play important roles in the biological processes of glycosylated proteins.¹ However, analyzing glycan moieties can be an arduous process, given the small recovery amounts, impurity, and low molecular mass of released glycans. In a recent advance, gel electrophoresis has been used to separate fluorophore-labeled oligosaccharides (FACE) released from glycoproteins using endoglycosidases.²

Following electrophoresis, CCD cameras are widely used for all types of fluorescent or colorimetric gel documentation. The Eagle Eye II still video system is designed to visualize, document, and provide analytical tools for any type of DNA or protein gel. In contrast, the Glyko SE2000 gel imager is designed to image only FACE gels.

We compared a FACE gel imaged first using Stratagenes Eagle Eye II still video imaging system (with a SYBR Green band-pass filter), then imaged the gel again using Glykos SE2000 gel imager (Figure 1). We then evaluated the time required
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