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Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance

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Stratagene introduces a series of control RT-PCR for human gene transcripts of differing abundance. Because they are easy to use and generate different types of information, these primers offer an improvement over RT-PCR tools currently available for analyzing cDNA quality.

Table 1: Human Gene Targets Indicating Abundance Level and Expected Size of cDNA Fragment

Gene

Abundance Level1

PCR product sizeRNA (bp)

b-actin

high

275

g-actin

medium

449

Protein phosphatase 1

low

394

ARF1

low

336

ARF3

low

231

Ornithine decarboxylase

low

381

REFERENCES
  1. Adams M.D., et. al. (1995) Nature 377 (Supplement): 3-174.
  2. Liang, P. and Pardee, A.B. (1992) Science 257: 967-971.
  3. Velculescu, V.E., et al. (1995) Science 270: 484.
  4. Bishop, J.O., et al. (1974) Nature 250: 199-204.
  5. Getz, M.J,. et. al (1975) Cell 4: 121-129.
  6. Heid, C.A., et al. (1996) Genome Research 6: 986-994.
  7. Gibson, U.E.M., Heid, C.A., and P.M. Williams (1996) Genome Research 6: 995-1001.

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