( and product size (0.2 kb12.5 ...)cMaster RTplusPCR System for increased sensitivity and product length ,,, in RT-PCR

and product size (0.2 kb12.5 kb). The cMaster RTplusPCR enzyme mix uses a blend of thermostable DNA polymerases with proofreading assisted fidelity and high extension rate. Performing the One Step procedure, sensitive detection out of smallest amounts of total RNA and amplification of up to 5.3 kb cDNAs is possible (see Fig. 1 and Fig. 2). The Two Step protocol enables multiple cDNA targets to be amplified from an RT reaction, extending the PCR product size up to 12.3 kb (see Fig. 3). To further extend flexibility, the RT-only option of the system can be used to combine cMaster RT with any PCR system or to generate cDNA probes for other downstream applications, e.g. hybridization experiments.





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