Navigation Links
cMaster RTplusPCR System for increased sensitivity and product length ,,, in RT-PCR

ions in 1:10 diluted RTplusPCR Buffer at 2.5 mM final Magnesium concentration.

Set-up for One Step RT-PCR reactions



Cycling program parameters



Experiment 2: One Step, Long Range

Target for RT-PCR amplification: human Tuberous Sclerosis Factor (hTSF) mRNA, a 5.3 kb fragment. Template RNA: total RNA purified from human HELA cells, template concentrations in the reactions are 100 and 10 ng.
Protocol: Eppendorf cMaster RTplusPCR Kit, special One Step
RT-PCR protocol for long templates.

Set-up for One Step RT-PCR reactions



Cycling program parameters



Experiment 3: Two Step RT-PCR

Targets for Two Step RT-PCR amplification:

Alpha Tubulin mRNA (mouse and human), 500 bp fragment
Tumor Necrosis Factor Receptor (TNFR1) mRNA (mouse and human), 1.3 kb fragment
Human Tuberous Sclerosis Factor (hTSF) mRNA, 5.3 kb fragment
Mouse Dynein mRNA, 12.3 kb fragment
Protocol: Eppendorf cMaster RTplusPCR Kit, Two Step RT-PCR
protocol for standard sized and long templates (manual). All reverse
transcription reactions were primed with 0.5 g Oligo(dT)20 primer.


Set-up for the first step RT reaction



Set-up for the second step PCR reaction



Set-up for RT reaction



Two step cycling program used for Tubulin and TNFR1

'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
4. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Enhanced PCR Cloning System
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
10. Yeast Protein Production System Features High Yields and One-Step Purification
11. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
Post Your Comments:
(Date:7/25/2014)... A new study by researchers from the University ... tiny nanosystems function, unlocking the potential to create ... study, which has been published in the prestigious ... a novel laser technique to examine in rich ... a small cluster containing an acetylene molecule and ...
(Date:7/25/2014)... CA (PRWEB) July 25, 2014 WABC Radio ... Your Pet with Lorry Young will be hosting California-based ... talk about stem cell therapy in pets. Dr. Harman ... benefits of stem cell therapy for pets suffering from osteoarthritis ... the Regenerative Veterinary Medicine industry forward. , Young has invited ...
(Date:7/25/2014)... July 25, 2014  Galmed Pharmaceuticals Ltd. (Nasdaq: ... on the development and commercialization of a once-daily, oral ... gallstones, today announced financial results for the period ended ... Summary: , Reported cash and cash equivalents totaling ... at December 31, 2013.  , Reported a net ...
(Date:7/25/2014)... Biotech Ltd. (Nasdaq: SVA ), a leading provider ... announced that it will release its unaudited financial results for ... on Thursday, August 14, 2014 EDT. The Company will host ... August 15, 2014, at 8:00 a.m. EDT (Friday, August 15, ... Company,s financial results and provide an update on recent corporate ...
Breaking Biology Technology:Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 2Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 3Robert J. Harman, DVM, Founder and CEO of Vet-Stem, Inc. to Join New York Radio Host Lorry Young in Upcoming Episode of A “Paws” For Your Pet 2Robert J. Harman, DVM, Founder and CEO of Vet-Stem, Inc. to Join New York Radio Host Lorry Young in Upcoming Episode of A “Paws” For Your Pet 3Galmed Pharmaceuticals Reports First Half 2014 Financial Results 2Galmed Pharmaceuticals Reports First Half 2014 Financial Results 3Galmed Pharmaceuticals Reports First Half 2014 Financial Results 4Galmed Pharmaceuticals Reports First Half 2014 Financial Results 5Galmed Pharmaceuticals Reports First Half 2014 Financial Results 6Sinovac to Host Conference Call to Report Second Quarter 2014 Unaudited Financial Results 2
... Materials Research Laboratory at UC Santa Barbara: an NSF ... from the National Science Foundation (NSF) to carry out ... years. Renewed NSF support ensures the vitality of materials ... energy, environment, and sustainability. Despite the challenging budgetary ...
... Sept. 12, 2011 McKesson Corporation ... McKesson Specialty Health , focused on empowering the ... technology and quality of care. (Logo:   ... support and business solutions, McKesson Specialty Health offers the ...
... Sept. 9, 2011 Palatin Technologies, Inc. (NYSE Amex: ... the Rodman & Renshaw Annual Global Investment Conference on Tuesday, ... will be held at The Waldorf Astoria in New York, ... Executive Officer of Palatin Technologies, will provide an update on ...
Cached Biology Technology:Major grant from the National Science Foundation will sustain UCSB materials research through 2017 2Major grant from the National Science Foundation will sustain UCSB materials research through 2017 3McKesson Corporation Unveils New Offerings to Empower the Community Patient Care Delivery System 2McKesson Corporation Unveils New Offerings to Empower the Community Patient Care Delivery System 3
(Date:7/25/2014)... In the first broad-scale estimate of air pollution removal ... calculated that trees are saving more than 850 human ... respiratory symptoms. , While trees, pollution removal equated to ... percent, the impacts of that improvement are substantial. Researchers ... pollution at nearly $7 billion every year in a ...
(Date:7/25/2014)... the colonial era, 100,000s of people lived on the ... manipulated fire to control the availability of plants they ... continue to use fire to maintain desired habitat and ... U.S. Forest Service,s Pacific Southwest Station, will lead a ... Wildfire Refuge during the Ecological Society of America,s 99th ...
(Date:7/25/2014)... Terra satellite show the different areas that have been affected ... The burn scars show up as reddish-brown splotches of color ... the remote parts of Eastern Russia in the Sakha Republic. ... instrument, it is still possible to see the smoke rising ... below show the devastating number of fires that have plagued ...
Breaking Biology News(10 mins):First national study finds trees saving lives, reducing respiratory problems 2Fire ecology manipulation by California native cultures 2Fire ecology manipulation by California native cultures 3
... ON, July 19, 2013 Kidney patients who take calcium ... 22 per cent higher risk of death than those who ... by Women,s College Hospital,s Dr. Sophie Jamal. The study, ... the long-time practice of prescribing calcium to lower phosphate levels ...
... This news release is available in German . ... of text, the brain sends commands to arms and fingers to tap ... transfer this information as electrical pulses that trigger muscles to move. The ... along the spinal cord. For a long time, scientists assumed that ...
... media has expanded to reach an unlikely new target: ... Technology (NIST) have created networks of molecular data similar ... search would allow Facebook users to find all their ... NIST-designed networks could help scientists rapidly sift through enormous ...
Cached Biology News:Calcium linked to increased risk of heart disease and death in patients with kidney disease 2Haste and waste on neuronal pathways 2Haste and waste on neuronal pathways 3Haste and waste on neuronal pathways 4Facebook for molecules 2Facebook for molecules 3
capacity 250 mL...
... This CLS number is ... created to easily match ... showing no availability yet, ... old Sigma-Aldrich number (Z35,347-7) ...
Certified by the Biological Stain Commission. For staining fresh peripheral blood smears and blood parasites....
... Aminopropylsilane is perhaps the ... chemistry for microarray experiments. ... for Microarrays provide a ... for printing PCR products ...
Biology Products: