Navigation Links
cMaster RTplusPCR System for increased sensitivity and product length ,,, in RT-PCR

Abstract

RT-PCR has become an essential method that allows researchers to determine the presence, structure and level of expression of an mRNA transcript in the organism, tissue or cell of interest. The basic protocol involves the synthesis of a cDNA copy of all the mRNA present in the sample, followed by amplification of the gene(s) of interest. Due to buffer incompatibilities, the two steps (RT and PCR) are often performed separately. While there are kits that combine the two steps into one, the limitations on sensitivity, due to the aforementioned buffer incompatibilities, are great. Eppendorf has designed a new kit with novel chemistries that allow for a more robust single-step RT-PCR reaction and even greater sensitivity in Two Step experiments. This paper examines the performance of this new kit.

Introduction

Reverse Transcription followed by PCR* (RT-PCR) is one of the most popular techniques for RNA analysis. One Step RT-PCR is the method of choice if many RNA samples need to be analyzed in parallel; the Two Step protocol is used for difficult targets or multiple target amplification from a single cDNA pool. The new Eppendorf cMaster RTplusPCR System enables the full range of RT-PCR applications. It contains a novel, recombinant reverse transcriptase, and a high fidelity PCR enzyme mix. In addition, the RTplusPCR buffer is formulated to adjust the Mg2+ concentration automatically, so that there is never a need for optimization of this critical component. This is achieved by weakly chelating Mg 2+ ions: when Mg2+ is present in excess, it is bound by the chelating agent, but as it is needed by the reaction (for Taq, or DNA), it is re
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
4. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Enhanced PCR Cloning System
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
10. Yeast Protein Production System Features High Yields and One-Step Purification
11. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
Post Your Comments:
(Date:9/2/2014)... , Sept. 2, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ... $2.1 million. The orders consist of three large odour control ... . "These orders are consistent with the ... Peter Bruijns , President and CEO. "After a difficult ... we are now beginning to see sales growth. Our lower ...
(Date:9/1/2014)... NEW YORK , Sept. 1, 2014 /PRNewswire/ ... report is available in its catalogue: ... Opportunities, 2019 http://www.reportlinker.com/p02350445/India-Solid-Waste-Management-Vehicles-Market-Forecast-and-Opportunities-2019.html ... municipal corporations are the main governing bodies responsible ... management in cities. On an average, around 135,000 ...
(Date:8/31/2014)... 2014 2014 Market Research ... professional and in-depth research report on the ... information, including Polyacrylamide definition, classification, application, and ... overview. This research covers the international market ... global industry analysis covering macroeconomic environment & ...
(Date:8/29/2014)... The global companion diagnostics market ... It is expected to grow at a CAGR ... valued at $1.8 billion in 2013, according to ... , For more information regarding analysis details and ... research report, titled “Companion Diagnostics Market (Breast Cancer, ...
Breaking Biology Technology:India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 2India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 3India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 4India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 5India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 6India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 7India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 8International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 2International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 3Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3
... TUCSON, Ariz., and GILROY, Calif., April 6 The ... College of Medicine and OmegaGenesis, Inc., announced an ... foot ulcers. The agreement includes joint work in ... to provide information and education to medical professionals and ...
... Online, announced today a strategic partnership with Scottsdale Clinical ... oncologists with cutting edge knowledge discovery and collaboration tools ... will enable more access to the latest therapies to ... to find and communicate with each other about difficult ...
... -- Pharmaxis Ltd (AXS: PXS;,Nasdaq: PXSL ) will ... of Wednesday 8th April 2009. Dr Alan Robertson will ... and answer,investor questions. , The teleconference ... April at 6.00pm U.S. East Coast, 3.00pm U.S. West Coast). ...
Cached Biology Technology:The University of Arizona College of Medicine and OmegaGenesis Announce Collaboration Agreement to Address Diabetic Foot Ulcers 2The University of Arizona College of Medicine and OmegaGenesis Announce Collaboration Agreement to Address Diabetic Foot Ulcers 3The University of Arizona College of Medicine and OmegaGenesis Announce Collaboration Agreement to Address Diabetic Foot Ulcers 4MedTrust Online and Scottsdale Clinical Research Institute at Scottsdale Healthcare Announce Collaboration to Provide Cutting Edge Technology to Improve Patient Care 2
(Date:9/1/2014)... of the U.S. diet showed some modest improvement in ... reduction in the consumption of unhealthy trans fats, but ... rich and the poor. , , An unhealthy diet ... cancers. Eating a healthy diet is an important part ... population trends in diet quality is important because it ...
(Date:9/1/2014)... microscopy revealed the presence of tiny blob-like structures that ... still don,t know what they do even though ... be crucial to the life of a cell, and ... In the Journal of Cell Biology , two ... backgrounds, from biophysics to cell biology, to focus their ...
(Date:8/31/2014)... for sniffing the highly infectious bacteria C. diff ... been developed by a team at the University of ... has demonstrated that it is possible to identify the ... lead to rapid diagnosis of the condition., What is ... to identify different strains of the disease simply from ...
Breaking Biology News(10 mins):Quality of US diet improves, gap widens for quality between rich and poor 2Quality of US diet improves, gap widens for quality between rich and poor 3Scientists call for investigation of mysterious cloud-like collections in cells 2Scientists develop 'electronic nose' for rapid detection of C. diff infection 2
... has awarded a $630,100 project support grant to ... the initial implementation this year of a global ... primary care providers, technical staff and health policy-makers. ... Blocks (HIBBs), a program developed by AMIA in ...
... The latest findings from research on Antarctica,s rich marine ... for the Advancement of Science (AAAS). Marine Biologist Huw ... a major international investigation into the distribution and abundance ... Antarctic Marine Life (CAML). Griffiths presents results from ...
... UCSF team, led by bioethicist Bernard Lo, MD, recommends that ... cell research be modified to better protect the rights of ... fertilization. The recommendation is reported in the February 19, ... donate sperm and egg, or "gametes," for patients attempting to ...
Cached Biology News:Rockefeller Foundation supports expansion, training of e-health work force in developing world 2Rockefeller Foundation supports expansion, training of e-health work force in developing world 3Understanding global climate change through new breakthroughs in polar research 2NIH stem cell guidelines should be modified, UCSF team reports 2NIH stem cell guidelines should be modified, UCSF team reports 3
Request Info...
Anti-ZFP200 Family: Zinc Finger Peptide Sequence: CGKSFNHKTNLNKHER...
Donor feline serum and plasma...
Collected from sexually mature, mixed sex, mixed breed cats. Physiochemical constituents are measured to ensure lot-to-lot consistency....
Biology Products: