Navigation Links
cDNA Sequencing & Finishing


Agencourt offers sequencing services designed specifically for clients who require either EST or full length cDNA sequencing and finishing. Agencourt has many features that allow us to provide industry-leading sequence quality with rapid project turnaround including:

Comprehensive genomic pipeline
High throughput, GLP-compliant facility
Patented SPRI technology
Proven experience with large cDNA sequencing projects


FULL SERVICE PROVIDER
Agencourt has a fully-automated genomic services pipeline based on SPRI, our patented magnetic bead-based nucleic acid purification technology. The high throughput nature of our pipeline allows for rapid 5 cDNA screening. Key cDNA clones identified during screening are easily integrated into our pipeline for high quality full length sequencing and finishing. All aspects of the pipeline are integrated and monitored by our Galaxy LIMS (Laboratory Information Management System) to ensure data quality.


CDNA SEQUENCING SERVICE
Our cDNA sequencing services include:
Template purification
Sequence reaction set up and thermocycling
Dye-terminator removal
Sequence delineation on an ABI PRISM 3700/3730xl
cDNA assembly and finishing for full length cDNA projects
Data delivery via a secure FTP site


SEQUENCING AND FINISHING
Purified cDNA clones are sequenced using BigDye Terminator v3.1 chemistry and reactions purified using CleanSEQ, a SPRI-based dye-terminator removal system. Typical Phred20 read lengths are greater than 550bp and pass rates greater than 85%. cDNA sequences are typical ly finished using primer walking. If a reference sequence is known, unique primer walking strategies allow for sample turnaround in less than two weeks.


MGC PROJECT
The Mammalian Gene Collection (MGC) is an NIH-funded program aimed at identifying and sequencing cDNA clones containing a full-length open reading frame for all human and mouse genes. Agencourt was selected as the provider for all 5 EST sequencing of MGC cDNA libraries. Table 1 shows sequencing performance for over 15,000 EST reads conducted as part of the MGC initiative. Agencourt has submitted over 800,000 high quality cDNA sequences to GenBank as part of the MGC initiative.


FOR MORE INFORMATION
Please contact us at: 800-361-7780 or
email us at: info@agencourt.com


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. Eppendorf Mastercycler ABI Big Dye Sequencing Protocol
4. QuickLane 24 - 48 Hour Sequencing
5. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
6. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
7. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
8. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/26/2016)... , May 26, 2016 Despite the ... value in this space. Today,s pre-market research on ActiveWallSt.com directs ... Health Inc. (NASDAQ: RDUS ), Cerus Corp. (NASDAQ: ... ARWR ), and Five Prime Therapeutics Inc. (NASDAQ: ... briefings at: http://www.activewallst.com/ On ...
(Date:5/26/2016)... San Diego, CA (PRWEB) , ... May 26, ... ... assay development and manufacturing company, today announced several positive developments that position the ... As a result of the transaction, Craig F. Kinghorn has been appointed ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lajollacooks4u has become a rising ... been rated one of its top attractions. Fortune 500 companies, such as Illumina, ... a unique and intimate team-building experience. , Each event kicks off with an olive ...
(Date:5/25/2016)... ... May 25, 2016 , ... The Ankle Plating ... options designed to address fractures of the distal tibia and fibula. This system ... Ankle Plating System 3 is composed of seven plate families that span the ...
Breaking Biology Technology:
(Date:3/15/2016)... -- Yissum Research Development Company of the Hebrew ... Hebrew University, announced today the formation of Neteera ... human biological indicators. Neteera Technologies has completed its first ... ... emissions from sweat ducts, enables reliable and speedy biometric ...
(Date:3/14/2016)... , Allemagne, March 14, 2016 ... http://www.apimages.com ) - --> - Renvoi : ... - --> --> ... solutions biométriques, fournit de nouveaux lecteurs d,empreintes digitales ... LF10 de DERMALOG sera utilisé pour produire des ...
(Date:3/11/2016)... India , March 11, 2016 ... a new market research report "Image Recognition Market by ... Application (Marketing and Advertising), by Deployment Type (On-Premises and ... Forecast To 2022", published by MarketsandMarkets, the global market ... 2015 to USD 29.98 Billion by 2020, at a ...
Breaking Biology News(10 mins):