Navigation Links
cDNA Sequencing & Finishing


Agencourt offers sequencing services designed specifically for clients who require either EST or full length cDNA sequencing and finishing. Agencourt has many features that allow us to provide industry-leading sequence quality with rapid project turnaround including:

Comprehensive genomic pipeline
High throughput, GLP-compliant facility
Patented SPRI technology
Proven experience with large cDNA sequencing projects


FULL SERVICE PROVIDER
Agencourt has a fully-automated genomic services pipeline based on SPRI, our patented magnetic bead-based nucleic acid purification technology. The high throughput nature of our pipeline allows for rapid 5 cDNA screening. Key cDNA clones identified during screening are easily integrated into our pipeline for high quality full length sequencing and finishing. All aspects of the pipeline are integrated and monitored by our Galaxy LIMS (Laboratory Information Management System) to ensure data quality.


CDNA SEQUENCING SERVICE
Our cDNA sequencing services include:
Template purification
Sequence reaction set up and thermocycling
Dye-terminator removal
Sequence delineation on an ABI PRISM 3700/3730xl
cDNA assembly and finishing for full length cDNA projects
Data delivery via a secure FTP site


SEQUENCING AND FINISHING
Purified cDNA clones are sequenced using BigDye Terminator v3.1 chemistry and reactions purified using CleanSEQ, a SPRI-based dye-terminator removal system. Typical Phred20 read lengths are greater than 550bp and pass rates greater than 85%. cDNA sequences are typical
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. Eppendorf Mastercycler ABI Big Dye Sequencing Protocol
4. QuickLane 24 - 48 Hour Sequencing
5. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
6. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
7. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
8. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: cDNA Sequencing Finishing

(Date:7/11/2014)...  Researchers have pioneered a revolutionary new way ... called Virtual Finger, allows scientists to move through ... synapses using the flat surface of their computer ... studies orders of magnitude more efficient, saving time, ... many areas of experimental biology. The software and ...
(Date:7/10/2014)... 2014 Understanding the need for better ... and the gap that currently exists in this area, ... industry-leading portfolio of validated ion channel and GPCR cell ... lines, as ion channels control many critical physiological functions ... creates the potential to treat a variety of human ...
(Date:7/10/2014)... EvoDerma ’s NOOME Anti-Aging Motion has been ... comes with a second treatment cup to help prevent and ... cup is thinner on the edges for a softer contact ... and uneven surfaces on the face, neck and décolleté. Choose ... on areas such as the cheeks and neck, or opt ...
(Date:7/10/2014)... PROVO, Utah , July 10, 2014 ... genome annotation and interpretation, today announced an ... sequencing (NGS) analytics for Lineagen,s NextStep Dx ... evaluation of neurodevelopmental and neurological disorders, currently ... chromosomal microarray (CMA) testing service used by ...
Breaking Biology Technology:Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 3Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4
... Devgen,(Eurolist: DEVG) and Monsanto Company (NYSE: MON ... which Monsanto has agreed, subject to certain,customary conditions, to ... 18 million (25 million $) and representing 5.9%,of the ... take place,no later than November 2007. It is ...
... announces today,that a service agreement with Roche Diagnostics ... its automated immunohistochemistry,service platform for analyzing various biomarkers ... will provide for this project a selection of,tumor ... as specifically,collected clinical samples. The analysis of specific ...
... Nationally, SCOTTSDALE, Ariz., Sept. 14 CHS, an ... by the U.S. Department,of Defense as a recipient of ... award, sponsored by the National Committee of Employer,Support of ... honor,bestowed upon America,s employers who provide support to their ...
Cached Biology Technology:Monsanto Company Signs Share Subscription Agreement With Devgen 2Monsanto Company Signs Share Subscription Agreement With Devgen 3CHS Receives Prestigious Honor from Department of Defense 2CHS Receives Prestigious Honor from Department of Defense 3
(Date:7/10/2014)... 27, 2014  The American Academy of Forensic ... Standards and Technology (NIST) and the Department of ... and forensic science expert members to the Forensic ... element of the NIST,s Organization of Scientific Area ... first appointments made to the new FSSB that ...
(Date:7/10/2014)... 1, 2014 Smart technology emphasis grows ... and improved security: NXT-ID, Inc. (OTCBB: NXTD), eBay Inc. (NASDAQ: ... ), Apple Inc. (NASDAQ: AAPL ), Visa Inc. ... NXT-ID, Inc., (OTCQB: NXTD) a biometric authentication company focused ... the second series of 30 second spots on CNBC for ...
(Date:7/10/2014)... July 2, 2014 NXT-ID, Inc., (OTCQB: NXTD) a biometric authentication ... Mr. Gino Pereira was interviewed on July 1 ... Tampa Florida . Mr. Pereira discusses the company,s ... Harris and how the Wocket™ aims to replace a ... Cedric Harris tells Gino he has never ...
Breaking Biology News(10 mins):Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4
... years for marine invertebrates living on the Port Valdez ... according to a scientist at the University of Alaska ... Richter scale, and the tsunami waves that followed, impacted ... Blanchard, a research assistant professor at the UAF School ...
... pig population continues to expand, increasing the potential for interaction ... Researchers at North Carolina State University examined feral pigs from ... can be transmitted from animals to people Toxoplasma ... study found that wild pigs host a significant number of ...
... their collaborators have detected the antimatter partner of the ... heaviest antimatter particle ever detected. Scientists at ... detector that identified antihelium-4. The $7.5 million detector was ... Chinese scientists contributing $2.5 million to the project. The ...
Cached Biology News:Port Valdez invertebrates stabilized 26 years after quake 2Wild hogs: Researchers examine impact of feral pigs in eastern N.C. 2Rice-born detector finds heaviest antimatter 2Rice-born detector finds heaviest antimatter 3
... This kit allows for rapid, convenient, and ... simple bind/wash/elute process utilizes a spin column ... beads for concentration of proteins which were ... are strong ion exchangers, offering high binding ...
... angle system, 220-240 V, is used to resolve ... fragments by pulsed field gel electrophoresis (PFGE). The ... pump, 14 x 13 cm casting stand with ... thick comb, screened cap, disposable plug molds, 12 ...
... kit is designed to knockout or alter genes ... than one week. Red/ET recombination allows the exchange ... specific, and faithful manner. An FRT-flanked kanamycin resistance ... can be used to replace a gene on ...
... separation system using the asymmetric flow Field ... easily with Wyatts multi-angle light scattering (MALS) ... the Optilab DSP. The combined system provides ... the particles/molecules separated by the AFFF unit. ...
Biology Products: