Navigation Links
cDNA Sequencing & Finishing


Agencourt offers sequencing services designed specifically for clients who require either EST or full length cDNA sequencing and finishing. Agencourt has many features that allow us to provide industry-leading sequence quality with rapid project turnaround including:

Comprehensive genomic pipeline
High throughput, GLP-compliant facility
Patented SPRI technology
Proven experience with large cDNA sequencing projects


FULL SERVICE PROVIDER
Agencourt has a fully-automated genomic services pipeline based on SPRI, our patented magnetic bead-based nucleic acid purification technology. The high throughput nature of our pipeline allows for rapid 5 cDNA screening. Key cDNA clones identified during screening are easily integrated into our pipeline for high quality full length sequencing and finishing. All aspects of the pipeline are integrated and monitored by our Galaxy LIMS (Laboratory Information Management System) to ensure data quality.


CDNA SEQUENCING SERVICE
Our cDNA sequencing services include:
Template purification
Sequence reaction set up and thermocycling
Dye-terminator removal
Sequence delineation on an ABI PRISM 3700/3730xl
cDNA assembly and finishing for full length cDNA projects
Data delivery via a secure FTP site


SEQUENCING AND FINISHING
Purified cDNA clones are sequenced using BigDye Terminator v3.1 chemistry and reactions purified using CleanSEQ, a SPRI-based dye-terminator removal system. Typical Phred20 read lengths are greater than 550bp and pass rates greater than 85%. cDNA sequences are typical ly finished using primer walking. If a reference sequence is known, unique primer walking strategies allow for sample turnaround in less than two weeks.


MGC PROJECT
The Mammalian Gene Collection (MGC) is an NIH-funded program aimed at identifying and sequencing cDNA clones containing a full-length open reading frame for all human and mouse genes. Agencourt was selected as the provider for all 5 EST sequencing of MGC cDNA libraries. Table 1 shows sequencing performance for over 15,000 EST reads conducted as part of the MGC initiative. Agencourt has submitted over 800,000 high quality cDNA sequences to GenBank as part of the MGC initiative.


FOR MORE INFORMATION
Please contact us at: 800-361-7780 or
email us at: info@agencourt.com


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. Eppendorf Mastercycler ABI Big Dye Sequencing Protocol
4. QuickLane 24 - 48 Hour Sequencing
5. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
6. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
7. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
8. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/10/2016)... Early-career researchers from ... , Uganda and Yemen ... nutrition   Indonesia , Nepal ... Yemen are being honored for their accomplishments in ... celebrated for mentoring young women scientists who are pursuing careers in agriculture, ...
(Date:2/10/2016)... ASAE is introducing a hybrid membership model which will ... of joining or renewing through an organizational purchasing model. ... every employee in any size association or AMC office ... member benefits.   John H. Graham, IV ... allow organizations of any size and their employees to ...
(Date:2/10/2016)... , Feb. 10, 2016  Allergan plc (NYSE: ... that Brent Saunders , Allergan,s CEO and President, ... fireside chat session at the RBC Capital Markets Healthcare ... ET at The New York Palace Hotel in ... be webcast live and can be accessed on Allergan,s ...
(Date:2/10/2016)... , ... February 10, 2016 , ... ... the International Society of Pharmaceutical Engineering (ISPE) Rocky Mountain Chapter 21st Annual Vendor ... expecting to fill more than 100 tables for its annual event, which will ...
Breaking Biology Technology:
(Date:1/28/2016)... -- Synaptics (NASDAQ: SYNA ), a leading developer of human ... December 31, 2015. --> --> ... 2 percent compared to the comparable quarter last year to $470.5 ... $35.0 million, or $0.93 per diluted share. ... quarter of fiscal 2016 grew 9 percent over the prior year ...
(Date:1/25/2016)... -- Glencoe Software, the world-leading supplier of image data management ... the data management solution OMERO Plus for the newly ... Photo - http://photos.prnewswire.com/prnh/20160125/325328LOGO ... Phenotypic analysis measures the characteristics and behavior of cells, ... such as health and disease, the presence or absence ...
(Date:1/21/2016)... 2016 --> ... research report "Emotion Detection and Recognition Market by Technology (Bio-Sensors, ... Expression, Voice Recognition and Others), Services, Application Areas, ... 2020", published by MarketsandMarkets, the global Emotion Detection ... 22.65 Billion by 2020, at a CAGR of ...
Breaking Biology News(10 mins):