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cDNA Sequencing & Finishing


Agencourt offers sequencing services designed specifically for clients who require either EST or full length cDNA sequencing and finishing. Agencourt has many features that allow us to provide industry-leading sequence quality with rapid project turnaround including:

Comprehensive genomic pipeline
High throughput, GLP-compliant facility
Patented SPRI technology
Proven experience with large cDNA sequencing projects


FULL SERVICE PROVIDER
Agencourt has a fully-automated genomic services pipeline based on SPRI, our patented magnetic bead-based nucleic acid purification technology. The high throughput nature of our pipeline allows for rapid 5 cDNA screening. Key cDNA clones identified during screening are easily integrated into our pipeline for high quality full length sequencing and finishing. All aspects of the pipeline are integrated and monitored by our Galaxy LIMS (Laboratory Information Management System) to ensure data quality.


CDNA SEQUENCING SERVICE
Our cDNA sequencing services include:
Template purification
Sequence reaction set up and thermocycling
Dye-terminator removal
Sequence delineation on an ABI PRISM 3700/3730xl
cDNA assembly and finishing for full length cDNA projects
Data delivery via a secure FTP site


SEQUENCING AND FINISHING
Purified cDNA clones are sequenced using BigDye Terminator v3.1 chemistry and reactions purified using CleanSEQ, a SPRI-based dye-terminator removal system. Typical Phred20 read lengths are greater than 550bp and pass rates greater than 85%. cDNA sequences are typically finished using primer walking. If a reference sequence is known, unique primer walking strategies allow for sample turnaround in less than two weeks.


MGC PROJECT
The Mammalian Gene Collection (MGC) is an NIH-funded program aimed at identifying and sequencing cDNA clones containing a full-length open reading frame for all human and mouse genes. Agencourt was selected as the provider for all 5 EST sequencing of MGC cDNA libraries. Table 1 shows sequencing performance for over 15,000 EST reads conducted as part of the MGC initiative. Agencourt has submitted over 800,000 high quality cDNA sequences to GenBank as part of the MGC initiative.


FOR MORE INFORMATION
Please contact us at: 800-361-7780 or
email us at: info@agencourt.com


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