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cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

>Measuring adenylyl cyclase activity: Cultured T-47D cells were treated for 15 min with either buffer (control), forskolin, isoproternol, or isoproterenol and propranolol. Formation of cAMP was determined with the cAMP Assay Kit. The assay was performed in 384-well microplates in a final volume of 40 L. Each treatment group was performed in triplicate.



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1 Chedeville, A. et al. FEBS Letters 319:171176 1993
2 Chan, S.C. and Hanifan, J.M. S. J. Clin. Med. 121:4451 1993


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