Navigation Links
cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

A Sensitive, Robust, Homogenous, and Rapid Assay
Uses High Efficiency Fluorescence Polarization (HEFP) detection method
Detects pmol amounts of cAMP
Optimized for cell extract and enzyme preparations
Requires no separation or wash steps
Available in a kit and in bulk packaging


Introduction
Cyclic adenosine monophosphate (cAMP) is a second messenger involved in the transduction of extracellular signals. The signal is initiated by the binding of a ligand to a G-protein coupled receptor followed by a series of enzyme mediated steps leading to the activation of the enzyme adenylyl cyclase. Adenylyl cyclase converts ATP into cyclic AMP that in turn activates protein kinases. Protein kinase activity is correlated to the intracellular concentration of cyclic AMP. The level of cyclic AMP is reduced by phosphodiesterase. Assays that determine the activities of these cellular regulators are important tools for high-throughput screening laboratories.


Intended Use
The cAMP Assay Kit is designed to determine concentrations of cyclic AMP in solution. The sources of cyclic AMP analyte may either be in vitro enzyme preparations or cell extracts.


Principle of the Assay
The assay is an antibody-mediated competitive binding reaction with a High Efficiency Fluorescence Polarization (HEFP) read out. Cyclic AMP (analyte) from sample, calibrators, or controls competes with fluorescein-labeled cAMP (tracer) for a limited number of anti-cAMP antibodies. In the absence of analyte, a maximal amount of tracer will be bound to the antibody.

Addition of analyte competes with the tracer for the limited binding sites, so that the extent of polarization is inversely proportional to the concentration of analyte.

HEFP is a detection method that discriminates on th
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
2. Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system
3. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
4. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
5. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
6. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
7. Mutation detection for the K- rasand P16 genes
8. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
9. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
10. Front-face detection for high-concentrate, opaque or solid sample
11. Outstanding transfection rates using the Multiporator
Post Your Comments:
(Date:3/2/2015)... British Columbia , March 2, 2015 ... IMLFF), a clinical stage biopharmaceutical company specializing in ... that Craig Schneider , Chief Executive Officer, ... webcast at VirtualInvestorConferences.com. DATE: Thursday, March ... > click the red "register/ watch event now" ...
(Date:3/2/2015)... -- Editor Note: For more information about ... Moments ago, Analysts Review released new research updates concerning several ... SSH ), Synergetics USA (NASDAQ: ... ), Spectranetics (NASDAQ: SPNC ), and Thoratec (NASDAQ: ... platform for investors, to hear about what matters - proudly ...
(Date:3/2/2015)... Kent, WA (PRWEB) March 02, 2015 ... tangential flow flat-sheet testing system at the 2015 AMTA ... is a culmination of a decade’s worth of engineering ... system compatible with a wide range of applications across ... be versatile enough to meet the dynamic needs of ...
(Date:3/1/2015)... 2015 Market Publishers Ltd and ... market research promotion on Internet. MarketPublishers.com is now ... out by DataGroup Booksellers. , Natalie Aster, Assistant ... commented on the recent partnership agreement as follows: ... our team of publishers. Since 1974, the company ...
Breaking Biology Technology:InMed Pharmaceuticals to Present Investor Webcast March 5 2Quarterly Report Card of Biotechnology Companies: New Research on Sunshine Heart, Synergetics USA, Syneron Medical, Spectranetics and Thoratec 2Quarterly Report Card of Biotechnology Companies: New Research on Sunshine Heart, Synergetics USA, Syneron Medical, Spectranetics and Thoratec 3Quarterly Report Card of Biotechnology Companies: New Research on Sunshine Heart, Synergetics USA, Syneron Medical, Spectranetics and Thoratec 4Sterlitech Displays Complete CF042 Membrane Test System 2Market Publishers Ltd and DataGroup Booksellers Sign Partnership Agreement 2Market Publishers Ltd and DataGroup Booksellers Sign Partnership Agreement 3
... recommend Corvalen(R) D-Ribose to boost cellular energy, NEW YORK, ... you,could sleep all year and not catch up? That,s how ... there was a good reason to be,exhausted -- five boys ... fix., Then came a sixth son, and she began ...
... System Combines Clinical Benefits of Dual Wavelengths Through ... Time While Providing a Uniform, Safe and Effective ... Treatment - New Offering Signals Company,s Expansion into International Laser ... Lipolysis Market, WESTFORD, Mass., April 28 Cynosure, ...
... Calif., April 28 Aida,Pharmaceuticals, Inc. (OTC Bulletin Board: ... its Phase 2 testing results of Rh-Apo2L and,expects to ... announced that the target cancers for the drug have ... targets,that the Company has chosen are ailments which Rh-Apo2L ...
Cached Biology Technology:D-Ribose Helps Energy-Starved Hearts 2Cynosure Introduces Smartlipo MPX(TM) Dual Wavelength Workstation for LaserBodySculpting(SM) 2Cynosure Introduces Smartlipo MPX(TM) Dual Wavelength Workstation for LaserBodySculpting(SM) 3Cynosure Introduces Smartlipo MPX(TM) Dual Wavelength Workstation for LaserBodySculpting(SM) 4Cynosure Introduces Smartlipo MPX(TM) Dual Wavelength Workstation for LaserBodySculpting(SM) 5Aida Pharmaceuticals, Inc. Announces Update on Progress of Rh-Apo2L Testing 2
(Date:2/5/2015)... , Jan. 27, 2015   Marvin ... globally deployed, innovative test solutions for military, ... delivery of its TS-323 GENASYS Test ... Company (LMSSC). GENASYS is a high-performance PXI-based ... mission-critical applications that require performance functional testing. ...
(Date:2/5/2015)... 2015 Despite a lack of advancements ... as consumer electronics, automotive, storage and telecommunication has ... (SMT) screen printers. Innovations in electronics and conversion ... the adoption curve up. Meanwhile, sale volumes of ... dispensers are indispensable in the production of complex ...
(Date:1/22/2015)... Hall E -   EyeLock Inc. , a market leader ... its EyeLock ID technology integrated in a 3D printed automobile ... Ridge National Laboratory (ORNL) at the 2015 North ... is being used to validate the driver and authorize the ...
Breaking Biology News(10 mins):Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 2Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 3Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 4End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3
... more than 300 leading European and Chinese scientists have ... in the Peoples Republic of China to present their ... Dragon 2. The Dragon Programme is a joint ... Technology (MOST) of China designed to encourage increased exploitation ...
... 24, 2008) The Wildlife Conservation Society (WCS) recently ... is affecting populations of a quintessential Arctic denizen: the ... National Park Service, U. S. Geological Survey, and Alaska ... equipped six musk ox with GPS collars to better ...
... Addiction and Mental Health (CAMH) has discovered a ... (MR) along with the eye defect retinitis pigmentosa ... that causes this disorder, CC2D2A. This scientific ... processes involved in brain development, and may help ...
Cached Biology News:Successful cooperation extends Dragon Program 2Successful cooperation extends Dragon Program 3New gene discovered for new form of intellectual disability 2
Polymerase (RNA) II (DNA directed) polypeptide L...
... PI is used in conjuction with Annexin ... the earlier stages of apoptosis (Annexin V ... in later stages of apoptosis or already ... is detected in the orange range of ...
BD Primaria 35 mm Cell Culture Dish with Easy-Grip design, surface-modified polystyrene for enhanced cell culture. (20/sp, 200/ca) Packaging: 20 / sleeve, 500 / case ...
... DNA Polymerase is a premium quality recombinant ... thermostable enzyme is suitable for a wide ... highest purity and reproducible performance, each preparation ... quality control assays. NovaTaq DNA Polymerase possesses ...
Biology Products: