Navigation Links
cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

A Sensitive, Robust, Homogenous, and Rapid Assay
Uses High Efficiency Fluorescence Polarization (HEFP) detection method
Detects pmol amounts of cAMP
Optimized for cell extract and enzyme preparations
Requires no separation or wash steps
Available in a kit and in bulk packaging


Introduction
Cyclic adenosine monophosphate (cAMP) is a second messenger involved in the transduction of extracellular signals. The signal is initiated by the binding of a ligand to a G-protein coupled receptor followed by a series of enzyme mediated steps leading to the activation of the enzyme adenylyl cyclase. Adenylyl cyclase converts ATP into cyclic AMP that in turn activates protein kinases. Protein kinase activity is correlated to the intracellular concentration of cyclic AMP. The level of cyclic AMP is reduced by phosphodiesterase. Assays that determine the activities of these cellular regulators are important tools for high-throughput screening laboratories.


Intended Use
The cAMP Assay Kit is designed to determine concentrations of cyclic AMP in solution. The sources of cyclic AMP analyte may either be in vitro enzyme preparations or cell extracts.


Principle of the Assay
The assay is an antibody-mediated competitive binding reaction with a High Efficiency Fluorescence Polarization (HEFP) read out. Cyclic AMP (analyte) from sample, calibrators, or controls competes with fluorescein-labeled cAMP (tracer) for a limited number of anti-cAMP antibodies. In the absence of analyte, a maximal amount of tracer will be bound to the antibody.

Addition of analyte competes with the tracer for the limited binding sites, so that the extent of polarization is inversely proportional to the concentration of analyte.

HEFP is a detection method that discriminates on th
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
2. Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system
3. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
4. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
5. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
6. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
7. Mutation detection for the K- rasand P16 genes
8. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
9. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
10. Front-face detection for high-concentrate, opaque or solid sample
11. Outstanding transfection rates using the Multiporator
Post Your Comments:
(Date:8/20/2014)... (PRWEB) August 20, 2014 ... released today including improvements to the enterprise replication ... connectors for Lucene, SOLR and Elasticsearch. This release ... this high performance triplestore – GraphDB™ was formerly ... in deploying the only mature enterprise resilient RDF ...
(Date:8/19/2014)... Columbia, Md. (PRWEB) August 19, 2014 ... integrated liquid chromatography systems, Prominence-i and Nexera-i, adding ... UHPLC systems. Combining excellent functionality, an intuitive operating ... performance and a more efficient workflow for conventional ... systems feature innovative, intuitive and intelligent design so ...
(Date:8/19/2014)... DANVILLE, Pa. , Aug. 19, 2014 ... C has been nominated for a Centers for Disease ... to addressing a major public health concern. ... Health System, is one of a team of researchers ... (CDC) Charles C. Shepard Science Award for Data Methods ...
(Date:8/19/2014)... -- Research and Markets  has announced the addition of ... Business Report" report to their offering. ... Cells (SOFCs) in US$ Thousands. The report provides separate comprehensive ... Europe , Asia-Pacific , and ... the period 2012 through 2020. Market data and analytics are ...
Breaking Biology Technology:Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 3Geisinger investigator nominated for federal award 2Geisinger investigator nominated for federal award 3Global Solid Oxide Fuel Cells (SOFCs) - Strategic Business Report 2014 2
... RedPrairie Corporation , a ... that enables business process transformation, ... of the top 20 retailers ... Performance Management to optimize their ...
... Centers for Medicare and Medicaid Services ("CMS") and ... General ("OIG") published separate, but parallel, proposed rules ... of widespread adoption of electronic health records technologies ... These proposed rules are in accordance with the ...
... A new report suggests Wisconsin is slowly making progress when ... indicators such as producing patents, investing in private research and ... we improving? , ,There is no single reason, but ... not swamped in a sea of global competition. , ...
Cached Biology Technology:The use of e-prescribing and electronic health records is accelerating 2The use of e-prescribing and electronic health records is accelerating 3The use of e-prescribing and electronic health records is accelerating 4The use of e-prescribing and electronic health records is accelerating 5Heres why Wisconsins tech economy indicators are up 2Heres why Wisconsins tech economy indicators are up 3
(Date:8/20/2014)... acral melanomas the rare type of skin cancer that ... from other more common types of skin cancer, according to ... Pigment Cell & Melanoma Research . , Acral melanoma most ... feet, nail-beds and other hairless parts of the skin. Unlike ... UV damage from the sun. , The team, from the ...
(Date:8/20/2014)... chairs, synthetic rugs and plastic bags could one day ... rather than petroleum, scientists are now reporting. The novel ... the world deal with its agricultural and plastic waste ... , Athanassia Athanassiou, Ilker S. Bayer and colleagues at ... popularity is constantly growing. In 2012, its production reached ...
(Date:8/20/2014)... absorbs light and its energy is finally utilized ... Photovoltaic devices, mostly build from silicon based semiconductors, ... approach for the development of cheaper and renewable ... isolated membrane protein complexes of photosynthesis. Prof. Dr. ... from thermophilic cyanobacteria that live in a hot ...
Breaking Biology News(10 mins):Scientists learn more about rare skin cancer that killed Bob Marley 2A semi-artificial leaf faster than 'natural' photosynthesis 2
... showed that a fruit fly gene is crucial for ... how the transformation initially proceeds. Understanding this process in ... teenagers. , When the DHR4 gene is disabled, fruit ... to a sexually active adult. It is the first ...
... new surgical assistant at the University of North Carolina ... brain and a glowing track record in helping to ... arteries and perform gastric bypass operations for morbid obesity. ... da Vinci Surgical System, manufactured by Intuitive Surgical. UNC ...
... the link between two biochemical pathways that plants use ... long believed worked independently of each other. , Knowing ... help researchers breed plants that can better resist a ... author and an assistant professor of horticulture and crop ...
Cached Biology News:MWG Biotech expands siMAX?siRNA portfolio with new scales, lengths and design tools 2MWG Biotech expands siMAX?siRNA portfolio with new scales, lengths and design tools 3MWG Biotech expands siMAX?siRNA portfolio with new scales, lengths and design tools 4Screen all at risk for HIV, plus pregnant women 2Screen all at risk for HIV, plus pregnant women 3Study: Plants use dual defense system to fight pathogens 2
... Our fastest automated perfusion system. ... in perfusion, physiology, biophysics, electrochemistry, or ... reproducibility with 1.5-4 millisecond accuracy ... Microprocessor-based for accuracy and flexibility ...
Recombinant Human PTP1B (aa 2-321), CF...
... This DuoSet ELISA Development kit contains ... of sandwich ELISAs to measure natural and ... supernates and serum. Each kit contains sufficient ... 96-well plates, provided that the following conditions ...
FGF Receptor 1 Antibody...
Biology Products: