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aRNA Synthesis and Labeling for Array Analysis

Demonstrating Equivalent Labeling. Scatter plot showing signal intensities of Cy3 vs. Cy5 labeled aRNA synthesized with the Amino Allyl MessageAmp II aRNA Kit.


Now Available with Cy dyes

For versatility and convenience, the new Amino Allyl MessageAmp II aRNA Kit can be ordered with Cy3, Cy5, Cy3 and Cy5, or without label. Other nonisotopic labels, available from Amersham Biosciences, Molecular Probes, and Pierce, can also be coupled to the amino allyl moiety.


Improved Kit for High Yield and High Reproducibility

The Amino Allyl MessageAmp aRNA Amplification Kit was the first kit that integrated amino allyl incorporation with RNA amplification. The new Amino Allyl MessageAmp II aRNA Kit includes extensive improvements to the original kit. Both kits contain all the components needed to perform RNA amplification, including reagents for first- and second-strand cDNA synthesis, cDNA purification, large scale in vitro transcription, and aRNA purification. Additionally, both kits include Ambion's highly purified amino allyl UTP, and reagents for Cy dye coupling and clean-up. The final purification step has been optimized for thorough removal of excess dye to ensure very low background signal on arrays.

The Amino Allyl MessageAmp II aRNA Amplification Kit uses ArrayScript to maximize conversion of first-strand cDNA into full-length double-stranded cDNA templates [1]. Buffers have been modi
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