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aRNA Synthesis and Labeling for Array Analysis

Amino Allyl MessageAmp II aRNA Amplification Kits-- Now Available with Cy Dyes
Extremely sensitive
ArrayScript Reverse Transcriptase--high yields of full-length cDNA increases aRNA yields

MEGAscript transcription technology-- up to 4000-fold amplification of mRNA

Optimized buffers--high dye coupling efficiency


Maximum flexibility
Configured for 20 single round amplifications or 10 two round amplifications

Available with Cy3, Cy5, both Cy3 & Cy5, or without dyes

Sufficient aRNA for most microarray analyses from a four hour in vitro transcription reaction (>500 ng input RNA)


Labeling aRNA

Several different methods can be used to label amplified antisense RNA (aRNA) for array analysis. Cy dyes, other fluorophores, or biotin can be incorporated: 1) during the in vitro transcription (IVT) step of aRNA synthesis via modified-rNTP incorporation (direct labeling), 2) during a subsequent reverse transcription reaction via modified-dNTP incorporation (direct labeling), or 3) in a coupling reaction after aRNA synthesis where the label is added to an amino allyl-modified rNTP incorporated during IVT or reverse transcription (indirect labeling). Increasingly, amino allyl incorporation combined with a secondary dye coupling reaction is being used for the generation of samples for array
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