Navigation Links
aRNA Synthesis and Labeling for Array Analysis

Amino Allyl MessageAmp II aRNA Amplification Kits-- Now Available with Cy Dyes
Extremely sensitive
ArrayScript Reverse Transcriptase--high yields of full-length cDNA increases aRNA yields

MEGAscript transcription technology-- up to 4000-fold amplification of mRNA

Optimized buffers--high dye coupling efficiency


Maximum flexibility
Configured for 20 single round amplifications or 10 two round amplifications

Available with Cy3, Cy5, both Cy3 & Cy5, or without dyes

Sufficient aRNA for most microarray analyses from a four hour in vitro transcription reaction (>500 ng input RNA)


Labeling aRNA

Several different methods can be used to label amplified antisense RNA (aRNA) for array analysis. Cy dyes, other fluorophores, or biotin can be incorporated: 1) during the in vitro transcription (IVT) step of aRNA synthesis via modified-rNTP incorporation (direct labeling), 2) during a subsequent reverse transcription reaction via modified-dNTP incorporation (direct labeling), or 3) in a coupling reaction after aRNA synthesis where the label is added to an amino allyl-modified rNTP incorporated during IVT or reverse transcription (indirect labeling). Increasingly, amino allyl incorporation combined with a secondary dye coupling reaction is being used for the generation of samples for array
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Custom siRNA Oligo Synthesis Service
2. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
3. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
4. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
5. New Microarray Labeling Kit for Preparing cDNA Probes
6. Amino Allyl Labeling for Array Analysis
7. Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
8. Efficient Europium Labeling of Lymphokine-Activated Killer Cells Using the Gene Pulser II with RF Module, Rev C
9. USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5-Ends
10. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
11. Human Universal cDNA Library Array I

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: aRNA Synthesis and Labeling for Array Analysis

(Date:11/28/2009)...Vanuatu National Advisory Committee on Climate Cha...ion (SNC) Project in collaboration with the Vanuat...New Zealand have generated preliminary climate cha...background for the country’s SNC reporting t...te Change (UNFCCC). , Po...
(Date:11/27/2009)...chanised blue dye test alternative is helping br...ance one step closer for pharma-companies. It is a...ne developed by Sepha for use with blister packs...l and global pharma-companies such as Sanofi-Aveni...nd productivity improvements on the packaging line...
(Date:11/25/2009)... 25 Biotech firm Replikins Ltd., w...m the 1918 pandemic through the prediction, outbre...ay issued its latest biochemical analysis of the v... H1N1 ("Swine Flu") virus has dropped from its pea...break in the spring of 2009 to resting non-epidemi...
(Date:11/25/2009)...25 /PRNewswire/ - Cytochroma today announced the a...ectors. Dr. Moldt will replace Mr. Ulrik Spork as .... The number of Board members remains unchanged at...e Peter Moldt to the board and look forward to ben...roma continues to make progress in becoming a lead...
Breaking Biology Technology:Preliminary Climate and Sea Level Changes for Vanuatu Through the Application of SimCLIM 2Preliminary Climate and Sea Level Changes for Vanuatu Through the Application of SimCLIM 3Preliminary Climate and Sea Level Changes for Vanuatu Through the Application of SimCLIM 4Sepha's Blue Dye Test Alternative Brings ‘Complete Automation' To Pharma-Packaging Quality Assurance 2Sepha's Blue Dye Test Alternative Brings ‘Complete Automation' To Pharma-Packaging Quality Assurance 3Biological H1N1 Vaccines: Too Little, Too Late 2Biological H1N1 Vaccines: Too Little, Too Late 3Biological H1N1 Vaccines: Too Little, Too Late 4Biological H1N1 Vaccines: Too Little, Too Late 5Cytochroma Announces Appointment of Peter Moldt to Board of Directors 2
...w sensor structures that could be used in novel se...r in highly sensitive medical sensors, according t...etters . , The new ,nanosensors, which are based... and US research groups, could be tailor-made to i...es, for example poisons or explosives in transport...
...rstCall/ - EnWave Corporation (TSX-V: ENW) ("EnWav...l completion of tests using its recently developed...es and probiotics from the Danish group Danisco. T...greement signed with Danisco last year. The object...thod delivering high-speed continuous bulk powder ...
...elease is being issued by Council for American Med...m.com/cgi-bin/prnh/20090325/DC89081LOGO ) , , ...of medical science and discovery in the United Sta...to maintaining America,s leadership in medical inn...advocates, biotechnology industry representatives,...
Other Biology Technology:New security and medical sensor devices made possible by metallic nanostructures 2EnWave Announces Collaboration and Initial Testing Results with Danisco AS 2EnWave Announces Collaboration and Initial Testing Results with Danisco AS 3Council for American Medical Innovation Brings U.S. Leaders in Medicine, Biotechnology and Discovery Together in Chicago for 'Best and Brightest Forum on Medical Innovation' 2
(Date:11/24/2009)...protein that may help gut bacteria bind to the gas...probiotic producers to identify strains that are l...ics need to interact with cells lining the gut to ...rfaces in the gut they are more likely to stick ar... Nathalie Juge from the Institute of Food Research...
(Date:11/24/2009)...ber 24, 2009) -- The Wildlife Conservation Society... last remaining population of Siberian tigers has ...de of poaching and habitat loss. , W...als of what needs to be done to protect remaining ... The report was released by the Siberian Tig...
(Date:11/24/2009)...Spanish . , The presence of increased body f...bstances in the blood, hinders the loss and mainte...ct of the University of Navarra conducted by Estba...Pharmacy. , The project, entitled "A nutrigeneti...l of body weight and inflammation," examines how t...
Breaking Biology News(10 mins):A sticky solution for identifying effective probiotics 2Report shows dramatic decline in Siberian tigers 2It can be predicted the reaction obese patients will have to a diet 2Antipsychotic Drugs Spur Dramatic Weight Gain in Kids 60185 1Antipsychotic Drugs Spur Dramatic Weight Gain in Kids 60185 2Antipsychotic Drugs Spur Dramatic Weight Gain in Kids 60185 3Antipsychotic Drugs Spur Dramatic Weight Gain in Kids 60185 4More Swine Flu Vaccine Coming Soon 60183 1More Swine Flu Vaccine Coming Soon 60183 2More Swine Flu Vaccine Coming Soon 60183 3Phone Counseling for Depression Rings With Promise 60181 1Phone Counseling for Depression Rings With Promise 60181 2
... Radiological health expert Daniel Hayes, Ph.D., o...l Hygiene suggests that a form of vitamin D could ...ge from low levels of radiation. Writing in the I...ains that calcitriol, the active form of vitamin D...ld be used as a safe protective agent before or af...
..., The answer to successful revegetation of native ...necessarily relying on remnant local native vegeta...local native plants are the best source of seed fo... from CSIRO, Australia,s national science agency. ...ted to local conditions and therefore it would pro...
... AMES, Iowa -- Turtles nesting along the Mississip...g dates in response to rising temperatures, says a...nzen, a professor in ecology, evolution and organi...d also accumulated research going back decades in ...ut when they make nests and lay eggs. , "The resu...
Other Biology News:Could vitamin D save us from radiation? 2Turtles alter nesting dates due to temperature change says ISU researcher 2
Goat Anti-Human NP-1 (Alpha-Defensin-1) Polyclonal Antibody, Unconjugated from PeproTech
Rabbit Anti-Raf-1 (C-12)-G Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
Anti-Human Amyloid beta [1-16] Monoclonal Antibody, Unconjugated, Clone 6E10 from Cell Sciences
...Development kit contains the basic components requ...sure natural and recombinant mouse Dickkopf homolo...Each kit contains sufficient materials to run ELIS...ed that the following conditions are met: The ass...
Biology Products: