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Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

et al., 2000). The pSilencer expression plasmids included GFP for identification of transfected cells. Silencing of SytV and Syt IX levels did not change basal secretion. However, glucose-induced insulin release was decreased suggesting that inhibition may be by impairment of the exocytotic process and that Syt V and Syt IX act as positive modulators of exocytosis. The addition of the phorbol ester PMA, which potentiates insulin exocytosis distal to the increase of cytosolic Ca2+ showed that both proteins are directly involved in the Ca2+-dependent stimulation of exocytosis. The association of Syt IX with insulin granules in β-cells suggests that Syt IX may be the Ca2+ sensor for insulin secretion in β-cells.


References

Chapman ER (2002) Synaptotagmin a Ca 2+ sensor that triggers exocytosis? Nat Rev Mol Cell Biol 3:498-508.

Gut A, Kiraly CE, Fukuda M, Mikoshiba K, Wollheim CB, Lang J (2001) Expression and localisation of synaptotagmin isoforms in endocrine beta-cells: their function in insulin exocytosis. J Cell Sci 114:1709-1716.

Iezzi M, Regazzi R, Wollheim, CB (2000) The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis. FEBS Lett 26:66-70.

Nagamatsu S, Nakamichi Y, Yamamura C, Matsushima S, Watanabe T, Ozawa S, Furukawa H, Ishida H
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