Navigation Links
Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

ncreatic islets. Confocal microscopy showed that SytV and Syt IX co-localize with insulin-containing secretory granules which was confirmed by using a continuous sucrose density gradient. Interestingly, studies performed in primary pancreatic endocrine cells revealed that Syt V is present in glucagon-containing islet alpha-cells but is not expressed in β-cells whereas Syt IX is associated with insulin granules in β-cells.

To investigate the function of Syt V and Syt IX, the Syt isoforms were over-expressed or their levels reduced using RNA interference. Both proteins were produced at similar levels but the anti-Syt IX antibody recognised a smaller and a larger form of Syt IX. The two forms of Syt IX may be due to post-translational modification such as O-glycosylation and palmitoylation. Transient over-expression of Syt V and Syt IX did not alter exocytosis in INS-1E cells indicating that both proteins do not interfere with the stoichiometry of SNARE proteins whose over-expression has been shown to inhibit insulin exocytosis (Nagamatsu et al., 1999).

To investigate the hormone release, the pSilencer SytV and Syt IX siRNA expression plasmids were each transiently cotransfected into INS-1E cells with a plasmid encoding the human growth hormone hGH. hGH is targeted to insulin-containing granules and can be used to monitor exocytosis as an insulin substitute in a subpopulation of transfected cells (Iezzi
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Your Data: Detection of Oocyte-specific Gene Expression Using Ambions Cells-to-cDNA Kit
2. Your Data: RNAlater Stories
3. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
4. Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs
5. Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA
6. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
7. Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
8. Custom and library siRNA for efficient gene silencing
9. Custom and library siRNA for efficient gene silencing
10. Cancer siRNA Oligo Set Version 1.0
11. Library siRNA
Post Your Comments:
(Date:8/28/2015)... ... August 28, 2015 , ... Riordan-McKenna Institute ... DO, announced today that the use of sterile, dehydrated amniotic tissue AlphaPATCH™ developed ... an otherwise non-healing surgical knee wound. , The case involved a 78-year-old male, ...
(Date:8/27/2015)... ... 27, 2015 , ... The Kansas City Animal Health Investment ... health companies to hear from animal health companies with the newest technology. Companies ... agreements or distribution contracts. This meeting is one of the only investment ...
(Date:8/27/2015)... Mich. , Aug. 27, 2015  Neogen Corporation ... acquired the stock of United Kingdom ... of microbiological culture media and diagnostic systems. ... grown into a leading provider of microbial testing and ... clinical testing markets. The company currently sells into more ...
(Date:8/27/2015)... CELEBRATION, Fla. , Aug. 27, 2015 ... announced that a patient,s eyes—and life—were saved by doctors ... , working with NNFF, NovaBay Pharmaceuticals and Dr. ... Wound Care Center at Seton Medical Center in ... The successful treatment used a breakthrough approach, pioneered by ...
Breaking Biology Technology:Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 2VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 3Neogen acquires U.K.-based Lab M Holdings 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3
... N.J., Oct. 6 NEXCORE Technology, Inc., a,world-class ... an agreement with GI Supply of Camp Hill, ... Wand. Polar Wand is a portable,device that uses ... the gastrointestinal tract in patients resistant to conventional,endoscopic ...
... Environmental,Tectonics Corporation,s (Amex: ETC ) ("ETC" or ... of two (2) BARA-MED(R) XD,Monoplace Hyperbaric Chambers to ... delighted to work with Saint Barnabas Medical Center, ... one of the largest health care providers in ...
... BioMed Realty Trust, Inc.,(NYSE: BMR ) today ... its over-allotment option in full to purchase an ... BioMed,s previously,announced offering that priced on October 1, ... common stock, raising net proceeds of,approximately $212.4 million ...
Cached Biology Technology:NEXCORE Technology Selected to Manufacture New Endoscopic Cryotherapy Device 2Environmental Tectonics Corporation Announces the Placement of Two Monoplace Hyperbaric Chambers at Saint Barnabas Medical Center in New Jersey 2Environmental Tectonics Corporation Announces the Placement of Two Monoplace Hyperbaric Chambers at Saint Barnabas Medical Center in New Jersey 3Environmental Tectonics Corporation Announces the Placement of Two Monoplace Hyperbaric Chambers at Saint Barnabas Medical Center in New Jersey 4BioMed Realty Trust Announces Exercise of Over-Allotment Option 2BioMed Realty Trust Announces Exercise of Over-Allotment Option 3
(Date:7/31/2015)... de julho de 2015 A 10 a. ... será realizada pela BGI de 22-25 de outubro de 2015, ... A conferência está celebrando seu 10 o. aniversário ... se tornou uma das reuniões mais influentes do mundo ... mais dinâmicos, entusiastas e prazerosos. A ...
(Date:7/31/2015)... 2015 La 10 th International Conference on ... BGI del 22 al 25 de octubre de 2015 en ... la conferencia celebra su décimo aniversario. Desde su inauguración en ... las reuniones anuales más influyentes del mundo en el campo ... entusiastas y mejores a nivel científico. ICG-10 ...
(Date:7/31/2015)... --NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... mobile commerce market and creator of the Wocket® smart ... for ELECTRONIC CRYPTO-CURRENCY MANAGEMENT METHOD AND SYSTEM ... crypto-currencies such as Bitcoin into the consumer market of ... manage all payments.  The technology manages ...
Breaking Biology News(10 mins):A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3NXT-ID Patents Electronic Crypto-Currency Management Technology 2NXT-ID Patents Electronic Crypto-Currency Management Technology 3NXT-ID Patents Electronic Crypto-Currency Management Technology 4
... ear. Sylvia Plath stuck her head in the oven. History ... ways. Were these artists simply mad or brilliant? According to ... of the Association for Psychological Science, maybe both. In ... Szabolcs Kri of Semmelweis University in Hungary focused his research ...
... first experiences with exotic species in the Basque Country, ... concretely in Laukiz, Lezama and Alonsotegui (Muro, 1975) where ... expansion of rapid growth plantations such as those of ... of the growing paper pulp industry. Blights and diseases ...
... MADISON, WI, SEPTEMBER 28, 2009 -- Emphasizing cross-disciplinary ... mass-volume relationships, can help undergraduates learn real-world, problem-solving ... soil science careers. Drs. Josh Heitman and ... Department, highlighted this need for quantitative measurement skills ...
Cached Biology News:Mad genius: Study suggests link between psychosis and creativity 2Canker disease in eucalyptus in the Basque Country 2Canker disease in eucalyptus in the Basque Country 3Canker disease in eucalyptus in the Basque Country 4Help students think like soil scientists 2Help students think like soil scientists 3
... the first biological reagent that ... and not just by inhibiting ... anti-mycoplasma agent that can be ... directly. The cytotoxicity of Mynox ...
A component of the Acridinium protein labeling kit....
... can be used to label ... covalently bound acridinium NHS ester ... presence of hydrogen peroxide. Acridinium ... used as a detection method ...
... Guava is making flow cytometry accessible to anyone ... appreciate the latest addition to Guavas on-demand microcapillary ... easy to use, surprisingly affordable, very compact, ... 3-color detection. The Guava EasyCyte Mini has all ...
Biology Products: