Navigation Links
Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

ural stem cells, which grow as neurospheres in the culture medium, a 50% reduction in protein synthesis of the target gene was observed. The lower efficiency of gene silencing in these primary culture cells can be attributed to inefficient transfection observed with cells grown in suspension and poor transport of the siRNAs to the core of the sphere. Cells transfected with Oligofectamine alone or single-stranded RNA as controls did not show altered levels of the targeted protein. Moreover, nonspecific RNAi effects, such as activation of the PKR pathway, were not observed. In some cases, when growth factors were removed from the culture medium, differentiation of the primary neural stem cells, which normally give rise to neuronal and glial cells, was altered in the siRNA treated cells. siRNA-mediated gene silencing is thus proving to be a powerful tool for the study of differentiation of mouse neuronal primary cells.


References

1. Zamore PD, Tuschl T, Sharp Pa, Bartel DP. (2000) RNAi:
double stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell. 101(1): 2533.

2. Elbashir SM, Lendeckel W, and Tusch T (2001) RNA interference is mediated by 21- and 22- nucleotide RNAs. Genes Dev. 15(2): 188200.


back to top


Ordering Information
For prices and availability, please contact our Customer Service Department.
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Your Data: Detection of Oocyte-specific Gene Expression Using Ambions Cells-to-cDNA Kit
2. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
3. Your Data: RNAlater Stories
4. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
5. Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs
6. RNAi Gene Silencing System
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
9. Drive Long-Term Silencing with CMV Vectors
10. Enhanced siRNA Delivery and Long-term Gene Silencing
11. Fast, Accurate Assessment of siRNA-induced Gene Silencing
Post Your Comments:
(Date:11/26/2014)... Whitehouse Laboratories is pleased to announce ... (New Jersey) Chamber of Commerce as a member. ... in the North Jersey area, offers Whitehouse Laboratories ... clients in the pharmaceutical and biotechnology areas that ... enables Whitehouse Laboratories a higher level of visibility ...
(Date:11/26/2014)... November 25, 2014 Global biostimulants ... 2019 growing at an estimated CAGR of 12.5%. ... healthy food products with least ecological impacts, farmers ... fertilizers to boost crop yield more effectively & ... that can improve the return on investments. , ...
(Date:11/26/2014)... Theravalues Corporation est fier d,annoncer le lancement de ... Hi Europe 2014 (du 2 au 4 décembre à ... la plus biodisponible actuellement sur le marché, elle ... approuvés par les règlements européens. La ... racine de curcuma ( Curcuma longa ) qui ...
(Date:11/24/2014)... 2014 Five American winners will ... Maktoum Awards for Medical Sciences", in its 8 th ... Emirates , on 15 December 2014. ... The Carter Center which won the Hamdan Award for ... numerous programs that aim to improve health and promote ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 32019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 32019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 4La curcumine présentant la plus haute biodisponibilité bientôt en vente en Europe 2La curcumine présentant la plus haute biodisponibilité bientôt en vente en Europe 3Five US Winners Among Recipients of Hamdan Medical Awards 2
... Milestones, CRANBURY, N.J., Nov. 3 Amicus Therapeutics,(Nasdaq: ... third quarter of,2008. On a reported basis calculated in ... a net loss,attributable to common stockholders of $0.36 per ... three months ended September 30, 2008. As of,September 30, ...
... Nov. 3 Biomoda, Inc. (OTC,Bulletin Board: BMOD) ... announced today that BUYINS.NET, http://www.buyins.net, is initiating ... data to October 2008. From November 2006 to ... BMOD have traded,for a total dollar value of ...
... and GUELPH, Ontario, Nov. 3 LI-COR,Biosciences ... agreement to,distribute LI-COR biotechnology products in Canada ... local presence of Mandel Scientific skilled staff, ... enhance our ability to serve,our Canadian customers," ...
Cached Biology Technology:Amicus Therapeutics Announces Third Quarter 2008 Financial Results 2Amicus Therapeutics Announces Third Quarter 2008 Financial Results 3Amicus Therapeutics Announces Third Quarter 2008 Financial Results 4Amicus Therapeutics Announces Third Quarter 2008 Financial Results 5Amicus Therapeutics Announces Third Quarter 2008 Financial Results 6Amicus Therapeutics Announces Third Quarter 2008 Financial Results 7Amicus Therapeutics Announces Third Quarter 2008 Financial Results 8Amicus Therapeutics Announces Third Quarter 2008 Financial Results 9Amicus Therapeutics Announces Third Quarter 2008 Financial Results 10Amicus Therapeutics Announces Third Quarter 2008 Financial Results 11Biomoda, Inc. (BMOD) SqueezeTrigger Price is $0.27. Approximately 1 Million Shares Shorted Since November 2006 According to Buyins.net Research Report 2Biomoda, Inc. (BMOD) SqueezeTrigger Price is $0.27. Approximately 1 Million Shares Shorted Since November 2006 According to Buyins.net Research Report 3Biomoda, Inc. (BMOD) SqueezeTrigger Price is $0.27. Approximately 1 Million Shares Shorted Since November 2006 According to Buyins.net Research Report 4LI-COR Biosciences and Mandel Scientific Company Inc. Announce Distribution Agreement 2
(Date:11/4/2014)... University School of Medicine announced today that it ... funded by the Bill & Melinda Gates Foundation. ... Gynecology and Reproductive Sciences; and Vice Chair of ... of Medicine will pursue an innovative global health ... to Assess Fetal Neurodevelopment.", Grand Challenges Explorations (GCE) ...
(Date:11/4/2014)... England , November 4, 2014 ... and vertical market growth   Fuel3D , ... has closed a funding round totaling $6.4 million (£4 million). ... million) funding secured earlier this year and paves the way ... in 2015. The funding round was led by ...
(Date:11/3/2014)... -- Research and Markets has announced the addition ... and Companies" to their offering. This ... have already started to play an important role in the ... old fashioned bone marrow transplants. Role of cells in drug ... a part of medical practice. Stem cells are ...
Breaking Biology News(10 mins):Temple University School of Medicine receives Grand Challenges Explorations grant 2Fuel3D Secures $6.4 Million in Expansion Funding 2Fuel3D Secures $6.4 Million in Expansion Funding 3Global Cell Therapy - Technologies, Markets and Companies: 2014 Report 2Global Cell Therapy - Technologies, Markets and Companies: 2014 Report 3
... HOUSTON, May 8, 2012 With the heart having limited ... cause of morbidity and mortality in the developed world. Research ... lecture May 15 at the University of Houston (UH). ... of Texas Southwestern Medical Center, will present "Heart Making and ...
... University scientist and designer from Africa have together created ... with insecticides for warding off mosquitoes infected with malaria, ... the continent. Though insecticide-treated nets are commonly used ... prototype garment can be worn throughout the day to ...
... Association of Pharmaceutical Scientists (AAPS) WHAT: The ... scientists from industry, government, and academia for three ... biotechnology sector of the pharmaceutical sciences. The conference ... Training Course and two AAPS Workshops, transitions into ...
Cached Biology News:New heart repair strategies discussed at UH lecture May 15 2African scientist, designer partner to fashion anti-malaria garment that wards off bugs 2
Mouse polyclonal antibody to ALS2CR8 - amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8...
...
Mouse monoclonal [CAT-2] to Chloramphenicol Acetyltransferase...
Recommended fro Western blotting application...
Biology Products: