Navigation Links
Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

cleotide RNA fragments, termed short interfering RNAs (siRNAs), which bind and tag the complementary portion of the target mRNA for nuclease digestion (1,2).

siRNA mediated gene silencing studies were carried out to assess the mechanisms of mouse neural stem cell differentiation. Silencing of Gadd153, a pro-apoptotic transcription factor, was first assessed in a mouse neuroprecursor cell line, C17.2. Chemically synthesized, PAGE purified siRNAs (Ambion) were transfected into cells grown to 4070% confluency in complete medium without antibiotics. Individual siRNAs (2080 nM) were mixed with Oligofectamine and Opti-MEM (Invitrogen) and incubated at room temperature for 20 min. This mixture was added to a minimal volume of serum-free medium and transferred to C17.2 cells. The cells were incubated for 4 hr at 37C after which medium containing serum was added. Subsequent to the addition of serum, the cells were incubated for 15 days and harvested for further analysis. Western analyses revealed 7585% silencing of the Gadd153 gene (Figure 1).

Figure 1. Gene Silencing in a Neuroprecursor Cell Line Using siRNA. siRNAs were designed to target Gadd153, a pro-apoptotic transcription factor, and were transfected into C17.2 cells using Oligofectamine and Opti-MEM (Invitrogen). Silencing efficiency was analyzed by Western blot. Control cells were treated with Oligofectamine alone, and -actin was used as a loading control. Quantitative analysis showed a knockdown efficiency of approximately 7585%.


When siRNAs targeting other proteins (to be published) were also tested on embryonic mouse primary ne
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Your Data: Detection of Oocyte-specific Gene Expression Using Ambions Cells-to-cDNA Kit
2. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
3. Your Data: RNAlater Stories
4. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
5. Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs
6. RNAi Gene Silencing System
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
9. Drive Long-Term Silencing with CMV Vectors
10. Enhanced siRNA Delivery and Long-term Gene Silencing
11. Fast, Accurate Assessment of siRNA-induced Gene Silencing
Post Your Comments:
(Date:7/23/2014)... July 23, 2014 OncLive® is ... Center has joined its Strategic Alliance Partnership program. ... Partnership program, UNC Lineberger will have the opportunity ... awareness of its cutting-edge research initiatives, community-directed cancer ... Lineberger will work with OncLive to educate their ...
(Date:7/23/2014)... July 23, 2014 Adult stem ... therapies are biological, but not magical. However, ... to treat them as if they were magical. ... unsanctioned studies that constitute “stem cell tourism,” are ... approach to developing new regenerative medicine therapies. In ...
(Date:7/22/2014)... 2014 (PRWEB) July 23, 2014 ... University. , The goal of the Symposium ... full spectrum of scientists working in the biomaterials ... topics that represent the most promising directions for ...     Provide faculty and government scientists ...
(Date:7/22/2014)... Even within a phylum so full of mean little ... arthropods for its cruelty -- at least to crickets. Native ... is a most predatory sort of parasite. It swoops onto ... of larvae, and leaves its wicked brood to invade, kill ... of this would be possible without the fly,s ability to ...
Breaking Biology Technology:OncLive Expands Its Strategic Alliance Partnership Program With the Addition of UNC Lineberger Comprehensive Cancer Center 2OncLive Expands Its Strategic Alliance Partnership Program With the Addition of UNC Lineberger Comprehensive Cancer Center 3The Adult Stem Cell Technology Center, LLC Continues to Develop Technologies for Homologous Adult Stem Cell Therapies and Drug Development 2The Adult Stem Cell Technology Center, LLC Continues to Develop Technologies for Homologous Adult Stem Cell Therapies and Drug Development 3The Adult Stem Cell Technology Center, LLC Continues to Develop Technologies for Homologous Adult Stem Cell Therapies and Drug Development 4The Adult Stem Cell Technology Center, LLC Continues to Develop Technologies for Homologous Adult Stem Cell Therapies and Drug Development 5The 2014 NJ Symposium on Biomaterials Science Earns Society for Biomaterials Endorsement 2Fly-inspired sound detector 2Fly-inspired sound detector 3Fly-inspired sound detector 4
... , June 15 /PRNewswire-Asia/ -- AgFeed Industries, Inc. ... hog production and animal nutrient companies in China , ... the first phase of the Xinyu production pod starting in ... a division of Genus PLC (LSE: GNS). This stocking will ...
... Cellular ... of bringing more relevant and highly predictive toxicity testing to the drug discovery process. ... Madison, ... into a research collaboration with the goal of bringing more relevant and highly predictive ...
... ... the life sciences industry that incorporates distinctive features and functionality , ... (PRWEB) June 15, ... life sciences industry, today announced the release of Version 2.0 of its Interactive ...
Cached Biology Technology:AgFeed Industries, Inc. Announces Stocking Agreement for Xinyu Production Pod 2AgFeed Industries, Inc. Announces Stocking Agreement for Xinyu Production Pod 3AgFeed Industries, Inc. Announces Stocking Agreement for Xinyu Production Pod 4Promega and Cellular Dynamics Partner to Validate Stem Cell-Based In Vitro Assays for Predicting Drug-Induced Cardiotoxicity 2Promega and Cellular Dynamics Partner to Validate Stem Cell-Based In Vitro Assays for Predicting Drug-Induced Cardiotoxicity 3Viscira Announces Version 2.0 of its Online Interactive Slide Kit Builder Application 2Viscira Announces Version 2.0 of its Online Interactive Slide Kit Builder Application 3
(Date:7/23/2014)... Currently, no cure exists for Alzheimer,s disease, the ... Americans. But scientists are now reporting new progress ... cancer treatment, that shows promise as a potential ... ACS, Journal of Medicinal Chemistry . , ... that in a healthy brain, the protein known ...
(Date:7/23/2014)... preconditioning has been confirmed by many studies, but ... released in the Neural Regeneration Research ... and co-workers from Tongji Hospital Affiliated to Tongji ... China performed cerebral ischemic preconditioning in rats by ... mechanism underlying the neuroprotective effect of ischemic preconditioning. ...
(Date:7/22/2014)... The exhibit consists of both still images and ... of scientific research. Forty-four still images were chosen ... students, postdocs, staff, and alumni representing more than ... from more than 50 submissions. , Zach Donnell, ... of the 2014 organizers, noted that the exhibit ...
Breaking Biology News(10 mins):Art of Science 2014 2
... 16, 2010 Primary care physicians agree they have a ... the right weight management resources. Obese or heavier adults ... lose weight may lack information about effective weight loss methods ... research commissioned and released today by the Strategies to Overcome ...
... programs to safeguard them from getting out of control ... cancer. Until now, researchers assumed that these protective systems ... first time, using an animal model for lymphoma, cancer ... the Charit University Hospital Berlin in Germany have ...
... to passive smoking are more likely to have health problems ... levels of nucleated red blood cells in the umbilical cord ... and Public Health . Pediatrician Abd ElBaky of the ... Cairo University, Egypt, have found that obesity and passive smoking ...
Cached Biology News:STOP Obesity Alliance surveys show doctors, patients share role in weight loss, but ask, now what? 2STOP Obesity Alliance surveys show doctors, patients share role in weight loss, but ask, now what? 32 at 1 stroke -- how cells protect themselves from cancer 2Obesity and passive smoking reduce oxygen supply to unborn baby 2
... Signet offers the most comprehensive, ... requirements. We have incorporated unprecedented quality ... capabilities. This includes proven detection chemistries ... to the TechMate protocol. It also ...
... Each Signet Level 2 Detection System ... avidin-biotin chemical bonding principle and Signets ... provide sensitivity, specificity and reliablility. Signet ... in both manual and automated protocols. ...
... Signet Level 2 Detection System combines ... chemical bonding principle and Signets unique ... sensitivity, specificity and reliablility. Signet reagents ... both manual and automated protocols. ...
Each bottle contains: Bromophenol Blue 0.25% w/v , Xylene Cyanol FF 0.25%, Glycerol solution 30% w/v...
Biology Products: