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Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA


One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the Silencer siRNA Construction Kit. While this kit is very cost effective when RNAs of several different sequences need to be made, it can be advantageous to turn to chemical synthesis once a specific siRNA sequence is needed in bulk. Ambion also offers a Custom siRNA Oligonucleotide Synthesis Service. siRNA oligonucleotide pairs are provided after optimized deprotection and reverse phase desalting, and for a limited time, free PAGE purification (for more information see, "Cost Effective High Potency siRNAs"). Here Milhavet and Mattson, researchers at the NIH, use PAGE purified siRNAs from Ambion to study gene function during mouse neural stem cell differentiation.

Ollivier Milhavet and Mark P. Mattson
Laboratory of Neurosciences,
National Institute on Aging, Gerontology Research Center,
National Institutes of Health, Baltimore, MD 21224

RNA interference (RNAi) is the phenomenon by which double-stranded RNA (dsRNA) specifically suppresses the expression of a gene with homologous sequence at the post-transcriptional level. In C. elegans, D. melanogaster and mammalian cell cultures this effect has been shown to be mediated by 2123 nu
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