Navigation Links
Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs


Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the Silencer siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, researchers at the Salk Institute describe the successful silencing of two neural cell adhesion proteins using siRNAs produced by this kit.

Drs. Qi Chen and David Schubert at the Salk Institute recently used in vitro transcribed siRNAs, prepared with Ambion's Silencer siRNA Construction Kit, to study two genes that are involved in nerve cell adhesion. These researchers sought to determine whether siRNA-mediated silencing of Gene 1 would affect the protein expression levels of a putative downstream gene, Gene 2. To this end, the researchers prepared six siRNAs by in vitro transcription that targeted Gene 1. These siRNAs were transfected individually into the nerve cell line B103, and the protein expression levels of both genes were monitored by Western blot 96 hours post-transfection.

One of the six gene specific siRNAs effectively reduced Gene 1 protein levels (Figure 1A). Interestingly, the same siRNA also reduced the protein levels of Gene 2, but not those of the -actin control (Figure 1B and C). These results suggested that Gene 1 does indeed positive
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Your Data: Detection of Oocyte-specific Gene Expression Using Ambions Cells-to-cDNA Kit
2. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
3. Your Data: RNAlater Stories
4. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
5. Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA
6. RNAi Gene Silencing System
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
9. Drive Long-Term Silencing with CMV Vectors
10. Enhanced siRNA Delivery and Long-term Gene Silencing
11. Fast, Accurate Assessment of siRNA-induced Gene Silencing
Post Your Comments:
(Date:7/31/2014)... The ability to see through organs and even the ... well as fine-grained cellular structures has been a long-time ... Press July 31st in the journal Cell ... simple methods for making opaque organs, bodies, and human ... connections intact. The protocols could pave the way for ...
(Date:7/31/2014)... Colo. , July 31, 2014 ... awardee today with $25,000 to fund a translational ... inception in 2011, the gift was presented at ... Annual Meeting in Colorado Springs, ... specialize in neurointerventional approaches to neurovascular conditions, SNIS ...
(Date:7/31/2014)... 2014 Graphite Metallizing Corporation announces ... carts. These bearings are uniquely suited to prevent loss ... because of the high temperatures . , Recently, ... the design engineers at Graphite Metallizing to overcome problems ... their manufacturing operation, pans containing blocks of powder mixture ...
(Date:7/31/2014)... 2014 Research and Markets has ... 2014-2018" report to their offering. ... study of the genetic material or genomes of an ... for the discovery and development of diagnostic and therapeutic ... conditions. Genomics is supported by three major product segments: ...
Breaking Biology Technology:See-through organs and bodies will accelerate biomedical discoveries 2SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 2SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 3Graphite Metallizing Announces Bearings for High Temperature Oven Applications 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 3Global Genomics Market 2014-2018: Key Vendors are F. Hoffmann-La Roche, Life Technologies, Qiagen and Illumina 2
... BUDAPEST, Hungary, August 21 ChemAxon, a software,solutions ... Instant JChem, it,s desktop application for working with ... The version 2 release brings,forms and form building, ... Instant JChem Personal edition features the vast,majority of ...
... Companies, PITTSBURGH, Aug. 21 The Pittsburgh ... region,s life,sciences industry on a fast track for ... of $450,000 in three companies: Applied Computational,Technologies, Separation ... committed to identifying and making investments in promising,life ...
... Dr. Lanza for ,Courage Under Fire, WORCESTER, ... Bulletin Board: ACTC) is proud to announced that ... and Scientific,Development, has been recognized for his research ... the current issue of PharmaVOICE (cover,story July/August issue). ...
Cached Biology Technology:Instant JChem 2.0 Released: Instant JChem Personal Free for All Users 2The Pittsburgh Life Sciences Greenhouse Adds New Companies to its Investment Portfolio 2The Pittsburgh Life Sciences Greenhouse Adds New Companies to its Investment Portfolio 3Advanced Cell Technology's Dr. Robert Lanza Makes List of '100 Most Inspiring People in the Life-Sciences Industry' 2Advanced Cell Technology's Dr. Robert Lanza Makes List of '100 Most Inspiring People in the Life-Sciences Industry' 3
(Date:7/31/2014)... New research, supported by the National Science Foundation ... permafrost uniformly accelerates atmospheric warming, indicating instead that ... they emit into the atmosphere. , The ... Nature , focuses on thermokarst lakes, which occur ... fill with melted fresh water, converting what was ...
(Date:7/31/2014)... NCT00518336) shows the sustained efficacy, immunogenicity and ... vaccine Cervarix. Women vaccinated with the HPV-16/18 ... nine years, and vaccine efficacy (VE) against ... longest follow-up report for a licensed HPV ... full paper. , HPV and vaccination ...
(Date:7/31/2014)... An experimental vaccine protected 100 percent of animal models ... difficile , which causes an intestinal disease that kills ... of print in Infection and Immunity . ... and non-human primates against the purified toxins produced by ... orogastric spore infection, a laboratory model that mimics the ...
Breaking Biology News(10 mins):Certain Arctic lakes store more greenhouse gases than they release 2Sustained efficacy, immunogenicity, and safety for GlaxoSmithKline's HPV vaccine 2Sustained efficacy, immunogenicity, and safety for GlaxoSmithKline's HPV vaccine 3C. difficile vaccine proves safe, 100 percent effective in animal models 2
... in humanity,s greatest achievements but scientists have found that working ... chimpanzees. A series of trials by scientists found ... also understand the need to help a partner perform their ... were given tools to get grapes out of a box. ...
... known risk factor for back pain and disc disease. ... Annual Meeting of the American Academy of Orthopaedic Surgeons ... in 6,779 patients undergoing treatment for spinal disorders with ... each patient,s age, gender, weight, smoking history, assessment of ...
... Stingrays living in one of the world,s most famous ... the Cayman Islands have profoundly changed their ways, ... on marine wildlife, reports a new study published March ... Researchers from Nova Southeastern University,s Guy Harvey Research ...
Cached Biology News:Origins of human teamwork found in chimpanzees 2Origins of human teamwork found in chimpanzees 3Tourist-fed stingrays change their ways 2
KAI 1 (G-2)...
Anti-Mouse Valpha 2 TCR, Purified (Clone B20.1) (rat IgG2a)...
Anti-OSMR Monoclonal Antibody Research Focus: cytokine/growth factor Storage: 4C Shipping Temperature: 4C...
... are designed for use with the ProQuest ... MaV203 contains deletions in the endogenous GAL4 ... two-hybrid systems. The strain also has leu2 ... bait and prey vectors. MaV203 contains three ...
Biology Products: