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RNAlater (2005) Acta Astronautica (56):623-628.

Scientists at the University of Florida are working with NASA studying plant biology, as plants are central components of the advanced life-support system that will support long-term occupation of orbital facilities and extended spaceflight missions. Plants are also an excellent model system to study the effects of abiotic stress. Most plant research requires that some of the sample collection be conducted while in orbit, which provides material that is uncompromised by landing and post-flight handling delays.

In flight, sample harvest and proper storage of harvested samples are not trivial. Of significant concern, is the harvesting and stowing of samples obtained during spaceflight experiments to maximize high quality RNA. Snap freezing in liquid nitrogen and storage at 80C is very effective in maintaining protein and nucleic acid integrity. However, current orbital cryogenic freezer designs hold temperature for a maximum of 35 days, making them suitable only for short spaceflights.

Storage of samples in chemical fixatives like RNAlater provides several options without compromising RNA integrity. Figure 3 shows RNA isolated from Arabidopsis and wheat samples exposed to a variety of storage conditions. The data simulate those obtained from harvesting plants with RNAlater during both short and long duration missions. The storage condition of 20C mimicked the 25C compartment of the freezers used on board. The presence of equimolar amounts of cytoplasmic and plastid rRNA indicate that there was no degradation of the RNA sample.

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