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Your Data: RNAlater Stories

Jaggi*, Andrea Oberli*, Anna Baltzer*, Janine Antonov* and Hans Jorg Altermatt+

*Department of Clinical Research, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland, +Pathology Langgasse, 3012 Bern, Switzerland

E-mail: rolf.jaggi@dkf.unibe.ch

Most molecular analyses require surgical tissue samples to be snap frozen, usually in the operating room, and shipped on dry ice before further processing. Surgery departments and most pathology laboratories are generally not equipped to snap freeze and store samples at -80C (nor to perform the molecular analyses themselves). Further, pathologists often need the entire piece of resected tumor for optimal assessment and pathological diagnoses. Consequently, tumor material for RNA isolation and molecular analysis can only be obtained after inspection by the pathologist. Dr. Jaggi and colleagues have established a protocol that treats cryosections with RNAlater resulting in high quality RNA without interfering with standard operating and pathology procedures. Molecular analysis of RNA prepared by this procedure can be closely correlated with histological analyses based on adjacent tissue.


Protocol

Four 20 m thick sections are transferred while maintaining low temperatures into pre-cooled 2 ml Eppendorf tubes.

Sections are quick
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