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Preserving RNA in Cryosections and Taking RNAlater into Space
RNAlater is an aqueous, non-toxic tissue storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue samples. RNAlater eliminates the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNAlater for storage, without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation. RNAlater can be added to cell pellets or cells in suspension and the samples stored frozen or unfrozen.

Here we describe two unique applications of RNAlater. In the first example, Dr. Rolf Jaggi and colleagues studied gene expression in tumor and normal adjacent tissue following histological examination. They developed a technique for treating tissue cryosections with RNAlater so that the sections could be used both for pathological review and for qRT-PCR. In the second example, Dr. Anna-Lisa Paul, Dr. Robert Ferl, and colleagues demonstrated successful pairing of RNAlater with Kennedy Space Center fixation tubes, allowing safe and effective preservation of plant material on spaceflights for subsequent molecular analyses back on earth.


Preserving Intact RNA in Cryosections with RNAlater

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