Navigation Links
Yeast Protein Production System Features High Yields and One-Step,,,Purification

The ESP system provides high-yield protein production in a eukaryotic organism

Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * Alan Greener
Stratagene Cloning Systems, Inc.

Stratagene's EPS yeast protein expression and purification systemllll provides novel opportunities for rapid, inexpensive and high-yield production of proteins in a eukaryotic organism. This system retains many eukaryotic posttranslational modifications of proteins that can be critical for the biological activity of expressed proteins. By using the yeast Schizosaccharomyces pombe as the host and glutathione-S-transferase (GST) as the protein purification tag, the ESP system expresses a protein of interest in yeast as a fusion protein with GST. The fusion protein is then purified using glutathione-agarose beads, and the GST tag can be removed from the protein by proteolytic cleavage with either bovine enterokinase or thrombin.

As numerous genes that are involved in transcriptional regulation and developmental control have been cloned and identified, the demand for homogeneously purified proteins for structural and functional analyses has increased. Although this demand can be partially fulfilled by prokaryotic expression systems, the analysis of eukaryotic proteins, whose functions are determined or influenced by posttranslational modifications, would require expression of the protein in a eukaryotic organism. Stratagene's ESP yeast protein expression and purification system has been created to meet this demand.

The yeast S. pombe is a unicellular eukaryotic organism, with properties that closely resemble higher eukaryotic organisms regarding chromosome structure and function, cell cycle control and RNA splicing.1 Stratagene's ESP system contains a cloning vector with both yeast and Escherichia coli replication origins, competent cell
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. A New C-Terminal GST Vector for Protein Production in S. pombe
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
10. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:8/28/2015)... ... 28, 2015 , ... Riordan-McKenna Institute founders, stem ... that the use of sterile, dehydrated amniotic tissue AlphaPATCH™ developed by Amniotic ... surgical knee wound. , The case involved a 78-year-old male, who presented with ...
(Date:8/27/2015)... , Aug. 27, 2015 /PRNewswire/ - Portage Biotech ... PBT.U), and Biohaven Pharmaceutical Holding Company Limited (Biohaven), ... Phase I study of BHV-0223, a glutamate modulating ... regarding BHV-0223 and recently obtained clearance from the ... with human testing. Portage holds 54% equity interest ...
(Date:8/27/2015)... YORK , August 27, 2015 ... enhancing image processing functions in the human vision system, announced ... point guard Chris Paul to develop ... players who want to improve their real-life on-court performance. ... the court, whether it is shooting a ball or blocking ...
(Date:8/26/2015)... KS (PRWEB) , ... August 26, 2015 , ... ... Biologic Solutions, announces today that a second US laboratory is to open in ... accordance with the continued partnership and long-term growth of research and development through ...
Breaking Biology Technology:Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 3MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3
... HOPKINTON, Mass. , May 18 Caliper Life Sciences, Inc. ... drug discovery and life sciences research, today announced that it reached an ... ® evaporation product lines to Biotage AB for $16.5 million ... , ...
... Sigma-Aldrich Corporation (Nasdaq: SIAL ) will be presenting at the Baird,s ... PM CDT ( 2:15 PM EDT ) in Chicago, IL. ... http://investor.sigmaaldrich.com/ .  Users can click the "Webcast" icon to access this file. ... About Sigma-Aldrich: ...
... , May 17 Three years of teamwork between ... Gunma University Heavy Ion Medical Center used carbon ion radiotherapy to treat ... the use of Elekta,s treatment planning software, XiO® , for delivery ... , , ...
Cached Biology Technology:Caliper Enhances Strategic Focus and Increases Growth Capital with Sale of Product Lines to Biotage 2Caliper Enhances Strategic Focus and Increases Growth Capital with Sale of Product Lines to Biotage 3Caliper Enhances Strategic Focus and Increases Growth Capital with Sale of Product Lines to Biotage 4Sigma-Aldrich Corporation to Present at the Baird's 2010 Growth Stock Conference on Thursday, May 20, 2010 2Elekta / Mitsubishi Electric Collaboration Helps Japan's Gunma University Heavy Ion Medical Center Launch Advanced Carbon Ion Therapy for Treating Cancer 2
(Date:8/4/2015)... Aug. 04, 2015 ... has announced the addition of the ... and Service), Sub-Segment (Computer Forensics, Network Forensics, ... Types, Service, Vertical and Region - Global ... offering. By Component (Hardware, Software, ...
(Date:7/31/2015)... 2015 BGI llevará a cabo la 10ª Conferencia ... 25 de octubre de 2015, en Shenzhen, ... décimo aniversario este año. Desde su inauguración en 2006, ... anuales más influyentes del mundo en los campos de ... más dinámicas, entusiastas y amenas. ICG-10 se ...
(Date:7/31/2015)... Die 10. internationale Konferenz zu Genomik ... und findet vom 22. bis 25. Oktober 2015 ... Die Konferenz feiert in diesem ... ist die ICG weltweit zu einem der einflussreichsten ... eines der dynamischsten, enthusiastischsten und angenehmsten wissenschaftlichen Zusammentreffen. ...
Breaking Biology News(10 mins):Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 2Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 3
... Researchers have shown that a peptide (a chain of ... tumor tissue. The peptide has been shown to substantially increase ... mice, achieving the same therapeutic effect as a normal dose ... paper published today in the online edition of the journal ...
... Gwen Laurie Smits Professor of Cell Biology, California ... the Ubiquitin, System and the N-End Rule Pathway ... clear that control of cellular functions through ubiquitin,conjugation ... in significance,classical regulation through transcription and translation. I ...
... in the south west of the Iberian Peninsula usually exceed ... plants in spring and summer, according to data gathered between ... of them next to the Doana National Park. The ... European Directive on ozone are usually exceeded from April onwards, ...
Cached Biology News:Cancer drug effectiveness substantially advanced 2Ozone levels tend to be above recommended levels at this time of year in South West Spain 2