phenotypes of glioma cells. In an effort to define the
contribution of each of these kinases, Dr. Joseph Loftus
Validated siRNAs targeting PTK2 and
PTK2B to knock down the expression of each of these kinases.
As seen in Figure 2, the expression of PTK2B was
significantly reduced following transfection of cells with the PTK2B validated
siRNA, but PTK2B expression was unchanged relative to the control in cells
transfected with the PTK2 validated siRNA. Similarly, PTK2 expression
was significantly reduced following transfection of cells with the PTK2
validated siRNA but unchanged following transfection with the PTK2B validated
siRNA. Successful suppression of PTK2 and PTK2B gene expression will allow
future analysis of the role of these kinases in integrin signaling and
other cellular phenotypes.
Figure 2. Specific
Gene Silencing of the Related Focal Adhesion Kinases FAK and Pyk2 .
HeLa cells were transfected with PTK2,
PTK2B, or a control (GAPDH) validated siRNA at a final concentration of
25 nM. 48 hours after transfection, cells were harvested and protein expression
levels were analyzed by Western blot with antibodies specific for Focal
Adhesion Kinase, FAK (PTK2), or Proline-rich tyrosine
kinase 2, Pyk2 (PTK2B).
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