phenotypes of glioma cells. In an effort to define the
contribution of each of these kinases, Dr. Joseph Loftus
Validated siRNAs targeting PTK2 and
PTK2B to knock down the expression of each of these kinases.
As seen in Figure 2, the expression of PTK2B was
significantly reduced following transfection of cells with the PTK2B validated
siRNA, but PTK2B expression was unchanged relative to the control in cells
transfected with the PTK2 validated siRNA. Similarly, PTK2 expression
was significantly reduced following transfection of cells with the PTK2
validated siRNA but unchanged following transfection with the PTK2B validated
siRNA. Successful suppression of PTK2 and PTK2B gene expression will allow
future analysis of the role of these kinases in integrin signaling and
other cellular phenotypes.
Figure 2. Specific
Gene Silencing of the Related Focal Adhesion Kinases FAK and Pyk2 .
HeLa cells were transfected with PTK2,
PTK2B, or a control (GAPDH) validated siRNA at a final concentration of
25 nM. 48 hours after transfection, cells were harvested and protein expression
levels were analyzed by Western blot with antibodies specific for Focal
Adhesion Kinase, FAK (PTK2), or Proline-rich tyrosine
kinase 2, Pyk2 (PTK2B).
back to top
Page: All 1 2 3 4 5
Related biology technology :
1. YOUR DATA:
Direct Real-time RT-PCR on Sympathetic Neuron Lysates
Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
2. Measuring RNase Activity A Real-time Kinetic Analysis
3. Ready for Real-time in 5 Minutes
4. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
5. Brilliant Core Reagent Kits for QPCR and QRT-PCR
6. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
10. RT-PCR Primers for the Study of Apoptosis
11. cMaster RTplusPCR System for increased sensitivity and product length
(Date:1/14/2014)... Calif. , Jan. 14, 2014 As pet owners drew ... doubt many of them wrote: "Take better care of my furry ... even designer clothes and top-brand carrying cases to take the little ... parents might also feel compelled to buy some pricey toys at ...
(Date:1/14/2014)... Jan. 14, 2014 The largest international professional ... plants and therapeutic derivatives thereof has endorsed an ... researchers about the challenges of adulterated herb and ... The Society for Medicinal Plant ...
(Date:1/14/2014)... iLab Solutions, the leader in core facility management software, ... Strategy. In this role, Michelle will work with current and ... the development of iLab products. Her main goal will be ... the scientific community by offering the most advanced, user-friendly, and ...
(Date:1/14/2014)... 2014 RXi Pharmaceuticals Corporation (OTCQX: RXII), a biotechnology ... therapies addressing major unmet medical needs using RNA-targeted ... of Allowance from the United States Patent and ... (sd-rxRNA®), for the treatment of fibrosis. The patent ... Breaking Biology Technology:New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3
... MADISON, N.J., Nov. 20 At a meeting of the ... today, a dividend of fifty cents($0.50) per share on the ... 2009 to stockholders of record at the close of business,on ... Wyeth is one of the world,s largest research-driven pharmaceutical and,health ...
... SIAL ),announced today the global release of MISSION(R) ... QUALITY and iScale Oligos(TM) siRNA are,expected to allow researchers ... with the highest purity and quality siRNA required for ... be well suited to target,validation and pre-clinical siRNA testing. ...
... 20 Nephros, Inc. (Amex: NEP ), a medical device company developing ... an update on its operations and strategy. , , ... , Appointed James Scibetta ... Ernest Elgin III President and CEO , Submitted ...Cached Biology Technology:Sigma-Aldrich Introduces MISSION(R) In VIVO QUALITY and iScale Oligos(TM) siRNA, a Superior Quality siRNA Designed for RNAi Research in Animal Models 2Nephros Provides Corporate Update 2Nephros Provides Corporate Update 3Nephros Provides Corporate Update 4Nephros Provides Corporate Update 5Nephros Provides Corporate Update 6Nephros Provides Corporate Update 7
(Date:4/24/2014)... fungus could now be treated in three days, rather than ... , Cryptococcus a form of yeast - ... research. They are found in many parts of the ... affect people with weakened immune systems. This infection kills up ... has tested the effects of the most commonly used drug ...
(Date:4/24/2014)... be improved with new insights into the make-up of ... the Amazon Basin have revealed unprecedented detail of the ... by comparing satellite maps with hundreds of field plots. ... more accurately the amount of carbon each tree can ... schemes, in which trees are given a cash value ...
(Date:4/24/2014)... of clear liquid can bend light, acting as a ... developed a new process to create inexpensive high quality ... , Because they,re so inexpensive, the lenses can ... to detect diseases in the field, scientific research in ... in classrooms. , "What I,m really excited about ... Breaking Biology News(10 mins):Treatment for deadly yeast disease reduced to 3 days 2Amazon rainforest survey could improve carbon offset schemes 2Bake your own droplet lens 2Bake your own droplet lens 3
p230 trans Golgi Purified Anti-Human clone 15, Isotype Mouse IgG 1 , 50 µg Consult technical datasheet for details....
... Probes intensely fluorescent and highly photostable ... use in a variety of applications ... strong signal. Unfortunately, protein- and other ... may cause them to bind to ...
DNA Polymerase I Large (Klenow) Fragment is a DNA polymerase that lacks the 5'Cut Site3' exodeoxyribonuclease activity of intact DNA Polymerase I but it contains the 3'Cut Site5' exodeoxyribonuclease...
... Description: Proteinase K is a highly active and ... applications purified from the fungus T. album. It ... of N-substituted hydrophobic aliphatic and aromatic amino acids ... protease. Proteinase K is useful in purifying high ...Biology Products: