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YOUR DATA: ,,, Direct Real-time RT-PCR on Sympathetic Neuron Lysates,,, ,,, Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

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Ambion, in partnership with Cenix BioScience, has manufactured and validated siRNAs targeting a number of important human genes. These Silencer Validated siRNAs are individual siRNA duplexes that have been verified experimentally to reduce the mRNA levels of their target genes by at least 70%. The use of validated siRNAs saves researchers both time and money that can be better spent answering specific biological questions. Here is one example of how validated siRNAs were used in gene silencing studies.


Silencing of Focal Adhesion Kinases
Malignant gliomas such as glioblastomas are a leading cause of CNS tumor-related death. At the root of the problem is the highly invasive behavior of glioblastomas. Glioblastoma migration, like that of other cell types, is a dynamic process ultimately dependent upon the interplay between the integrin family of cell adhesion receptors and extracellular matrix environments. Integrin receptors are known to affect the dynamic balance of various non-receptor tyrosine kinases that function as effectors of integrin mediated signaling. Dr. Joseph Loftus of the Mayo Clinic, Scottsdale has been examining the role of two potential proximal integrin effectors--the closely related focal adhesion kinases FAK (PTK2) and Pyk2 (PTK2B) -- in the temporal development of the proliferative or migrational
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