Navigation Links
YOUR DATA: ,,, Direct Real-time RT-PCR on Sympathetic Neuron Lysates,,, ,,, Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Sympathetic neurons were dissociated from the superior cervical ganglia of neonatal rats using dispase and collagenase. The neurons were plated onto regular tissue culture plastic for 2 hrs to remove non-neuronal cells, then grown on poly-ornithine and laminin coated 96-well plates for 7-9 days at a density of 1000-2000 cells per well. Cells were removed from the wells before lysis; this gave better results than lysis of these cells directly within the plate wells. 1000-2000 cells were processed per 100 l Lysis Buffer using the Cells-to-cDNA II Kit. This number of cells resulting in optimal PCR signal was determined by processing dilutions of cells and assaying for a positive control sequence spiked equally into each sample. Real-time PCR was performed on 2 l of the Cells-to-cDNA II reverse transcription reactions to amplify both DBH and GAPDH as an internal control for sample normalization (Figure 1). No DNA contamination was observed in the minus-RT controls.

Figure 1. Cytokine Suppression of DBH mRNA Expression. Neurons were treated with 100 ng/ml CNTF, 100 ng/ml CNTF + 20 M PD98059 (CNTF + PD), or 1% DMSO for 8 days. RNA extraction and reverse transcription were performed with the Cells-to-cDNA II Kit (A). DBH and GAPDH mRNAs were quantified using real-time PCR. DBH values were normalized to GAPDH, and expressed as percent of control (B). These data are the mean SEM from a single experiment. Each sample was assayed in triplicate.


Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
[
'"/>


Source:


Page: All 1 2 3 4 5

Related biology technology :

1. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
2. Measuring RNase Activity A Real-time Kinetic Analysis
3. Ready for Real-time in 5 Minutes
4. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
5. Brilliant Core Reagent Kits for QPCR and QRT-PCR
6. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
10. RT-PCR Primers for the Study of Apoptosis
11. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
Post Your Comments:
(Date:10/30/2014)... James Sherley says he has been working towards ... since his days as a principal investigator at the Fox ... Sherley founded the ASCTC as a new Boston biotech ... property developed in Sherley’s research over the years since FCCC, ... (MIT), and more recently as a senior scientist at the ...
(Date:10/30/2014)... 30, 2014 Isis Pharmaceuticals, Inc. (NASDAQ: ... management will present a company overview at the Nomura Biotechnology ... in Boston, MA. A ... "Investors & Media" section of the Company,s website, www.isispharm.com ... within 48 hours and will be archived for a limited ...
(Date:10/27/2014)... 27, 2014 Ryan Carfley , Managing ... to sit down with Bryan Hamilton, the publisher of the ... discuss talent in the Research Triangle region of North Carolina. ... highlighted at the roundtable include the role of social ... of work/life balance and the unique role that Millennials play ...
(Date:10/27/2014)... The “Molecular Diagnostic Market by ... INAAT, DNA Sequencing), End User (Hospital, Laboratories), & ... Forecast to 2018” provides a detailed overview of ... trends, and strategies impacting the global molecular diagnostics ... the revenue and share analysis. , The global ...
Breaking Biology Technology:Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 2Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 3Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 4Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2Personify's Ryan Carfley Talks Recruiting with Triangle Business Journal, RPG Solutions 2Personify's Ryan Carfley Talks Recruiting with Triangle Business Journal, RPG Solutions 3Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 2Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 3Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 4Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 5
... Bill Gates , Co-Chair and Trustee of the Bill & Melinda ... mobile technologies to improve health outcomes in the developing world at ... The summit, organized by the Foundation for the National Institutes of ... the mHealth Alliance, will be held November 8-10 in ...
... ROCKVILLE, Md. , Aug. 2 Vanda Pharmaceuticals Inc. (Vanda) (Nasdaq: ... and commercialization of products for central nervous system disorders, today announced it will ... 2010 , before the market opens. A full text copy of the release ... , , ...
... 2 Tengion Inc. (Nasdaq: TNGN ) ... the position of Chief Financial Officer.  Mr. Davis comes to Tengion after ... to develop improved protein therapeutics. At its peak, Neose employed 200 individuals, ... , , ...
Cached Biology Technology:Bill Gates Named Keynote Speaker at the 2010 mHealth Summit 2Bill Gates Named Keynote Speaker at the 2010 mHealth Summit 3Bill Gates Named Keynote Speaker at the 2010 mHealth Summit 4Vanda Pharmaceuticals to Announce Second Quarter 2010 Financial Results on August 5, 2010 2Tengion Appoints A. Brian Davis Chief Financial Officer 2
(Date:10/29/2014)... who lose their hair as a consequence of chemotherapy ... improve the scalp cooling technology that prevents hair loss. ... being pioneered by global scalp cooling manufacturing company, Paxman ... biology department of the University of Huddersfield. , The ... who has a background in the pharmacology of cancer ...
(Date:10/29/2014)... their food, but they also need protection from its harmful ... of molecules in plants that shields them from sun damage. ... American Chemical Society , one team reports on the mechanics ... and colleagues at Purdue University note that the harsh ultraviolet ... to plant DNA and, as a result, hinder plant growth. ...
(Date:10/29/2014)... German . ... Peter Schlögelhofer at the Max F. Perutz Laboratories (MFPL) ... of Vienna dived into the process of meiosis in ... an inversion of the standard meiotic phases. The researchers ... Nature Communications . , Meiosis is the two-step series ...
Breaking Biology News(10 mins):New technology on the way to aid cancer suffers who lose their hair after chemotherapy 2Meiotic cell division 'the other way round' 2
... Aluminum salts, or alum, have been injected into billions of ... one knows, however, how they boost the immune response. In ... the National Academy of Sciences researchers at National Jewish ... that host DNA coats the alum adjuvant and induces two ...
... N.C. -- The cost of complying with tougher EPA air-quality ... toward natural gas for electricity generation, according to a new ... particulate matter, nitrogen oxide and mercury may make nearly two-thirds ... as plants powered by natural gas, the study finds. ...
... Engineering Professor Tim Bender and Post-Doctoral Fellow Benoit ... polymer synthesis could have implications for the manufacture ... and even medical implants, the researchers say. ... subphthalocyanine polymers by facile coupling to poly (acrylic ...
Cached Biology News:Vaccine adjuvant uses host DNA to boost pathogen recognition 2Vaccine adjuvant uses host DNA to boost pathogen recognition 3New emissions standards would fuel shift from coal to natural gas 2Accidental discovery may lead to improved polymers 2
... agarose is used for second-dimension SDS-PAGE ... in place in PROTEAN Plus cells. ... to allow monitoring of electrophoresis runs. ... agarose in 1x Tris, glycine, SDS, ...
... HV high-voltage power supply, with temperature probe, 100-120 ... 5,000 V, 500 mA, and 400 W. The ... between 0 and 90 degrees C during electrophoresis. ... Power cord and instructions are provided. Dimensions are ...
The stirbar is used in the buffer tank of the Trans-Blot Plus cell to maintain uniform conductivity and temperature during electrophoretic transfer. Dimensions are 3.5 x 0.5 in....
... cooling coil, Trans-Blot cell, provides ... high-intensity, and extended electrophoretic transfer ... Trans-Blot cell. Use of the ... to an external water recirculator, ...
Biology Products: