( 15nm and 440nm. Finally the ratio of t...)XANTHOCHROMIA IN CEREBROSPINAL FLUID

Although the Chalmers and Kiley method shows good correlation with angiography tests for the detection of subarachnoid haemorrhage, the process of drawing and measuring by hand the tangent baseline and peak heights introduces too large a subjective error.

Thanks to the advanced Band Area and Height Correction feature, Cecil Instruments spectrophotometers provide a fast, automated procedure to carry out this analysis with unparalleled ease, accuracy and reproducibility.

The method is demonstrated here using a holmium oxide filter as an example. In a real CSF test the same steps shall be followed, and only the wavelengths will be changed to conform to the ones specified above.

Procedure:
Press the SCAN key to access the Scan menu

Select b Store Baseline if a baseline has not been recorded, or a WL Scan Menu otherwise. The following prompts will be shown:

Note: Values in bold were entered by the operator during the example run. Values in brackets are adequate for real CSF analysis.

This produces the following holmium oxide spectrum:

Press the QUANT key to access the following menu:

Select a Quantitative Methods. The next menu will be displayed:

Finally select f Band Area Reprocess. The instrument prompts for the following parameters:

Note: Values in bold were entered by the operator during the example run. Values in brackets are adequate for re
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