Navigation Links
Working With Laser Capture Microdissection Samples


Laser Capture Microdissection (LCM) is a method for obtaining pure populations of cells from heterogeneous samples. LCM permits the selection and capture of cells, cell aggregates, and discrete morphological structures from thin tissue sections. For LCM, frozen and OCT embedded, or formalin-fixed and paraffin embedded tissue sections are processed, stained, and dehydrated, then scanned under an inverted microscope. Cells are visualized through a thermoplastic film which is attached to the bottom of an optically clear microfuge tube cap. A laser pulse, directed onto the target cells through the film, melts the film and allows it to flow onto the targeted area where it cools and bonds with the underlying cell(s). The film including the adhered cells or clusters is then lifted. Captured cells can then be used for nucleic acid studies, including SNP analysis, endpoint and real-time RT-PCR, and mRNA expression profiling.


Formalin vs. Frozen Sections
LCM is usually carried out on frozen sections when samples will be used for RNA isolation. This is because formalin fixation introduces crosslinks between nucleic acids and proteins, resulting in fragmentation of the RNA. Freezing avoids crosslinking but introduces a different problem. Endogenous nucleases are not irreversibly inactivated, and can become active during the tissue processing steps (fixation, staining, destaining, and dehydration) carried out after sectioning.


An Improved Protocol for Staining
Ambion scientists performed a detailed analysi
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Maintaining High Sensitivity when Working with Small-Volume Samples
2. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
3. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
4. Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
5. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
6. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
7. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
8. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
9. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
10. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
11. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Working With Laser Capture Microdissection Samples

(Date:7/28/2014)... --  Propeller Health , the FDA-cleared digital health solution ... Brad Towle as Senior Vice President, Business and ... company,s sales growth, customer acquisition and program expansion. ... past role in the development of accountable care organizations, ... time in the industry," said David Van Sickle ...
(Date:7/28/2014)... California (PRWEB) July 28, 2014 The ... 604.40 billion by 2020, according to a new study ... with the rising demand for effective vaccines and drugs ... with unmet medical needs is expected to drive market ... need to enhance agricultural productivity via the use of ...
(Date:7/28/2014)... July 28, 2014 Research and Markets ... Analysis Market 2014-2018" report to their offering. ... analysis is a technique used to determine the size of ... It is crucial to analyze the size of particles as ... size analysis is performed to determine the behavior and nature ...
(Date:7/25/2014)... 2014 "Ischemic Stroke and Cerebral ... data on the Ischemic Stroke and Cerebral Stroke ... and data relating to the clinical trials on ... global Ischemic Stroke Research Report 2014 at: ... the trial numbers and their recruitment status as ...
Breaking Biology Technology:Propeller Health Hires Brad Towle as Senior Vice President of Business and Client Development 2Global Biotechnology Market Worth $604.40 Billion by 2020: Grand View Research, Inc 2Global Biotechnology Market Worth $604.40 Billion by 2020: Grand View Research, Inc 3Global Biotechnology Market Worth $604.40 Billion by 2020: Grand View Research, Inc 4Global Particle Size Analysis Market 2014-2018 2Ischemic Stroke and Cerebral Stroke Global Clinical Trials Review H2, 2014: MarketResearchReports.Biz 2Ischemic Stroke and Cerebral Stroke Global Clinical Trials Review H2, 2014: MarketResearchReports.Biz 3
... to be Held Wednesday, May 7, 2008 at 10:00 ... Emisphere,Technologies, Inc. (Nasdaq: EMIS ) will host a ... ended March 31, 2008 on,Wednesday, May 7, 2008 at ... conference call can be accessed through the,company,s website at: ...
... scientists first learned to make nanowires, the nano-sized wires ... taken many forms, including nanobelts, nanocoils and nanoflowers. , ... and graduate student Matthew Bierman accidentally made some pine ... and branches that tapered in length as they spiraled ...
... HER3 Assays to Provide Unique Insights and ... Cancer Therapies, SOUTH SAN FRANCISCO, May 1 ... the company,s HER1,and HER3 Quantitative Protein Assays are now ... the HER1 and,HER3 assays provide unique, quantitative measurements of ...
Cached Biology Technology:Emisphere Technologies, Inc. Announces Conference Call for First Quarter 2008 Financial Results 2Spiraling nanotrees offer new twist on growth of nanowires 2Spiraling nanotrees offer new twist on growth of nanowires 3HER1 and HER3 VeraTag(TM) Assays are Now Available for Use in Development and Clinical Evaluation of Cancer Therapeutics 2HER1 and HER3 VeraTag(TM) Assays are Now Available for Use in Development and Clinical Evaluation of Cancer Therapeutics 3HER1 and HER3 VeraTag(TM) Assays are Now Available for Use in Development and Clinical Evaluation of Cancer Therapeutics 4HER1 and HER3 VeraTag(TM) Assays are Now Available for Use in Development and Clinical Evaluation of Cancer Therapeutics 5
(Date:7/28/2014)... the Earth that are high priorities for conservation in ... vulnerable, as it has the lowest fraction of its ... in ,refugia, areas of biological diversity that support ... during times of great environmental change. The refugia that ... Scotland. , The biggest refugia are in the ...
(Date:7/28/2014)... CITY (July 28, 2014)The Brain & Behavior Research ... Klerman and Freedman Prizes, recognizing exceptional clinical and ... supported by NARSAD Young Investigator Grants. The grants ... in neurobiological and psychosocial research for the prevention, ... that affect one in four people. , Six ...
(Date:7/28/2014)... suffer from fertility problems, many of these due to ... might expect such genes, which reduce an individual,s ability ... the Weizmann Institute of Science that recently appeared in ... riddle. Not only can it explain the high rates ... in understanding the causes of genetic diseases and their ...
Breaking Biology News(10 mins):Study finds Europe's habitat and wildlife is vulnerable to climate change 2The Brain & Behavior Research Foundation names winners of Klerman and Freedman Prizes 2The Brain & Behavior Research Foundation names winners of Klerman and Freedman Prizes 3The Brain & Behavior Research Foundation names winners of Klerman and Freedman Prizes 4Mutations from Venus, mutations from Mars 2
... S-equol, a novel soy germ-based compound, is very likely ... dietary supplement SE5-OH, which is under development for reduction ... studies using an animal model presented at the Experimental ... advanced studies in menopausal women, and peer-reviewed reports have ...
... MIAMI - As Florida Governor Charlie Crist declared a ... scientists at the Center for Southeastern Tropical Advanced Remote ... actively collaborate with several international satellite data providers to ... The Deepwater Horizon oil platform caught fire after an ...
... first personalized treatment for lung cancer heralds the arrival ... will demand significant changes to the way cancer specialists ... today at the 2nd European Lung Cancer Conference in ... University of Vienna said that personalized therapy, in which ...
Cached Biology News:Natural S-Equol suggested as critical component in SE5-OH for reducing menopausal hot flushes 2Natural S-Equol suggested as critical component in SE5-OH for reducing menopausal hot flushes 3Natural S-Equol suggested as critical component in SE5-OH for reducing menopausal hot flushes 4Natural S-Equol suggested as critical component in SE5-OH for reducing menopausal hot flushes 5Making personalized lung cancer therapy a reality in Europe 2
...
D.R. Harper and C. Grose (1994) • Includes: virus structure & replication, fundamental virology, immunology and pathogenesis, vaccines and antiviral drugs, novel diagnostic techniques, cloning a...
... Desselberger (1995) • This book is ... classical and molecular techniques currently used ... and analysis of virus-specific antibodies and ... presented covering immunoassays, blotting and hybridization ...
...
Biology Products: