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Wideband sensitivity of the FluoroLog-3

fied in a Millipore Simplicity 185. Excitation of the sample occurred from 250 600 nm, in 50-nm increments. Each emission scan was recorded with a step size of 1 nm. The scans were taken under ambient room conditions.



Results and Discussion
The largest water Raman emissions are the 335-nm, 397-nm, and 463-nm peaks (see Figure 1), which also have the best signal-to-noise ratio, S/N (see Table 2). As the incident wavelength increases, the Raman peaks are displaced farther from the incident wavelength, but the energy loss is a constant 3380 cm1. Raman peaks are also visible at 275 nm, 531 nm, and 693 nm. The emission spectra are re-scaled to reveal the Raman bands more clearly at 667 nm and 753 nm (see Figure 2). These results demonstrate the high sensitivity of the SPEXFLUOROLOG-3 even at these long wavelengths.


Conclusions
Water-Raman transitions are detectable across a large spectral range. Therefore, the SPEXFLUOROLOG-3 demonstrates high sensitivity to fluorescence signals throughout the UV-visible region; see if your system can handle this task. Kinematically mounted gratings allow optimization of the spectrofluorometer for custom applications at various wavelengths. Gratings, blazed at different wavelengths and optimized for different spectral regions, are changed easily with instant alignment. The SPEX FLUOROLOG-3 reaches the statistical noise limit: this means that SPEX gives you the best system possible.


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