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Which Water to Use?

d for nuclease activity by incubating a sample overnight with a radiolabeled RNA or DNA probe. After incubation, the probe is run on a gel to detect any degradation from nuclease.

1. DEPC-treated Water - DEPC destroys enzymatic activity by modifying -NH2, -SH and -OH groups in RNases and other proteins. DEPC treatment is a very effective way to treat solutions that will contact RNA. Ambion's DEPC-treated water is autoclaved both before and after packaging to ensure sterility and complete inactivation of DEPC.

2. Nuclease-free Water (not DEPC-treated) - Ambion also offers nuclease-free water that is not DEPC-treated. Ambion's Nuclease-free Water is ideal for applications that are reported to be acutely sensitive to residual DEPC e.g., oocyte injection and other in vivo translation applications.

3. RT-PCR Grade Water - Ambion's RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using a sensitive end-point PCR assay based on detection of rRNA genes. Prokaryotic DNA contamination is assessed by performing PCR with 16S rRNA primers, and eukaryotic DNA contamination is assessed by performing PCR with 18S rRNA primers. Each lot is also tested for nuclease contamination. This product is ideal for use in any PCR or RT-PCR application.


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