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Which Water to Use?

by Sapna Chacko


High quality, DEPC treated, and non-DEPC treated water

Certified free of endonuclease, exonuclease, and RNase activity

Available in sizes to suit all your experimental needs

RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and sometimes even experimental failure. In trying to pinpoint the source(s) of RNase contamination, it is easy to overlook the water used in the experiment, either to prepare reagents, or to resuspend precipitated RNA. Even purified water can have a high pH and minerals that can interfere with reactions that require specific salt and pH conditions. Ambion offers three different grades of high quality water: DEPC-treated water, Nuclease-free water (not DEPC-treated) and RT-PCR grade water.


Ambion Certified and Guaranteed Nuclease-free
All Ambion water products are Type II 18 ohm filtered, and are certified to be nuclease-free. Our water undergoes state-of-the-art reverse osmosis filtration and deionization, followed by autoclaving and sterile filtration. Ambion's water purification equipment is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications. Finally, in order to be certified nuclease-free, each lot of water is teste
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