Navigation Links
Which Water to Use?

by Sapna Chacko


High quality, DEPC treated, and non-DEPC treated water

Certified free of endonuclease, exonuclease, and RNase activity

Available in sizes to suit all your experimental needs

RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and sometimes even experimental failure. In trying to pinpoint the source(s) of RNase contamination, it is easy to overlook the water used in the experiment, either to prepare reagents, or to resuspend precipitated RNA. Even purified water can have a high pH and minerals that can interfere with reactions that require specific salt and pH conditions. Ambion offers three different grades of high quality water: DEPC-treated water, Nuclease-free water (not DEPC-treated) and RT-PCR grade water.


Ambion Certified and Guaranteed Nuclease-free
All Ambion water products are Type II 18 ohm filtered, and are certified to be nuclease-free. Our water undergoes state-of-the-art reverse osmosis filtration and deionization, followed by autoclaving and sterile filtration. Ambion's water purification equipment is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications. Finally, in order to be certified nuclease-free, each lot of water is tested for nuclease activity by incubating a sample overnight with a radiolabeled RNA or DNA probe. After incubation, the probe is run on a gel to detect any degradation from nuclease.

1. DEPC-treated Water - DEPC destroys enzymatic activity by modifying -NH2, -SH and -OH groups in RNases and other proteins. DEPC treatment is a very effective way to treat solutions that will contact RNA. Ambion's DEPC-treated water is autoclaved both before and after packaging to ensure sterility and complete inactivation of DEPC.

2. Nuclease-free Water (not DEPC-treated) - Ambion also offers nuclease-free water that is not DEPC-treated. Ambion's Nuclease-free Water is ideal for applications that are reported to be acutely sensitive to residual DEPC e.g., oocyte injection and other in vivo translation applications.

3. RT-PCR Grade Water - Ambion's RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using a sensitive end-point PCR assay based on detection of rRNA genes. Prokaryotic DNA contamination is assessed by performing PCR with 16S rRNA primers, and eukaryotic DNA contamination is assessed by performing PCR with 18S rRNA primers. Each lot is also tested for nuclease contamination. This product is ideal for use in any PCR or RT-PCR application.


Bulk and Custom Orders
All of Ambion's water products can be packaged into larger custom sizes to fit your needs. Contact us at custom@ambion.com for more information.

Inactivate RNases in Solution

DEPC treatment of solutions that come in contact with RNA can be time-consuming, possibly hazardous, and only eliminates RNases present at the time of treatment. Some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all.

The RNAsecure Reagent eliminates these problems. RNAsecure is a unique nonenzymatic reagent that will irreversibly inactivate RNases in solution. Unlike DEPC, RNAsecure can be used with virtually any solution and does not require post-treatment autoclaving. The inactivation process can be repeated at any time to protect against introduced contaminants. RNAsecure does not interfere with downstream procedures, such as RT-PCR and in vitro transcription, and is also compatible with Tris and other solutions that cannot be treated with DEPC.

The RNAsecure Resuspension Solution contains the same active ingredients as the RNAsecure Reagent, but is supplied at working concentration for direct resuspension of RNA pellets.


back to top


Ordering Information
Cat# Product Name Size 1964 RNaseAlert Lab Test Kit 25 rxns 1966 RNaseAlert QC System 5 x 96 assays 1970 DNaseAlert QC System 5 x 96 assays 7005 RNAsecure Reagent 1 ml 7006 RNAsecure Reagent 10 ml 7010 RNAsecure Resuspension Solution 10 x 1 ml 9916 DEPC-treated Water 5 x 100 ml 9920 DEPC-treated Water 500 ml 9922 DEPC-treated Water 1 L 9924 DEPC-treated Water 4 x 1 L 9930 Nuclease-free Water (not DEPC-treated) 500 ml 9932 Nuclease-free Water (not DEPC-treated) 1 L 9934 Nuclease-free Water (not DEPC-treated) 4 x 1 L 9935 RT-PCR Grade Water 10 x 1.75 ml 9937 Nuclease-free Water (not DEPC-treated) 10 x 50 ml 9938 Nuclease-free Water (not DEPC-treated) 100 ml 9939 Nuclease-free Water (not DEPC-treated) 5 x 100 ml
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. PCR Grade Water
2. Enjoy a Greater Sense of Security with PCR-Grade Water
3. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Which Water Use

(Date:12/17/2014)... Bellingham, Washington, USA (PRWEB) December 16, 2014 ... Kirstin Baum, manager at Philipps- Universität Marburg’s Department ... optics and photonics, in the newly released ... 2015-2016. “And never be too shy to ask ... STEM (science, technology, engineering, and mathematics) occupations ranging ...
(Date:12/17/2014)... IN (PRWEB) December 17, 2014 ... Laboratory Excellence following its most recent COLA inspection. ... by COLA, a national healthcare accreditation organization. Accreditation ... standards of quality in day-to-day operations, demonstrate continued ... pass a rigorous on-site laboratory survey. ...
(Date:12/17/2014)... CASI Pharmaceuticals, Inc. (Nasdaq: CASI ... and commercialization of innovative therapeutics addressing cancer and other ... primary commercial focus on China , ... C meeting with the U.S. Food and Drug Administration ... February 2015.  During this meeting the Company will seek ...
(Date:12/15/2014)... 2014 Organovo Holdings, Inc. (NYSE MKT: ... delivering breakthrough 3D bioprinting technology,  today celebrated continued ... its exVive3D TM Human Liver Tissue.  The ... commercial release on November 18, has been recognized ... awards for innovation. Most recently, on ...
Breaking Biology Technology:Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 3Xeno Diagnostics Receives 3rd Award for Laboratory Excellence 2CASI Pharmaceuticals Granted Type C Meeting With U.S. FDA For ENMD-2076 In Fibrolamellar Carcinoma 2CASI Pharmaceuticals Granted Type C Meeting With U.S. FDA For ENMD-2076 In Fibrolamellar Carcinoma 3CASI Pharmaceuticals Granted Type C Meeting With U.S. FDA For ENMD-2076 In Fibrolamellar Carcinoma 4CASI Pharmaceuticals Granted Type C Meeting With U.S. FDA For ENMD-2076 In Fibrolamellar Carcinoma 5Organovo Celebrates a Positive 2014 Reception of its exVive3DTM Human Liver Tissue Product 2Organovo Celebrates a Positive 2014 Reception of its exVive3DTM Human Liver Tissue Product 3
... company developing what it believes to be a novel tool ... tax credits from the State of Wisconsin to ... instead has raised a total of $385,000 from a combination ... to CEO Larry Wells, state law prohibits the company from ...
... Wis. - Last week, the nation marked ... stem cell research. Those restrictions have unquestionably set back ... say. , ,When Congress passed legislation that would ... among families whose loved ones are suffering from a ...
... - After months of speculation, North Prairie Productions, ... selected a site for a biodiesel plant that will produce ... will begin construction on the $42 million facility next spring ... side, where it will relocate its Waterloo offices. , ...
Cached Biology Technology:NovaScan decides against tax credits 2Stem cell research holds the promise for tomorrow 2Stem cell research holds the promise for tomorrow 3Evansville site chosen for biodiesel plant 2
(Date:12/17/2014)... 2014  HITLAB SM announced today its ... to confirm its adherence to current U.S. Food ... HITLAB to conduct regulated smart device and smart ... patient safety and research quality. "HITLAB ... and delivery with innovative technology," said Laura ...
(Date:12/17/2014)... Research and Markets ( http://www.researchandmarkets.com/research/9zq33j/global_chemical ... "Global Chemical Sensor Market 2015-2019" report to ... One major trend upcoming in ... sensors in biomedical applications. Chemical sensors help in ... diagnosis during surgical procedures. The Global Chemical Sensor ...
(Date:12/15/2014)... Research and Markets ( http://www.researchandmarkets.com/research/87pph8/global_facial ... "Global Facial Recognition Market 2015-2019" report to ... Facial recognition is a technology ... system measures the overall facial feature of a ... the distance between eyes. Facial recognition is used ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3
... won the "Most Creative Visualization" award in the "commercial" ... achievement we are extremely proud of. iDEA is Illumina,s ... new and creative visualization and data analysis techniques. ... very first moment we heard about it. Genomatix is ...
... Kan. -- Preliminary research at Kansas State University may make ... scientists believe that cellulose, the most common organic compound on ... biofuels -- if a procedure to effectively break it down ... gives plants their rigidity. Kathrin Schrick, assistant professor in ...
... This press release is available in Spanish ... waters of the European Atlantic Ocean and, nevertheless, resists stock ... the mistakes made by humans. Ms Nerea Goikoetxea, a researcher ... the population dynamics of the northern European hake population, observing ...
Cached Biology News:Learning secrets of world's most common organic compound driving research for biofuels 2Hake population has withstood overfishing, thanks to the warming of the sea 2Hake population has withstood overfishing, thanks to the warming of the sea 3