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WHOLE GENOME SEQUENCING

Agencourt offers whole genome services that can encompass all aspects of genome projects from shotgun library construction through genome annotation and pathway mapping. Our personal project managers consult with clients to design a sequencing strategy to meet their specific project goals and are available to manage the project from start to finish. Our efforts to increase quality while reducing costs allow even the smallest of laboratories to undertake whole genome sequencing endeavors. Agencourt has several features which provide high quality, cost effective genome sequencing including:

  • High performance, fully-automated genomic pipeline
  • Patented SPRI technology
  • Oracle-based Galaxy LIMS
  • Long Phred20 read lengths and high sequencing pass rates
  • Proven experience and provider of sequencing for NHGRI funded whole genome projects

    HIGHLY EXPERIENCED STAFF
    Agencourt's sequencing team has over 75 years of genomics experience from organizations such as The Whitehead Institute, TIGR, Millennium Pharmaceuticals and Cereon Genomics. This staff was instrumental in creating our genomic pipeline and continues to oversee its operation. The combination of our highly experienced staff, fully-automated pipeline, and our patented SPRI technology allows us to process over 20 million Phred20 bases per day with average pass rates in excess of 85%.



    RECOGNIZED HIGH PERFORMANCE FACILITY
    Agencourt is the largest commercial provider of sequencing services performing over 65,000 lanes per day. Our success in marrying the chemistries and instrum entation of high throughput genomics, our background in building a highly scalable sequencing pipeline and our experience managing data from largescale sequencing projects enables us to offer complete genome sequencing with industry-leading quality and the highest level of sample and data integrity.

    LIBRARY CONSTRUCTION STRATEGIES
    Agencourt has experienced personnel dedicated to shotgun library construction and verification. We use unique library construction strategies to provide more random libraries with increased genome coverage which are customized for the specific needs of each project.

    FINISHING AND ASSEMBLY
    Agencourt offers high quality finishing. Our quality standards are among the highest in the industry, with error rates well below 1 in 10,000 nucleotides in the assembled contigs. Our finishing strategies can include custom primer walking or transposon insertion methodologies. We use both commercial and proprietary bioinformatics software packages for finishing and assembly including Phred, PHRAP and GAP 4. Paired end sequencing data allows us to resolve repeat sequences and to maximize the level of contig ordering.

    ANNOTATION
    Highly experienced annotation personnel have the ability to bring the project to full completion with in-depth sequence annotation including nucleotide and amino acid-based homology searches. Several automated annotation options are available including Ab initio gene prediction of genomic sequences, homology searches using BLAST algorithms, and protein domain searches using hidden Markov models.

    PROTEIN-PROTEIN INTERACTION MAPPING
    Age ncourt provides protein-protein interaction mapping services based on a unique bacterial two-hybrid technology. The cost and turnaround time of sequencing and protein mapping projects are greatly reduced by creating genomic sequencing libraries in bacterial two-hybrid vectors, which are fully compatible with high throughput sequencing.

    PROVEN WHOLE GENOME SEQUENCING EXPERIENCE
    Agencourt has taken part in numerous whole genome sequencing efforts including the Human, Mouse, Rat and Tetraodon genome projects along with a host of microbial genome projects. The NHGRI acknowledged our quality and past contributions to the genomics community by designating Agencourt as one of the Large Sequencing Centers to conduct genome sequencing of organisms identified as critical to the advancement of life science research.




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