Navigation Links
Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,,,Mammalian Cells

.3

To demonstrate the quality of both the receptor expression and ecdysone-inducible vectors, we performed transient expression assays using the reporter pEGSH-luc in which the coding sequence for the firefly luciferase gene is inserted into the MCS of pEGSH. In these experiments, reporter was cotransfected with various amounts of the pERV3 receptor vector, induced with murA, and assayed for luciferase activity. Figure 3 shows that, in CHO cells, optimal expression is achieved with a relatively small amount of receptor vector (8 ng) to give an induction ratio of over three orders of magnitude. We achieved comparable results for similar experiments carried out in NIH3T3, 293, and CV-1 cells (data not shown).

Production of Stable Cell Lines

The best method to engineer double-stable cell lines is to first produce stable receptor-expressing cell lines using the plasmid pERV3 and then screen stable receptor-expressing lines by transient transfection of the inducible reporter pEGSH-luc to find one that mediates the highest level of ponA-dependent trans-activation. Once produced, this line can be used for constructing pEGSH-derived lines. To expedite this process, we have produced three engineered cell lines derived from CHO, NIH3T3, and 293 cells, respectively, in which optimal levels of the receptors are stably expressed. These three lines show high-level inducer-dependent activation in transient expression assays using the pEGSH-luc reporter (Figure 4).

Fig.4

Production of Double-Stable Cell Lines

The stable receptor line ER-CHO (Figure 4A ) was stably transfected with the plasmid pEGSH-luc, and hygromycin-
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
4. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. Epitope-Tagging Vectors for Functional Analysis in Yeast
7. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
8. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
9. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
10. High-Titer Retroviral Vectors for Gene Delivery
11. Improved Vectors for PathDetect Trans-Reporting Systems
Post Your Comments:
(Date:4/24/2015)... Telbec/ - Mirego, leader in mobile strategies and solutions, is ... app extensions for the all new Apple Watch. Made in ... and MediaMiser, these extensions will reinvent the way users receive ... wrist use.  Available today in ... tactile smartwatch that makes for an excellent buddy for business, ...
(Date:4/23/2015)... 24, 2015 Ralco announced today that ... Nutrition® (ABN) for the exclusive worldwide rights to sell ... technology is a new in-line milk system that increases ... them to move supplementation milk cups within a farrowing ... “This technology lets producers get Birthright™ milk to the ...
(Date:4/23/2015)... Fla. , April 23, 2015 BioTE ... releases his book "Age Healthier Live Happier - Avoiding ... off the Age Management Medicine Group Conference at JW ... 26, 2015. ",Age Healthier Live Happier, is ... to avoid over-medication and searching for a higher quality ...
(Date:4/23/2015)... 2015 Follow us on ... series of genetic tests that determine individual susceptibility ... disease, Huntington's disease, and Alzheimer's disease. Predictive diagnostics ... and interaction in diseased tissue/cells to understand and ... the response to treatment, such as in the ...
Breaking Biology Technology:Familiprix, OMSignal and MediaMiser apps available today for Apple Watch 2Ralco Acquires License to Birthright™ Moveable Milk Cup 2Release and Book Signing of BioTE Medical President Gary Donovitz M.D. 'Age Healthier Live Happier' at Age Management Medicine Group (AMMG) Conference 2Release and Book Signing of BioTE Medical President Gary Donovitz M.D. 'Age Healthier Live Happier' at Age Management Medicine Group (AMMG) Conference 3Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 2Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 3
... Scientists engaged in biological research and drug discovery are ... BioInformatics, LLC ( www.gene2drug.com ), the premier research ... released preliminary data from the company,s newest report ... Science Suppliers. In this report, we set out ...
... LOUIS, Feb. 11 Sigma-Aldrich, a leading Life,Science and High ... by 6% to $0.68 from $0.64 in the same quarter ... $510 million in the fourth quarter 2008 declined by 4%,compared ... fourth,quarter grew 3% from a year ago. Fourth quarter organic ...
... Development for Chronic Pain IndicationsLA JOLLA, Calif., Feb. ... TPTX ) today announced that oral administration of ... and well-tolerated in healthy male and female subjects ... successful completion of this Phase I trial demonstrating ...
Cached Biology Technology:Life Science Labs Reacting to the Economic Crisis 2Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 2Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 3Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 4Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 5Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 6Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 7Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 8Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 9Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 10Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 11Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 12Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009. 13First Multiple Dose Clinical Trial of TorreyPines Therapeutics' Oral Compound NGX426 Demonstrates Compound Is Safe and Well-Tolerated 2First Multiple Dose Clinical Trial of TorreyPines Therapeutics' Oral Compound NGX426 Demonstrates Compound Is Safe and Well-Tolerated 3First Multiple Dose Clinical Trial of TorreyPines Therapeutics' Oral Compound NGX426 Demonstrates Compound Is Safe and Well-Tolerated 4
(Date:4/20/2015)... 20, 2015 The announcement comes ... Glenbeigh Records Management (GRM), Ireland,s foremost ... Bank. Having built up an impressive track record of clients ... player within the records management sector in Dubai ... is GCC staffbase and employ a further eight staff members ...
(Date:4/13/2015)... 2015 According to a ... Market Forecast & Opportunities, 2020", the global biometrics market ... 14% till 2020. The biometrics market is garnering ... and review of biometric management systems. The constant ... with greater efficiency, are resulting in increasing installation ...
(Date:4/8/2015)... , April 8, 2015  Infinisource, a ... and services, and Morpho, a leading supplier of ... to produce the market,s most advanced biometrically-enabled time ... iSolved NXG time clock is setting a bold, ... labor data for the small and mid-size employer. ...
Breaking Biology News(10 mins):Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3
... by the Duke-NUS Graduate Medical School (Duke-NUS) in Singapore ... key protein linked to stem cell-like behavior in terminal-stage ... drugs that may extend the survival of these patients. ... Proceedings of the National Academy of Sciences , ...
... to reduce by more than half the time it takes ... researchers in Penn State,s College of Agricultural Sciences. The finding ... foodborne illness, allowing epidemiological investigators to identify the exact strains ... more quickly find -- and eliminate -- the source of ...
... long been used for various purposes, like cleaning wastewater at ... handcrafted wickerwork, as means of nutrition and, in traditional medicine, ... Fraunhofer Institute for Building Physics IBP in Valley now want ... to wit, for the insulation of outer walls or ...
Cached Biology News:Singapore research team identifies new drug target in deadly form of leukemia 2Researchers develop a faster method to identify Salmonella strains 2Researchers develop a faster method to identify Salmonella strains 3Using cattails for insulation 2Using cattails for insulation 3
RABBIT ANTI RHEB (IN) Immunogen: A 15 amino acid peptide located in the central region of human Rheb....
Rabbit polyclonal antibody to Neuromedin U Receptor 1 (GPR66)...
... is causing shortages in your laboratory or fellow ... unwanted distractions, problems and delays. • ... and endeavour! Bring us your idea and let ... We work under ISO 9001 quality assurance in ...
... a recombinant preparation of the catalytic subunit ... expressed and isolated from the yeast Pichia ... preparation (2). It recognizes the sequence Asp-Asp-Asp-Asp-Lys ... lysine residue. The enzyme can be used ...
Biology Products: