Navigation Links
Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,,,Mammalian Cells

of the CMV promoter; and the CMV promoter in this construct can be readily replaced with other promoters to confer cell-type specificity to receptor expressionan advantage that is particularly attractive for the construction of transgenic animals. The plasmid also contains a neomycin-resistance gene, which is expressed in both E. coli (kanamycin-resistance) and mammalian cells (G418-resistance).

Fig.2

The ecdysone-inducible expression vector pEGSH contains the ponA-inducible expression cassette comprised of 5 E/GRE binding sites upstream of a minimal promoter consisting of three SP1 sites, followed by the D. melanogaster hsp27 minimal promoter (Figure 2B ). The vector contains the hygromycin-resistance gene to allow stable selection in cells transformed with the pERV3 plasmid. The MCS of pEGSH was engineered to contain an array of restriction sites positioned for directional cloning of inserts derived from Lambda ZAP-derived cDNA vectors*, lgt10, lgt11, HybriZAP vectors, and other two-hybrid libraries, in addition to most other popular cDNA cloning and expression vectors.

In addition to this versatile array of restriction sites, the pEGSH MCS contains the following features: the ability to monitor expression of the gene of interest, by either a-FLAG immunodetectionll ll or by RNA detection using T3 antisense RNA probes; and the ability to seamlessly fuse the insert to the FLAG epitope and/or the HSP leader by using the seamless cloning kit** for which 80% cloning efficiencies are routinely achieved.

Transient Expression Assays

Fig
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
4. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. Epitope-Tagging Vectors for Functional Analysis in Yeast
7. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
8. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
9. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
10. High-Titer Retroviral Vectors for Gene Delivery
11. Improved Vectors for PathDetect Trans-Reporting Systems
Post Your Comments:
(Date:1/15/2014)... RedBrick Health , a fast-growing leader in consumer ... EmblemHealth , the New York ... the RedBrick Compass health assessment, combined with the RedBrick ... members. EmblemHealth is among the first health plans in ...
(Date:1/15/2014)... January 15, 2014 The Microcompetition with Foreign ... disease. One of these latent viruses is the Epstein Barr ... (RA). Rheumatoid arthritis (RA) is a chronic inflammatory disease that ... study found that RA patients have high concentrations of EBV ...
(Date:1/15/2014)... TaiGen Biotechnology Company, Limited ("TaiGen") today announced that they ... Russian pharmaceutical company, to develop and commercialize nemonoxacin (Taigexyn ... , Turkey and other members ... antibiotic for the treatment of bacterial infections including those ...
(Date:1/14/2014)... As pet owners drew up their lists of New Year,s ... better care of my furry companion." Nowadays there are pet salons, ... cases to take the little canine or feline darlings along wherever ... buy some pricey toys at the pet store. But anyone who ...
Breaking Biology Technology:EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... SEATTLE, April 22 Trubion Pharmaceuticals, Inc. (Nasdaq: ... preclinical data regarding its SCORPION(TM) multispecific therapeutic technology ... Association of Cancer Research (AACR) in Denver. The ... of the bispecific CD79BxDR SCORPION molecule, its differentiation ...
... tasting rooms really help generate sales for wineries? According ... almost half of winery tasting room visitors purchase the ... "This research proves that the tasting room experience does ... sales," said Dr. Isabelle Lesschaeve, research director, consumer insights ...
... Microtest Laboratories ( www.microtestlabs.com ... to expand its position as a proven, one-stop provider ... services for manufacturers of medical devices or pharmaceuticals.Microtest ... ensure regulatory compliance as well as to achieve greater ...
Cached Biology Technology:Trubion Presents Preclinical Data on Its SCORPION(TM) Multispecific Therapeutics at the 2009 American Association of Cancer Research Annual Meeting 2Trubion Presents Preclinical Data on Its SCORPION(TM) Multispecific Therapeutics at the 2009 American Association of Cancer Research Annual Meeting 3Trubion Presents Preclinical Data on Its SCORPION(TM) Multispecific Therapeutics at the 2009 American Association of Cancer Research Annual Meeting 4Setting the Benchmark for Winery Tasting Room Research: An Emerging Region Gets a Global Perspective 2Microtest Labs Expands Clean Room, Contamination Testing Services 2Microtest Labs Expands Clean Room, Contamination Testing Services 3Microtest Labs Expands Clean Room, Contamination Testing Services 4
(Date:4/17/2014)... bone marrow need to produce hydrogen sulfide in order ... a new study from the Center for Craniofacial Molecular ... USC. , Professor Songtao Shi, principal investigator on the ... the cells governs the flow of calcium ions. The ... results in osteogenesis, or the creation of new bone ...
(Date:4/17/2014)... in Europe, Asia and the United States are spreading ... impacting native honeybee populations at this time, according to ... including Nosema microsporidia and Varroa ... to these invasive pests, which suggests to us that ... and the United States currently are not necessary in ...
(Date:4/17/2014)... IPCC meeting painted a stark warning on the possible ... a greenhouse effect 32 times that of carbon dioxide. ... humic substances act as fully regenerable electron acceptors which ... in wetlands instead of being released to the atmosphere. ... disrupted it may enter into a vicious cycle to ...
Breaking Biology News(10 mins):East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3Methane climate change risk suggested by proof of redox cycling of humic substances 2
... discovered patterns of brain activity that may underlie our remarkable ... Computers can beat us at math and chess, but ... Web sites use object recognition tasks as part of their ... describe a teapot as having a C-shaped handle on one ...
... October 23, 2008 The Soil Science Society of America ... Meetings on Oct. 5-9 in Houston, TX. ... Frederick Soil Teaching Travel Study Award. Bruno Basso is ... Forestry and Environmental Sciences at the University of Basilicata ...
... John Innes Centre in Norwich, Great Britain, in collaboration ... have obtained genetically modified tomatoes rich in anthocyanins, a ... These tomatoes, added to the diet of cancer-prone mice, ... lifespan. The research has been published in the 26 ...
Cached Biology News:How we see objects in depth: The brain's code for 3-D structure 2How we see objects in depth: The brain's code for 3-D structure 3Soil Science Society of America presents awards in Houston 2Soil Science Society of America presents awards in Houston 3Soil Science Society of America presents awards in Houston 4Soil Science Society of America presents awards in Houston 5Soil Science Society of America presents awards in Houston 6Purple tomatoes: The richness of antioxidants against tumors 2Purple tomatoes: The richness of antioxidants against tumors 3
... Immunogen KLH-conjugated, synthetic ... 47-66 (PAEV-GPGAVGERTPRKKEPP) of human BRAF-HDAC ... (BHC110/LSD1). Quality Assurance ... Stability 2 years ...
...
Rabbit anti-CUGBP2...
Request Info...
Biology Products: