Navigation Links
Versatile Transfection Reagent Offers Low Toxicity and Consistent,,,Performance

4

A quantitative analysis of transfection efficiencies is shown for LipofectAMINE reagent (Figure 4A) and LipoTAXI reagent (Figure 4B). For all tested conditions, LipoTAXI transfection reagent yielded higher transfection efficiencies of SW13 cells with the pEGFP-C3 plasmid. In addition, cell death, indicated by floating cells, occurred with greater frequency with increasing concentrations of LipofectAMINE reagent. Increased cell death was not seen with increasing concentrations of LipoTAXI reagent, again demonstrating LipoTAXIs low toxicity.

Conclusions

Optimizing transfection conditions is a cell-line dependent process and should be performed for each cell line studied. When comparing efficiencies using four different lipid reagents for transfections in A549, COS-7, and NIH 3T3 cells, Stratagenes LipoTAXI transfection reagent consistently gave the highest transfection efficiencies and the lowest cellular toxicity. Atypical optimization profiles and low transfection efficiencies can be caused by mycoplasma contamination of cell lines, which is easily detected with Stratagenes Mycoplasma PCR Primer Set. LipoTAXI transfection reagent is significantly more effective than LipofectAMINE reagent for transfecting enhanced green fluorescent protein into SW13 cells, as measured by transfection efficiency and cell death. Furthermore, LipoTAXI transfection reagent is effective over a broader range of conditions than LipofectAMINE reagent leading to less cell death. Therefore, LipoTAXI transfection reagent is superior for consistent, low-toxicity, high-efficiency transfections.

REFERENCES
  1. Ausubel, F.M., et al. (1994) In Current Protocols in Molecular Biology. Wiley & Sons, New York.

  2. Jordan, M., Schallhorn, A., and
    '"/>



Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Versatile Reporter Vectors for Monitoring Viral Transduction
4. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
10. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
11. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
Post Your Comments:
(Date:10/1/2014)... 30, 2014 In response to ... to address sales, training and consumer engagement challenges, ... innovative mobile applications to turn their clients’ visions ... latest customized mobile app creations, are now featured ... In partnership with Toyota, StudioPMG created a robust ...
(Date:10/1/2014)... (PRWEB) October 01, 2014 The U.S. ... to Southern Illinois University Edwardsville as part of the ... initiative. The funding is co-administered by the Department of ... Biden, Secretary of Labor Thomas E. Perez and Secretary ... million in job-driven training grants to nearly 270 colleges ...
(Date:9/30/2014)... German . ... have demonstrated a new kind of building block for ... chips could be based on three-dimensional arrangements of nanometer-scale ... of the semiconductor industry CMOS fabrication of silicon ... collaborators at the University of Notre Dame are exploring ...
(Date:9/30/2014)... Sept. 30, 2014 Nematicide is a ... Nematodes are microscopic parasitic roundworms, found in massive ... other plants and animals. Nematicides have tended to ... properties promoting migration through the soil. A single ... plant-parasitic nematodes. With more than 10,000 species classified, ...
Breaking Biology Technology:Digital Agency Studio PMG Launches New Case Studies on Website 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 3U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 4A new dimension for integrated circuits: 3-D nanomagnetic logic 2A new dimension for integrated circuits: 3-D nanomagnetic logic 3Nematicides Market by Types (Fumigants, Organophosphates, Carbamate and Bio-Nematicides), Applications (Agrochemicals, Industrial, and Others), and Geography (North America, Europe, Asia-Pacific & ROW) - Global Trends & Forecasts to 2019 2
... conference at Grainger Hall. Madison, Wis. ... Wisconsin, according to the Coalition for Capital Growth and Research, ... Hall. , ,We believe [venture capital] is a worthwhile agenda ... partner at Venture Investors and the conferences founder. ...
... a look at how the large cap Midwest life science companies ... stock market. The results looked very positive for at least 75 ... market growth. , ,On the negative side, some of the poor ... our largest Big Pharma companies (namely Abbott Labs and Eli Lilly). ...
... I never cease to be amazed by the games that ... to goose up revenues and shore up profits. Most of ... Lately, things have gotten ridiculous enough to make one yearn ... calls. , ,And Im not talking about the nuisance of ...
Cached Biology Technology:Wisconsin investors seek regional partners and informed legislature 2Wisconsin investors seek regional partners and informed legislature 3Midwest small-cap life sciences turn in mixed third-quarter results 2Show customers leadership, not gamesmanship 2Show customers leadership, not gamesmanship 3
(Date:10/1/2014)... Finland , October 1, 2014 ... through in the life science field  ... technologies come and go over the years. Some ... standard. Photon upconversion is a novel detection technology ... recent years. Now, finally, this technology is becoming ...
(Date:9/30/2014)... Millions of tourists visit the Mediterranean each year, ... currently underway on Earth. Almost 1,000 alien species, ... from other seas through human activities. In the ... a multinational team of researchers analyzed data from ... Commission to show how the introduction of alien ...
(Date:9/30/2014)... California, San Diego, have completed the first comprehensive numerical ... uses the pixels in an image as data points ... , The researchers, led by J.S. Chen, the William ... of Engineering at UC San Diego, presented their findings ... conference in Pittsburgh this month. Chen also gave a ...
Breaking Biology News(10 mins):First-of-a-kind Solution for Utilizing Photon Upconversion 2Biodiversity in the Mediterranean is threatened by alien species 2First comprehensive meshfree numerical simulation of skeletal muscle tissue achieved 2
... study shows that human milk oligosaccharides, or HMO, produce short-chain ... infant gut. Not only that, the bacterial composition adjusts as ... though HMO are a major component of human milk, present ... the infant are not well understood. Furthermore, they,re virtually absent ...
... JOLLA, CA ---- Scientists at the Salk Institute for ... of plant proteins that play a role in the ... human nutrition, biorenewable chemicals and biofuels. Scoring a ... related proteins in thale cress plants ( Arabidopsis thaliana ...
... thanks to a paper-thin generator embedded in the sole ... little closer to reality. Scientists from the U.S. Department ... developed a way to generate power using harmless viruses ... tested their approach by creating a generator that produces ...
Cached Biology News:Study sheds new light on importance of human breast milk ingredient 2Discovery of plant proteins may boost agricultural yields and biofuel production 2Discovery of plant proteins may boost agricultural yields and biofuel production 3Berkeley Lab scientists generate electricity from viruses 2Berkeley Lab scientists generate electricity from viruses 3
... The FastPlax Antibody is used to ... on the surface of infected cells as ... the basis of the FastPlax Titer Kit, ... just 25-48 hours. The FastPlax ...
Anti-Mouse Ly 6C.2 , Ascites (Clone 5075-3.6) (mouse IgG2b)...
b-Catenin (pSer675), phospho-specific...
Goat polyclonal to SIAH Interacting Protein ( Abpromise for all tested applications)....
Biology Products: